Charge, size, and cellular selectivity for multiwall carbon nanotubes by maize and soybean.

Maize (Zea mays) and soybean (Glycine max) were used as model food-chain plants to explore vegetative uptake of differently charged multiwall carbon nanotubes (MWCNTs). Three types of MWCNTs, including neutral pristine MWCNT (p-MWCNT), positively charged MWCNT-NH2, and negatively charged MWCNT-COOH, were directly taken-up and translocated from hydroponic solution to roots, stems, and leaves of maize and soybean plants at the MWCNT concentrations ranging from 10.0 to 50.0 mg/L during 18-day exposures. MWCNTs accumulated in the xylem and phloem cells and within specific intracellular sites like the cytoplasm, cell wall, cell membrane, chloroplast, and mitochondria, which was observed by transmission electron microscopy. MWCNTs stimulated the growth of maize and inhibited the growth of soybean at the exposed doses. The cumulative transpiration of water in maize exposed to 50 mg/L of MWCNT-COOHs was almost twice as much as that in the maize control. Dry biomass of maize exposed to MWCNTs was greater than that of maize control. In addition, the uptake and translocation of these MWCNTs clearly exhibited cellular, charge, and size selectivity in maize and soybean, which could be important properties for nanotransporters. This is the first report of cellular, charge, and size selectivity on the uptake by whole food plants for three differently charged MWCNTs.

[1]  Maged F. Serag,et al.  Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells. , 2011, ACS nano.

[2]  Tonghua Wang,et al.  Translocation and fate of multi-walled carbon nanotubes in vivo , 2007 .

[3]  Alberto Bianco,et al.  Carbon nanotubes for the delivery of therapeutic molecules , 2004, Expert opinion on drug delivery.

[4]  Arturo A. Keller,et al.  Global life cycle releases of engineered nanomaterials , 2013, Journal of Nanoparticle Research.

[5]  J. Schnoor,et al.  Phytotoxicity of chlorinated aliphatics to hybrid poplar (Populus deltoides × nigra DN34) , 2001, Environmental toxicology and chemistry.

[6]  M. Karnovsky,et al.  A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron-microscopy , 1965 .

[7]  Koichi Kato.  The osmium tetroxide procedure for light and electron microscopy of ABS plastics , 1967 .

[8]  V. Rotello,et al.  Effect of Surface Charge on the Uptake and Distribution of Gold Nanoparticles in Four Plant Species , 2012, Environmental science & technology.

[9]  J. Nagy,et al.  Respiratory toxicity of multi-wall carbon nanotubes. , 2005, Toxicology and applied pharmacology.

[10]  A. Spurr,et al.  ULTRASTRUCTURE OF CHLOROPLASTS AND CHROMOPLASTS IN CAPSICUM ANNUUM I. THYLAKOID MEMBRANE CHANGES DURING FRUIT RIPENING , 1968 .

[11]  Lenore L. Dai,et al.  Effects of functionalized and nonfunctionalized single‐walled carbon nanotubes on root elongation of select crop species , 2008, Environmental toxicology and chemistry.

[12]  R. Baughman,et al.  Carbon Nanotubes: Present and Future Commercial Applications , 2013, Science.

[13]  Guibin Jiang,et al.  Effective Surface Charge Density Determines the Electrostatic Attraction between Nanoparticles and Cells , 2012 .

[14]  Kevin C Jones,et al.  Novel method for the direct visualization of in vivo nanomaterials and chemical interactions in plants. , 2009, Environmental science & technology.

[15]  M. Prato,et al.  Biomedical applications of functionalised carbon nanotubes. , 2005, Chemical communications.

[16]  Yuliang Zhao,et al.  Cytotoxicity of carbon nanomaterials: single-wall nanotube, multi-wall nanotube, and fullerene. , 2005, Environmental science & technology.

[17]  Yi Zhang,et al.  Repeated carbon nanotube administrations in male mice cause reversible testis damage without affecting fertility , 2010, Nature Nanotechnology.

[18]  Alejandro Pérez-de-Luque,et al.  Nanoparticles as smart treatment-delivery systems in plants: assessment of different techniques of microscopy for their visualization in plant tissues. , 2008, Annals of botany.

[19]  Jose R Peralta-Videa,et al.  Interaction of nanoparticles with edible plants and their possible implications in the food chain. , 2011, Journal of agricultural and food chemistry.

[20]  J. Terzakis.  Uranyl acetate, a stain and a fixative. , 1968, Journal of ultrastructure research.

[21]  N. Bottini,et al.  Multi-walled carbon nanotubes induce T lymphocyte apoptosis. , 2006, Toxicology letters.

[22]  J. Schnoor,et al.  Uptake, translocation, and transformation of quantum dots with cationic versus anionic coatings by Populus deltoides × nigra cuttings. , 2014, Environmental science & technology.

[23]  A. Spurr.  A low-viscosity epoxy resin embedding medium for electron microscopy. , 1969, Journal of ultrastructure research.

[24]  B. Fugetsu,et al.  Multi-Walled Carbon Nanotubes Interact with Cultured Rice Cells: Evidence of a Self-Defense Response , 2007 .

[25]  M. Prato,et al.  Carbon nanotubes as nanomedicines: from toxicology to pharmacology. , 2006, Advanced drug delivery reviews.

[26]  Wei Wang,et al.  Advances toward bioapplications of carbon nanotubes , 2004 .

[27]  B. Fugetsu,et al.  Studies on toxicity of multi-walled carbon nanotubes on Arabidopsis T87 suspension cells. , 2009, Journal of hazardous materials.

[28]  Xiao Zhang,et al.  Biodistribution of functionalized multiwall carbon nanotubes in mice. , 2007, Nuclear medicine and biology.

[29]  A. Murphy,et al.  Endocytotic cycling of PM proteins. , 2005, Annual review of plant biology.

[30]  H. Dai,et al.  Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destruction. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[31]  Qian Hu,et al.  Uptake, translocation, and transmission of carbon nanomaterials in rice plants. , 2009, Small.

[32]  B. Fugetsu,et al.  Studies on toxicity of multi-walled carbon nanotubes on suspension rice cells , 2009 .

[33]  Baoshan Xing,et al.  Phytotoxicity of nanoparticles: inhibition of seed germination and root growth. , 2007, Environmental pollution.

[34]  A. Biris,et al.  Carbon nanotubes induce growth enhancement of tobacco cells. , 2012, ACS nano.

[35]  Xiaohong Fang,et al.  Carbon nanotubes as molecular transporters for walled plant cells. , 2009, Nano letters.

[36]  Driton Vllasaliu,et al.  Mechanisms of nanoparticle internalization and transport across an intestinal epithelial cell model: effect of size and surface charge. , 2014, Molecular pharmaceutics.

[37]  R. Nemanich,et al.  Multi-walled carbon nanotube interactions with human epidermal keratinocytes. , 2005, Toxicology letters.

[38]  A. Biris,et al.  Impact of carbon nanotube exposure to seeds of valuable crops. , 2013, ACS applied materials & interfaces.

[39]  R. Fischer,et al.  Uptake of a Fluorescent Marker in Plant Cells Is Sensitive to Brefeldin A and Wortmannin Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010339. , 2002, The Plant Cell Online.