Bacterial density and survey of cultivable heterotrophs in the surface water of a freshwater marsh habitat of Anopheles quadrimaculatus larvae (Diptera: Culicidae).

We examined surface water samples collected in September and October 1994 from a freshwater marsh habitat containing larval Anopheles quadrimaculatus mosquitoes. Bacterial densities in direct microscopic counts ranged from 9.7 x 10(5) to 1.3 x 10(7) cells/ml. Densities of cultivable bacteria on trypticase soy agar medium ranged from 1.0 to 1.5 x 10(5) cells/ml. The majority of 888 isolates were gram-positive rods (41%) followed by gram-negative rods (28%). Analysis of the cellular fatty acid profiles of 824 isolates using gas chromatography and Microbial Identification Systems TSBA (Rev. 3.60) library software grouped the bacteria into Bacillus spp. (35%), other gram-positive bacteria (16%), pseudomonads (15%), other gram-negative bacteria including mainly Enterobacteriaceae and Vibrionaceae (21%), and profiles not recognized (13%). Among 33 genera within these groups, the most common were Bacillus, Pseudomonas, Aeromonas, and Arthrobacter.

[1]  E. Walker,et al.  Effects of variation in quality of leaf detritus on growth of the eastern tree-hole mosquito, Aedes triseriatus (Diptera: Culicidae) , 1997 .

[2]  R. Wotton,et al.  Growth of Anopheles mosquito larvae on dietary microbiota in aquatic surface microlayers , 1997, Medical and veterinary entomology.

[3]  E. Walker,et al.  Feeding behavior of aquatic insects: Case studies on black fly and mosquito larvae , 1996 .

[4]  J. Pratt,et al.  Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present. , 1994, Microbiological reviews.

[5]  T. Sota,et al.  Bacteria as Diet for the Mosquito Larvae Aedes (Stegomyia) (Diptera: Culicidae) : Preliminary Experiments with Pseudomonas fluorescens , 1994 .

[6]  A. Porter,et al.  Mosquitocidal toxins of bacilli and their genetic manipulation for effective biological control of mosquitoes. , 1993, Microbiological reviews.

[7]  E. Walker,et al.  Bacterial enrichment in the surface microlayer of an Anopheles quadrimaculatus (diptera: culicidae) larval habitat. , 1993, Journal of medical entomology.

[8]  N. T. Marsac,et al.  Occurrence and abundance of cyanobacteria in a brackish marshland and their ingestibility by mosquito larvae , 1993 .

[9]  J. Bausch,et al.  Lipid analysis. , 1993, Current opinion in biotechnology.

[10]  J. Andrews,et al.  Enumeration and Characterization of Bacterial Colonists of a Submersed Aquatic Plant, Eurasian Watermilfoil (Myriophyllum spicatum L.) , 1992, Applied and environmental microbiology.

[11]  D. Stead,et al.  Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria , 1992 .

[12]  S. Brenner,et al.  Expression of the mosquitocidal toxins of Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis by recombinant Caulobacter crescentus, a vehicle for biological control of aquatic insect larvae , 1992, Applied and environmental microbiology.

[13]  E. Walker,et al.  Feeding behavior, natural food, and nutritional relationships of larval mosquitoes. , 1992, Annual review of entomology.

[14]  M. Höfle.  Bacterioplankton community structure and dynamics after large-scale release of nonindigenous bacteria as revealed by low-molecular-weight-RNA analysis. , 1992, Applied and environmental microbiology.

[15]  K. Porter,et al.  Differential survival of bacteria ingested by zooplankton from a stratified eutrophic lake , 1991 .

[16]  E. Walker,et al.  Natural food and feeding behavior of Coquillettidia perturbans larvae. , 1990, Journal of the American Mosquito Control Association.

[17]  D C White,et al.  Lipid analysis in microbial ecology: quantitative approaches to the study of microbial communities. , 1989, Bioscience.

[18]  C. Reynolds.  Physical Determinants of Phytoplankton Succession , 1989 .

[19]  E. Walker,et al.  Analysis of the Distribution and Abundance of Anopheles quadrimaculatus (Diptera: Culicidae) Larvae in a Marsh , 1988 .

[20]  E. Walker,et al.  Gut content analysis of mosquito larvae (Diptera: Culicidae) using DAPI stain and epifluorescence microscopy. , 1988, Journal of medical entomology.

[21]  M. Laird.  The Natural History of Larval Mosquito Habitats , 1988 .

[22]  R. Colwell,et al.  Survival strategies of bacteria in the natural environment. , 1987, Microbiological reviews.

[23]  J. Fry,et al.  Viability of Heterotrophic Bacteria in Freshwater , 1982 .

[24]  D. Kirchman,et al.  Contribution of Particle-Bound Bacteria to Total Microheterotrophic Activity in Five Ponds and Two Marshes , 1982, Applied and environmental microbiology.

[25]  K. Porter,et al.  The use of DAPI for identifying and counting aquatic microflora1 , 1980 .

[26]  A. J. Ramsay.  Aerobic heterotrqphic bacteria isolated from water, mud, and macrophytes of Lake Grasmere, New Zealand , 1977 .

[27]  A. J. Ramsay.  Heterotrophic bacteria and their relationship with plankton in a New Zealand freshwater lake , 1976 .

[28]  R. S. Harvey,et al.  Temperature influence on bacterial populations in three aquatic systems , 1974 .

[29]  A. D. Hess,et al.  The Intersection Line as a Factor in Anopheline Ecology. , 1943 .