论文信息 - Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells

Parylene membrane slot filter for the capture, analysis and culture of viable circulating tumor cells

This paper presents a method of capturing viable circulating tumor cells (CTC) from human whole blood using constant-pressure-driven filtration through a specially designed parylene-C membrane “slot” filter. More than 90% viable cancer cells could be recovered from whole blood using the slot filter, with minimal non-cancer blood cells left on the filter. The feasibility of the telomerase activity measurement of a single cancer cell taken from the filter after capture was proven. The on-filter and off-filter cultures of the captured cancer cells were also demonstrated.

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