Modelo experimental de reconstrução em falha mandibular em coelhos

This paper aims to present a new experimental model of mandibular defect reconstruction in rabbits. It was used 14 animals, in which, in a first stage the left permanent inferior incisive tooth was extracted surgically. After a 50 days period for the bone to fulfill the dentary alveolus, surgery was performed. At first, a 10x5x5mm autograft was removed form the iliac crest, following a partial mandibular failure in the bone with the same auto graft size which was filled with the graft and fixed using titanium microplate. The rabbits were submitted to clinical and radiographic evaluation and 7 of them were euthanasiated at 15 days and the other 7 at 30 days to macro and microscopic analysis. The water and commercial food ingestion was not compromised, and the animals did not showed any chewing or apprehension difficulties, neither pain. Only one animal presented rejection to one screw, without occurring micro plate nor auto graft dislocation. This method showed to be efficient for an experimental model of reconstruction in mandibular defects of rabbits, demonstrating a healing evolution of the graft through radiographic, macroscopic and microscopic exams in 15th and 30th days.

[1]  Paolo Giannoni,et al.  A tissue engineering approach to bone repair in large animal models and in clinical practice. , 2007, Biomaterials.

[2]  Lúcia de Noronha,et al.  Comparação entre os bioenxertos de hidroxiapatita de cálcio e submucosa de intestino delgado porcino no preenchimento de defeitos ósseos criados em mandíbula de ratos , 2006 .

[3]  D. Baur,et al.  Distraction osteogenesis in irradiated rabbit mandibles with adjunctive hyperbaric oxygen therapy. , 2006, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[4]  Zhi Li,et al.  Repair of mandible defect with tissue engineering bone in rabbits , 2005, ANZ journal of surgery.

[5]  F. Verstraete,et al.  Dentistry in pet rabbits , 2005 .

[6]  O. Arosarena,et al.  Bone Regeneration in the Rat Mandible with Bone Morphogenetic Protein-2: A Comparison of Two Carriers , 2005, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[7]  H. Bilgili,et al.  Treatment of fractures of the mandible and maxilla by mini titanium plate fixation systems in dogs and cats. , 2003, Australian veterinary journal.

[8]  K. Tominaga,et al.  Effects of latency period in a rabbit mandibular distraction osteogenesis. , 2003, International journal of oral and maxillofacial surgery.

[9]  L. Malmgren,et al.  Defect repair in the rat mandible with bone morphogenic proteins and marrow cells. , 2003, Archives of facial plastic surgery.

[10]  L. Rasmusson,et al.  An experimental rabbit model for studying the healing of onlay bone grafts. , 1998, Swedish Dental Journal.

[11]  L. Sennerby,et al.  An experimental rabbit model for jaw-bone healing. , 1997, International Journal of Oral & Maxillofacial Surgery.

[12]  K. Michi,et al.  An experimental study of healing around hydroxylapatite implants installed with autogenous iliac bone grafts for jaw reconstruction. , 1991, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[13]  P. E. Calonius,et al.  Transplantation of free tibial periosteal graft on alveolar bone defect in adult rabbit. , 1981, International journal of oral surgery.