In vitro effects of thyroxine on the mechanical properties of erythrocytes.

In vitro effects of thyroxine on erythrocyte deformability and mechanical fragility were observed. Deformability of erythrocytes was improved in a dose dependent manner by thyroxine. Mechanical hemolysis was found to be lower if thyroxine was included in erythrocyte suspensions at concentrations close to the physiological levels (10(-9)M). These changes might be related to the alterations of intracellular calcium concentration, as in the erythrocyte suspensions containing 10(-9)M thyroxine, intracellular calcium concentration was found to be 30 times lower than the control suspensions which did not contain thyroxine. Thyroxine also reduced the mechanical hemolysis ratio in calcium loaded cells. These observations suggest that thyroxine might play some role in the regulation of the mechanical properties of erythrocytes which might be mediated via the effects on calcium metabolism.

[1]  T. Kato,et al.  Na-K-dependent ATPase in red cells and thyroid status. , 1982, Endocrinologia japonica.

[2]  N. Tietz.  Fundamentals of Clinical Chemistry , 1970 .

[3]  R. Waddell,et al.  Alteration in intracellular sodium concentration and ouabain-sensitive ATPase in erythrocytes from hyperthyroid patients. , 1976, The Journal of clinical endocrinology and metabolism.

[4]  L. McIntire,et al.  Reduced erythrocyte deformability associated with calcium accumulation. , 1982, Biochimica et biophysica acta.

[5]  F. B. Davis,et al.  Thyroid hormone stimulation in vitro of red blood cell Ca2+-ATPase activity: interspecies variation. , 1982, Endocrinology.

[6]  P. Davis,et al.  In vitro stimulation of human red blood cell Ca2+-ATPase by thyroid hormone. , 1981, Biochemical and biophysical research communications.

[7]  J. Stuart.  The Function of Red Blood Cells: Erythrocyte Pathobiology , 1982, Progress in clinical and biological research.

[8]  P. Vítek,et al.  The haematology of hyperthyroidism. , 1978, The Quarterly journal of medicine.

[9]  J. England,et al.  The haematology of hypothyroidism. , 1976, The Quarterly journal of medicine.

[10]  N. Mohandas,et al.  Erythrocyte membrane deformability and stability: two distinct membrane properties that are independently regulated by skeletal protein associations , 1986, The Journal of cell biology.

[11]  P. D. Bewsher,et al.  A search for the mechanism underlying the altered MCV in thyroid dysfunction: a study of serum and red cell membrane lipids. , 2009, Scandinavian journal of haematology.

[12]  S. Shohet,et al.  Red cell biochemical anatomy and membrane properties. , 1987, Annual review of physiology.