Optical bio-sniffer for ethanol vapor using an oxygen-sensitive optical fiber.

An optical bio-sniffer for ethanol was constructed by immobilizing alcohol oxidase (AOD) onto a tip of a fiber optic oxygen sensor with a tube-ring, using an oxygen sensitive ruthenium organic complex (excitation, 470 nm; fluorescent, 600 nm). A reaction unit for circulating buffer solution was applied to the tip of the device. After the experiment in the liquid phase, the sniffer-device was applied for gas analysis using a gas flow measurement system with a gas generator. The optical device was applied to detect the oxygen consumption induced by AOD enzymatic reaction with alcohol application. The sensor in the liquid phase was used to measure ethanol solution from 0.50 to 9.09 mmol/l. Then, the bio-sniffer was calibrated against ethanol vapor from 0.71 to 51.49 ppm with good gas-selectivity based on the AOD substrate specificity. The bio-sniffer with the reaction unit was also used to monitor the concentration change of gaseous ethanol by rinsing and cleaning the fiber tip and the enzyme membrane with buffer solution.

[1]  Isao Karube,et al.  Analysis of metabolites in sweat as a measure of physical condition , 1994 .

[2]  I. Karube,et al.  Gas-Phase Biosensor for Ethanol , 1994 .

[3]  Norio Miura,et al.  Development of SnO2-based ethanol gas sensor , 1992 .

[4]  K. Ichimura.  A convenient photochemical method to immobilize enzymes , 1984 .

[5]  Mahmoud R. Shahriari,et al.  Highly sensitive, all solid state fibre optic oxygen sensor based on the sol-gel coating technique , 1996 .

[6]  J. Olmsted,et al.  Mechanism of quenching of electronically excited ruthenium complexes by oxygen , 1989 .

[7]  K. Mitsubayashi,et al.  Development of a Gas-Phase Biosensor for Trimethylamine Using a Flavin-Containing Monooxygenase 3 , 2000 .

[8]  S. Terry,et al.  A gas chromatographic air analyzer fabricated on a silicon wafer , 1979, IEEE Transactions on Electron Devices.

[9]  Sinclair S. Yee,et al.  An integrated array of multiple thin-film metal oxide sensors for quantification of individual components in organic vapor mixtures , 1993 .

[10]  C. McDonagh,et al.  LED-based fibre optic oxygen sensor using sol-gel coating , 1994 .

[11]  K. Mitsubayashi,et al.  Bioelectronic Nose for Methyl Mercaptan Vapor using a Xenobiotic Metabolizing System , 2001 .

[12]  K. Colbow,et al.  MgO-doped Cr2O3: solubility limit and the effect of doping on the resistivity and ethanol sensitivity , 1992 .

[13]  Y. Hiranaka,et al.  Gas-dependent response in the temperature transient of SnO2 gas sensors☆ , 1992 .

[14]  James N. Demas,et al.  Determination of oxygen concentrations by luminescence quenching of a polymer-immobilized transition-metal complex , 1987 .

[15]  G. Preti,et al.  Oral odors in early experimental gingivitis. , 1984, Journal of periodontal research.