Preparation of Anti-Aspergillus fumigatus Antibodies in Egg Laying Hens and Their Protective Efficacy in BALB/c Mice

The aim of this study was to prepare anti-Aspergillus fumigatus antibodies in egg-laying hens and test their protective efficacy in BALB/c mice. Heat-killed A fumigatus was emulsified with Freunds complete and incomplete adjuvant. Egglaying hens were injected subcutaneously with each emulsion, with a 15-day interval between the former and the latter preparation. IgY-rich extracts were obtained from the yolk of eggs laid preand post-immunization using the EGGstract IgY system (Promega), pooled, dialyzed, and lyophilized. The presence of IgY anti-A fumigatus antibodies in the post-immunization extracts was confirmed by ELISA. SDS-polyacrylamide gel electrophoresis (SDSPAGE) of 2-mercaptoethanol (2-ME)-treated post-immunization extract revealed heavy and light chain bands. A 190-KD band representing IgY was obtained when the extract that was not treated with 2-ME was subjected to

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