Hyperspectral imaging (HSI) to evaluate the interaction of optically active nanoparticles in biological media and cells

Abstract. Hyperspectral Imaging (HSI) is a technique that can be used with darkfield microscopy for investigating biological interactions of optically active nanoparticles (NP) based on their spectral signatures. The objective of this study is to investigate the capabilities of HSI technology to characterize (1) spectral characteristics of gold, silver, and manganese NPs, (2) stability of gold NPs in exposure media and growth media based on spectral shifts, and (3) cellular interaction of NPs by correlating spectral data between NPs in water or media and NPs exposed to cells. Results demonstrate the unique spectral characteristics of the NPs investigated. Gold and silver NPs both display plasmonic properties; however, gold NPs exhibited a more narrow spectral profile. Silver NPs demonstrated several distinct scattering spectra due to the strong dependence on morphology. Manganese NPs do not display plasmonic properties, but still exhibited a unique spectral response, allowing them to be detected in cells. Due to the narrow spectral profile for gold NPs, stability was investigated in biological media, demonstrating the ability for HSI to detect the stabilizing effect of serum on NPs. Cellular interaction studies showed that the peak scattering wavelength for NPs in cells deviated from NPs in water or media, but NPs were still able to be identified based on intensity and shape of the scattering curves. Overall, HSI was demonstrated as a useful technique for evaluating optically active NPs in biological media and cellular environments.

[1]  Rebecca Richards-Kortum,et al.  Plasmonic nanosensors for imaging intracellular biomarkers in live cells. , 2007, Nano letters.

[2]  P. Kamat,et al.  What Factors Control the Size and Shape of Silver Nanoparticles in the Citrate Ion Reduction Method , 2004 .

[3]  B. Foster.  Focus on microscopy: A technique for imaging live cell interactions and mechanisms , 2004 .

[4]  John J. Schlager,et al.  Assessment of Metal Nanoparticle Agglomeration, Uptake, and Interaction Using High-Illuminating System , 2007, International journal of toxicology.

[5]  David R. Smith,et al.  Shape effects in plasmon resonance of individual colloidal silver nanoparticles , 2002 .

[6]  L. Dai,et al.  Preparation of cells for assessing ultrastructural localization of nanoparticles with transmission electron microscopy , 2010, Nature Protocols.

[7]  Andrew McCaskie,et al.  Nanomedicine , 2005, BMJ.

[8]  Ricardas Rotomskis,et al.  Interaction of Water-Soluble CdTe Quantum Dots with Bovine Serum Albumin , 2010, Nanoscale research letters.

[9]  S. Nie,et al.  Luminescent quantum dots for multiplexed biological detection and imaging. , 2002, Current opinion in biotechnology.

[10]  David R. Smith,et al.  Local Refractive Index Dependence of Plasmon Resonance Spectra from Individual Nanoparticles , 2003 .

[11]  Dmitri O. Lapotko,et al.  Methods for monitoring and imaging nanoparticles in cells , 2007, SPIE BiOS.

[12]  Betty Y. S. Kim,et al.  Current concepts: Nanomedicine , 2010 .

[13]  J. Yguerabide,et al.  Light-scattering submicroscopic particles as highly fluorescent analogs and their use as tracer labels in clinical and biological applications. , 1998, Analytical biochemistry.

[14]  Jerilyn A. Timlin,et al.  Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution , 2006, Journal of Neuroscience Methods.

[15]  Guido Viscardi,et al.  Highly bright and photostable cyanine dye-doped silica nanoparticles for optical imaging: Photophysical characterization and cell tests , 2010 .

[16]  V. Vodyanoy.  High Resolution Light Microscopy of Live Cells , 2005, Microscopy Today.

[17]  D. Balding,et al.  HLA Sequence Polymorphism and the Origin of Humans , 2006 .

[18]  S. Bhatia,et al.  Probing the Cytotoxicity Of Semiconductor Quantum Dots. , 2004, Nano letters.

[19]  A. Siddiqi,et al.  Use of hyperspectral imaging to distinguish normal, precancerous, and cancerous cells , 2008, Cancer.

[20]  Bengt Fadeel,et al.  Better safe than sorry: Understanding the toxicological properties of inorganic nanoparticles manufactured for biomedical applications. , 2010, Advanced drug delivery reviews.

[21]  J. Hoogenboom,et al.  Beyond quantum jumps: Blinking nanoscale light emitters , 2009 .

[22]  Kort Travis,et al.  Dynamic imaging of molecular assemblies in live cells based on nanoparticle plasmon resonance coupling. , 2009, Nano letters.

[23]  A. Alivisatos,et al.  Melting in Semiconductor Nanocrystals , 1992, Science.

[24]  John J. Schlager,et al.  Toxicity Evaluation for Safe Use of Nanomaterials: Recent Achievements and Technical Challenges , 2009 .

[25]  S. Aaronson,et al.  In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. , 1973, Journal of the National Cancer Institute.

[26]  Steffen Hackbarth,et al.  Long-term exposure to CdTe quantum dots causes functional impairments in live cells. , 2007, Langmuir : the ACS journal of surfaces and colloids.