A novel dodecadepsipeptide, cereulide, is an emetic toxin of Bacillus cereus.

A vacuole-formation substance, cereulide of Bacillus cereus, is an emetic toxin in animals. Both oral administration and intraperitoneal injection of cereulide caused dose-dependent emesis in Suncus murinus, a new animal model of emesis. Vagotomy or a 5-HT3 receptor antagonist completely abolished this emetic effect. Therefore, cereulide causes emesis through the 5-HT3 receptor and stimulation of the vagus afferent. We also found that our purified cereulide caused swelling of mitochondria of HEp-2 cells.

[1]  M. Mori,et al.  Structure of cereulide, a cyclic dodecadepsipeptide toxin from Bacillus cereus and studies on NMR characteristics of its alkali metal complexes including a conformational structure of the K+ complex , 1995 .

[2]  M. Mori,et al.  A novel dodecadepsipeptide, cereulide, isolated from Bacillus cereus causes vacuole formation in HEp-2 cells. , 1994, FEMS microbiology letters.

[3]  H. Hayashi,et al.  The Rice Culture Filtrate of Bacillus cereus Isolated from Emetic‐Type Food Poisoning Causes Mitochondrial Swelling in a HEp‐2 Cell , 1994, Microbiology and immunology.

[4]  R. Szabo,et al.  Cell Culture Detection and Conditions for Production of a Bacillus cereus Heat-Stable Toxin. , 1991, Journal of food protection.

[5]  N. Matsuki,et al.  Selective blockade of cytotoxic drug-induced emesis by 5-HT3 receptor antagonists in Suncus murinus. , 1991, Japanese journal of pharmacology.

[6]  J. Kramer,et al.  Potential application of a HEp‐2 cell assay in the investigation of Bacillus cereus emetic‐syndrome food poisoning , 1988 .

[7]  N. Matsuki,et al.  Suncus murinus: a new experimental model in emesis research. , 1987, Life sciences.

[8]  B. Costall,et al.  5-hydroxytryptamine m-receptor antagonism to prevent cisplatin-induced emesis , 1986, Neuropharmacology.

[9]  G. Sanger,et al.  Inhibition of cisplatin‐induced vomiting by selective 5‐hydroxytryptamine M‐receptor antagonism , 1986, British journal of pharmacology.

[10]  R. J. Gilbert,et al.  Bacillus cereus enterotoxins: present status. , 1984, Biochemical Society transactions.

[11]  J. Melling,et al.  Characteristics of Bacillus cereus emetic toxin , 1978 .

[12]  R. J. Gilbert,et al.  Identification of a novel enterotoxigenic activity associated with Bacillus cereus. , 1976, Journal of clinical pathology.