Molecular characterization of human adenoviruses isolated in Italy.

There is little information on the epidemiology of Human Adenoviruses (HAdVs) in Italy. In this study, 103 HAdV isolates, collected by the A. Gemelli Hospital (Catholic University Medical School of Rome, Italy) between 1987 and 2005, were genotyped by sequencing and phylogenetic analysis on a partial hexon gene region. Nine different serotypes belonging to all six HAdV species were identified. Serotype 2 was the most frequent (53.4%), followed by serotype 1 (15.53%) and serotype 41 (9.7%). Partial-hexon-based identification was confirmed as an effective tool for studying the molecular epidemiology of HAdVs.

[1]  N. Okabe,et al.  Multiplex PCR Assay for Rapid Identification of Oculopathogenic Adenoviruses by Amplification of the Fiber and Hexon Genes , 2005, Journal of Clinical Microbiology.

[2]  N. Georgiadis,et al.  Molecular epidemiology of adenovirus strains isolated from patients with ocular disease in the area of Thessaloniki, Greece (1998–2002) , 2005, Journal of medical virology.

[3]  K. Ohgami,et al.  Five New Genome Types of Adenovirus Type 37 Caused Epidemic Keratoconjunctivitis in Sapporo, Japan, for More Than 10 Years , 2005, Journal of Clinical Microbiology.

[4]  Kentaro Okamoto,et al.  [Two cases of acute encephalitis/encephalopathy associated with adenovirus type 3 infection]. , 2004, No to hattatsu = Brain and development.

[5]  Martha J. Brown,et al.  Comprehensive Detection and Serotyping of Human Adenoviruses by PCR and Sequencing , 2004, Journal of Clinical Microbiology.

[6]  Ding-Ping Liu,et al.  A two decade survey of respiratory adenovirus in Taiwan: The reemergence of adenovirus types 7 and 4 , 2004, Journal of medical virology.

[7]  S. Ohno,et al.  Molecular Diagnosis of Human Adenoviruses D and E by a Phylogeny-Based Classification Method Using a Partial Hexon Sequence , 2004, Journal of Clinical Microbiology.

[8]  M. Sobsey,et al.  Detection of Infectious Adenovirus in Cell Culture by mRNA Reverse Transcription-PCR , 2003, Applied and Environmental Microbiology.

[9]  A. Heim,et al.  Rapid and quantitative detection of human adenovirus DNA by real‐time PCR , 2003, Journal of medical virology.

[10]  D. Vaughn,et al.  Large epidemic of adenovirus type 4 infection among military trainees: epidemiological, clinical, and laboratory studies. , 2002, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[11]  G. Gray,et al.  Large epidemic of respiratory illness due to adenovirus types 7 and 3 in healthy young adults. , 2002, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[12]  Tetsuya Yoshida,et al.  Molecular and Epidemiological Analyses of Human Adenovirus Type 7 Strains Isolated from the 1995 Nationwide Outbreak in Japan , 2002, Journal of Clinical Microbiology.

[13]  H. Ushijima,et al.  Rapid detection and typing of oculopathogenic strain of subgenus D adenoviruses by fiber-based PCR and restriction enzyme analysis. , 2001, Investigative ophthalmology & visual science.

[14]  B. Albinsson,et al.  Rapid Typing of Human Adenoviruses by a General PCR Combined with Restriction Endonuclease Analysis , 2001, Journal of Clinical Microbiology.

[15]  S. Zielen,et al.  Severe adenovirus bronchiolitis in children , 2000, Acta paediatrica.

[16]  D. Erdman,et al.  Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay , 2000, Journal of Clinical Microbiology.

[17]  G. Gray,et al.  Adult adenovirus infections: loss of orphaned vaccines precipitates military respiratory disease epidemics. For the Adenovirus Surveillance Group. , 2000, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[18]  S. Khoo,et al.  Adenoviruses from Human Immunodeficiency Virus-Infected Individuals, Including Two Strains That Represent New Candidate Serotypes Ad50 and Ad51 of Species B1 and D, Respectively , 1999, Journal of Clinical Microbiology.

[19]  G. Gray,et al.  Respiratory diseases among U.S. military personnel: countering emerging threats. , 1999, Emerging infectious diseases.

[20]  M. Lechevallier,et al.  A Strategy for Detection of Viruses in Groundwater by PCR , 1999, Applied and Environmental Microbiology.

[21]  P. Piedra,et al.  Disseminated adenovirus disease in immunocompromised and immunocompetent children. , 1998, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[22]  U. Bauer,et al.  Rapid identification of subgenera of human adenovirus by serological and PCR assays. , 1998, Journal of virological methods.

[23]  G. Wadell,et al.  Molecular epidemiology of adenovirus types 3 and 7 isolated from children with pneumonia in Beijing , 1996, Journal of medical virology.

[24]  J. D. de Jong,et al.  Rapid subgenus identification of human adenovirus isolates by a general PCR , 1996, Journal of clinical microbiology.

[25]  G. Wadell,et al.  Molecular epidemiology of adenovirus acute lower respiratory infections of children in the South Cone of South America (1991–1994) , 1996, Journal of medical virology.

[26]  A. Kidd,et al.  Characterization of a single SA7-like VA RNA gene in subgroup F adenoviruses. , 1993, The Journal of general virology.

[27]  A. Osterhaus,et al.  Detection, typing, and subtyping of enteric adenoviruses 40 and 41 from fecal samples and observation of changing incidences of infections with these types and subtypes , 1993, Journal of clinical microbiology.

[28]  R. Girones,et al.  Polymerase chain reaction for detection of adenoviruses in stool samples , 1991, Journal of clinical microbiology.

[29]  R. Wigand.  Pitfalls in the identification of adenoviruses. , 1987, Journal of virological methods.

[30]  P. E. Valensin,et al.  Viral childhood gastroenteritis. , 1986, Microbiologica.

[31]  G. Hammond,et al.  Examination of uncommon clinical isolates of human adenoviruses by restriction endonuclease analysis , 1985, Journal of clinical microbiology.

[32]  J. Obijeski,et al.  The changing etiology of epidemic keratoconjunctivitis: antigenic and restriction enzyme analyses of adenovirus types 19 and 37 isolated over a 10-year period. , 1983, The Journal of infectious diseases.

[33]  E. Buescher,et al.  Control of respiratory disease in recruits with types 4 and 7 adenovirus vaccines. , 1971, American journal of epidemiology.

[34]  H. M. Rose,et al.  Adenoviral infection in military recruits. , 1970, Archives of environmental health.

[35]  T. Adrian,et al.  Genome type analysis of adenovirus 31, a potential causative agent of infants' enteritis , 2005, Archives of Virology.

[36]  J. Broderson,et al.  Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera , 2005, Archives of Virology.

[37]  K. Aoki,et al.  A Twenty-One Year Surveillance of Adenoviral Conjunctivitis in Sapporo, Japan , 2002, International ophthalmology clinics.

[38]  P. Klapper,et al.  PCR and restriction endonuclease analysis for rapid identification of human adenovirus subgenera. , 2000, Journal of clinical microbiology.

[39]  K. Schleifer,et al.  Microbiological safety of drinking water. , 2000, Annual Review of Microbiology.

[40]  R. Räty,et al.  Efficacy of PCR and other diagnostic methods for the detection of respiratory adenoviral infections. , 1999, Journal of medical virology.

[41]  E. Mendelson,et al.  Molecular epidemiology of adenovirus type 7 in Israel: identification of two new genome types, Ad7k and Ad7d2. , 1998, Journal of medical virology.

[42]  M. Kobayashi,et al.  Epidemiology of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children in the Tokyo area. , 1991, Scandinavian journal of infectious diseases.

[43]  F. Rumpianesi,et al.  A two-year longitudinal study on the etiology of acute diarrhea in young children in Northern Italy. , 1985, Microbiologica.

[44]  G. Wadell.  Molecular epidemiology of human adenoviruses. , 1984, Current topics in microbiology and immunology.