Islets of Langerhans: dye coupling among immunocytochemically distinct cell types.

Cells in isolated rat islets of Langerhans were microinjected with Lucifer Yellow CH in a medium containing 16.7 millimolar glucose. Dye was rapidly transferred from the injected cell to neighboring islet cells without specificity with regard to the immunocytochemical identity of either the donor or the recipient cells. The transfer of dye between the islet cells (types A, B, and D) demonstrates homologous and heterologous cell coupling in a system where the normal proportions and relationships of the cell types are maintained.

[1]  E. Yavin,et al.  ATTACHMENT AND CULTURE OF DISSOCIATED CELLS FROM RAT EMBRYO CEREBRAL HEMISPHERES ON POLYLYSINE-COATED SURFACE , 1974, The Journal of cell biology.

[2]  L Orci,et al.  Endocrine pancreas: three-dimensional reconstruction shows two types of islets of langerhans. , 1979, Science.

[3]  L Orci,et al.  Cell contacts in human islets of Langerhans. , 1975, The Journal of clinical endocrinology and metabolism.

[4]  J. N. Hayward,et al.  Intracellular dye-marked enkephalin neurons in the magnocellular preoptic nucleus of the goldfish hypothalamus. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[5]  L Orci,et al.  Gap junction development is correlated with insulin content in the pancreatic B cell. , 1980, Science.

[6]  R. Williams,et al.  Effect of Glucose Concentration on Insulin and Glucagon Release from Isolated Islets of Langerhans of the Rat , 1968, Diabetes.

[7]  L Orci,et al.  Increase of gap junctions between pancreatic B-cells during stimulation of insulin secretion , 1979, The Journal of cell biology.

[8]  W. W. Stewart,et al.  Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer , 1978, Cell.

[9]  B. Lane,et al.  DIFFERENTIAL STAINING OF ULTRATHN SECTIONS OF EPON-EMBEDDED TISSUES FOR LIGHT MICROSCOPY , 1965, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[10]  P. Lacy,et al.  Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas , 1967, Diabetes.

[11]  J. Sheridan,et al.  Junctions between Cancer Cells in Culture: Ultrastructure and Permeability , 1971, Science.

[12]  E Kohen,et al.  Intercellular communication in pancreatic islet monolayer cultures: a microfluorometric study. , 1979, Science.

[13]  F. Clementi,et al.  Intercellular junctions of oral epithelium. II. Ultrastructural changes in rat buccal epithelium induced by trypsin digestion. , 1977, Journal of ultrastructure research.

[14]  H. Meissner.  Electrophysiological evidence for coupling between β cells of pancreatic islets , 1976, Nature.