STED imaging of cytoplasmic RNA in live cells

RNAs play pivotal roles in many biological processes, such as translation, gene editing etc. Since RNAs are usually smaller in size than optical diffraction limit, it is impossible for optical microscopes to get deep insight of them. With the recent emergence of super-resolution optical imaging microscopies, it becomes possible to study the ultra-fine structure of such RNA in side cells. Different kind of fluorescent probes are used for RNA labeling and imaging inside cells. However, most of them cannot be used for live-cell super-resolution imaging, due to typical laser toxicity to live cells. Herein, we developed a new type of RNA-labeled probe, which can be used for STED imaging of RNA in living cells. We believe this probe is of great significance for studying biological behaviors of RNA in living cells.

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