Migration and internalization of cells and polystyrene microsphere in tumor cell spheroids.

Abstract The movement and internalization of 3H-labelled cells and of inert polystyrene microspheres within multicellular spheroids has been examined through histological sectioning and autoradiography. EMT6 and RIF-1 spheroids were cultured in spinner flasks for approx. 2.5 weeks. At this time, 3H-labelled cells and/or microspheres were allowed to adhere to the spheroid surface. Microspheres, 3H-labelled RIF-1 monolayer cells and 3H-labelled EMT6 monolayer cells were observed to move centripetally as a wave into EMT6 spheroids. In contrast, 3H-labelled trypsinized RIF-1 and EMT6 spheroid cells became mixed with the other non-labelled spheroid cells in homotypic RIF-1 and EMT6 spheroids, respectively. Reduction of spheroid growth by maintaining the spheroids at room temperature and by treatment with 2500 rads irradiation did not prohibit the internalization of 3H-labelled EMT6 cells and microspheres in EMT6 spheroids.

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