Acute erythroleukaemia with L3 morphology and the 14q+ chromosome.

L3 morphology according to the FAB classification and the 14q+ chromosome are usually ascribed to the Burkitt type of leukaemia or lymphoma with a B or pre-B cell phenotype. We report here a case of adult acute leukaemia with Burkitt morphology and the 14q+, which did not express lymphoid markers. Instead, the leukaemia was shown to be an acute erythroleukaemia. The erythrocyte marker glycophorin A was present on the surface of the leukemic blasts as shown by immunofluorescence and immunoprecipitation from membrane lysates of surface labeled cells with antiglycophorin A antiserum. Spectrin and fetal hemoglobin appeared after cultivation of the blasts in the presence of sodium butyrate. The present case shows that a short term cultivation is sometimes useful for further characterization of the commitment of acute leukaemias.

[1]  C. Gahmberg,et al.  Surface glycoproteins of malignant human leukocytes. , 1982, Biochimica et biophysica acta.

[2]  J. Rowley.  Association of specific chromosome abnormalities with type of acute leukemia and with patient age. , 1981, Cancer research.

[3]  A. de la Chapelle,et al.  Culture of bone marrow reveals more cells with chromosomal abnormalities than the direct method in patients with hematologic disorders. , 1981, Blood.

[4]  R. Berger,et al.  Cytogenetic studies on African Burkitt's lymphoma cell lines: t(8;14), t(2;8) and t(8;22) translocations. , 1981, Cancer genetics and cytogenetics.

[5]  P. Edwards,et al.  Expression of cell-surface HLA-DR, HLA-ABC and glycophorin during erythroid differentiation , 1981, Nature.

[6]  J. Rowley.  CHROMOSOME CHANGES IN ACUTE LEUKAEMIA , 1980, British journal of haematology.

[7]  C. Gahmberg,et al.  Glycophorin a as a cell surface marker of early erythroid differentiation in acute leukemia , 1979, International journal of cancer.

[8]  H. Berghe,et al.  Variant translocation in Burkitt lymphoma , 1979 .

[9]  C. Gahmberg,et al.  Induction of erythroid differentiation in the human leukaemia cell line K562 , 1979, Nature.

[10]  C. Gahmberg,et al.  K562—A human erythroleukemic cell line , 1979, International journal of cancer.

[11]  C. Gahmberg,et al.  Expression of the major sialoglycoprotein (glycophorin) on erythroid cells in human bone marrow , 1978 .

[12]  L. Andersson,et al.  Surface glycoproteins of human white blood cells. Analysis by surface labeling. , 1978, Blood.

[13]  C. Gahmberg,et al.  Selective radioactive labeling of cell surface sialoglycoproteins by periodate-tritiated borohydride. , 1977, The Journal of biological chemistry.

[14]  H. Gralnick,et al.  Proposals for the Classification of the Acute Leukaemias French‐American‐British (FAB) Co‐operative Group , 1976, British journal of haematology.

[15]  G. Klein,et al.  Characteristic chromosomal abnormalities in biopsies and lymphoid‐cell lines from patients with burkitt and non‐burkitt lymphomas , 1976, International journal of cancer.

[16]  W. Bonner,et al.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. , 1974, European journal of biochemistry.

[17]  C. Gahmberg,et al.  External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes. , 1973, The Journal of biological chemistry.

[18]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[19]  P. Owren,et al.  A controlled trial of the effect of linolenic acid on incidence of coronary heart disease. The Norwegian vegetable oil experiment of 1965-66. , 1968, Scandinavian journal of clinical and laboratory investigation. Supplementum.

[20]  F. Greenwood,et al.  THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. , 1963, The Biochemical journal.