Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins.

Focal adhesion of leukocytes to the blood vessel lining is a key step in inflammation and certain vascular disease processes. Endothelial leukocyte adhesion molecule-1 (ELAM-1), a cell surface glycoprotein expressed by cytokine-activated endothelium, mediates the adhesion of blood neutrophils. A full-length complementary DNA (cDNA) for ELAM-1 has now been isolated by transient expression in COS cells. Cells transfected with the ELAM-1 clone express a surface structure recognized by two ELAM-1 specific monoclonal antibodies (H4/18 and H18/7) and support the adhesion of isolated human neutrophils and the promyelocytic cell line HL-60. Expression of ELAM-1 transcripts in cultured human endothelial cells is induced by cytokines, reaching a maximum at 2 to 4 hours and decaying by 24 hours; cell surface expression of ELAM-1 protein parallels that of the mRNA. The primary sequence of ELAM-1 predicts an amino-terminal lectin-like domain, an EGF domain, and six tandem repetitive motifs (about 60 amino acids each) related to those found in complement regulatory proteins. A similar domain structure is also found in the MEL-14 lymphocyte cell surface homing receptor, and in granule-membrane protein 140, a membrane glycoprotein of platelet and endothelial secretory granules that can be rapidly mobilized (less than 5 minutes) to the cell surface by thrombin and other stimuli. Thus, ELAM-1 may be a member of a nascent gene family of cell surface molecules involved in the regulation of inflammatory and immunological events at the interface of vessel wall and blood.

[1]  S. Licht,et al.  Time and Spatial Dependence of the Concentration of Less Than 105 Microelectrode-Generated Molecules , 1989, Science.

[2]  G. Weddell,et al.  Signal peptide for protein secretion directing glycophospholipid membrane anchor attachment. , 1989, Science.

[3]  D. Faller,et al.  Relative contribution of the leukocyte molecules MO1, LFA‐1, and p150,95 (LeuM5) in adhesion of granulocytes and monocytes to vascular endothelium is tissue‐ and stimulus‐specific , 1988, Journal of cellular physiology.

[4]  C W Smith,et al.  Recognition of an endothelial determinant for CD 18-dependent human neutrophil adherence and transendothelial migration. , 1988, The Journal of clinical investigation.

[5]  E. Butcher,et al.  Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes , 1988, The Journal of cell biology.

[6]  F. Luscinskas,et al.  Cultured human endothelial cells stimulated with cytokines or endotoxin produce an inhibitor of leukocyte adhesion. , 1988, The Journal of clinical investigation.

[7]  Michael Loran Dustin,et al.  Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells , 1988, The Journal of cell biology.

[8]  M. Cybulsky,et al.  Neutrophil leukocyte emigration induced by endotoxin. Mediator roles of interleukin 1 and tumor necrosis factor alpha 1. , 1988, Journal of immunology.

[9]  D. Fearon,et al.  Organization of the genes encoding complement receptors type 1 and 2, decay-accelerating factor, and C4-binding protein in the RCA locus on human chromosome 1 , 1988, The Journal of experimental medicine.

[10]  Michael Loran Dustin,et al.  Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families , 1988, Cell.

[11]  E. Chi,et al.  A monoclonal antibody to the adherence-promoting leukocyte glycoprotein, CD18, reduces organ injury and improves survival from hemorrhagic shock and resuscitation in rabbits. , 1988, The Journal of clinical investigation.

[12]  B. Seed,et al.  ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM , 1988, Nature.

[13]  P. J. Simpson,et al.  Reduction of experimental canine myocardial reperfusion injury by a monoclonal antibody (anti-Mo1, anti-CD11b) that inhibits leukocyte adhesion. , 1988, The Journal of clinical investigation.

[14]  Eugene C. Butcher,et al.  A tissue-specific endothelial cell molecule involved in lymphocyte homing , 1988, Nature.

[15]  A. F. Williams,et al.  The immunoglobulin superfamily--domains for cell surface recognition. , 1988, Annual review of immunology.

[16]  B. Seed,et al.  Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[17]  R. Cotran,et al.  Identification of an inducible endothelial-leukocyte adhesion molecule. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[18]  E Ruoslahti,et al.  New perspectives in cell adhesion: RGD and integrins. , 1987, Science.

[19]  E. Butcher,et al.  Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes. , 1987, Journal of immunology.

[20]  B. Seed,et al.  Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[21]  J. Zaia,et al.  Decay accelerating factor (DAF) peptide sequences share homology with a consensus sequence found in the superfamily of structurally related complement proteins and other proteins including haptoglobin, factor XIII, beta 2-glycoprotein I, and the IL-2 receptor. , 1987, Immunology letters.

[22]  D. Fearon,et al.  Human C3b/C4b receptor (CR1). Demonstration of long homologous repeating domains that are composed of the short consensus repeats characteristics of C3/C4 binding proteins , 1987, The Journal of experimental medicine.

[23]  E. Butcher,et al.  Receptors involved in lymphocyte homing: relationship between a carbohydrate-binding receptor and the MEL-14 antigen , 1987, The Journal of cell biology.

[24]  T. Yednock,et al.  Phosphomannosyl-derivatized beads detect a receptor involved in lymphocyte homing , 1987, The Journal of cell biology.

[25]  T. Springer,et al.  Cloning of the β subunit of the leukocyte adhesion proteins: Homology to an extracellular matrix receptor defines a novel supergene family , 1987, Cell.

[26]  Richard O. Hynes,et al.  Integrins: A family of cell surface receptors , 1987, Cell.

[27]  K. Drickamer,et al.  Exon structure of a mannose-binding protein gene reflects its evolutionary relationship to the asialoglycoprotein receptor and nonfibrillar collagens. , 1987, The Journal of biological chemistry.

[28]  T. Honjo,et al.  Human lymphocyte Fc receptor for IgE: sequence homology of its cloned cDNA with animal lectins. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[29]  C. Gahmberg,et al.  Identification of a novel adhesion molecule in human leukocytes by monoclonal antibody LB‐2 , 1987, FEBS letters.

[30]  I. Campbell,et al.  The solution structure of human epidermal growth factor , 1987, Nature.

[31]  T. Hirano,et al.  Molecular structure of human lymphocyte receptor for immunoglobulin E , 1986, Cell.

[32]  P. Berg,et al.  Formation of functional asialoglycoprotein receptor after transfection with cDNAs encoding the receptor proteins. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[33]  W. Fiers,et al.  Overlapping patterns of activation of human endothelial cells by interleukin 1, tumor necrosis factor, and immune interferon. , 1986, Journal of immunology.

[34]  G. Shaw,et al.  A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation , 1986, Cell.

[35]  R. Cotran,et al.  Induction and detection of a human endothelial activation antigen in vivo , 1986, The Journal of experimental medicine.

[36]  D. Bentley,et al.  Complement system proteins which interact with C3b or C4b A superfamily of structurally related proteins. , 1986, Immunology today.

[37]  Michael Loran Dustin,et al.  Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). , 1986, Journal of immunology.

[38]  H. Ochs,et al.  An endothelial cell surface factor(s) induced in vitro by lipopolysaccharide, interleukin 1, and tumor necrosis factor-alpha increases neutrophil adherence by a CDw18-dependent mechanism. , 1986, Journal of immunology.

[39]  G. Heijne.  A new method for predicting signal sequence cleavage sites. , 1986 .

[40]  W. Fiers,et al.  Two distinct monokines, interleukin 1 and tumor necrosis factor, each independently induce biosynthesis and transient expression of the same antigen on the surface of cultured human vascular endothelial cells. , 1986, Journal of immunology.

[41]  B. Beutler,et al.  Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[42]  I. Weissman,et al.  Cell surface molecule associated with lymphocyte homing is a ubiquitinated branched-chain glycoprotein. , 1986, Science.

[43]  D. Haskard,et al.  Interleukin 1 increases the binding of human B and T lymphocytes to endothelial cell monolayers. , 1986, Journal of immunology.

[44]  R. Schleimer,et al.  Cultured human vascular endothelial cells acquire adhesiveness for neutrophils after stimulation with interleukin 1, endotoxin, and tumor-promoting phorbol diesters. , 1986, Journal of immunology.

[45]  J. Gamble,et al.  Stimulation of the adherence of neutrophils to umbilical vein endothelium by human recombinant tumor necrosis factor. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[46]  R. Cotran,et al.  Interleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related leukocyte cell lines. , 1985, The Journal of clinical investigation.

[47]  H. Lodish,et al.  Sequence of a second human asialoglycoprotein receptor: conservation of two receptor genes during evolution. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[48]  M. Shuman,et al.  A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation , 1985, The Journal of cell biology.

[49]  L. Hood,et al.  T cell antigen receptors and the immunoglobulin supergene family , 1985, Cell.

[50]  R. McEver,et al.  A monoclonal antibody to a membrane glycoprotein binds only to activated platelets. , 1984, The Journal of biological chemistry.

[51]  B. Furie,et al.  A platelet membrane protein expressed during platelet activation and secretion. Studies using a monoclonal antibody specific for thrombin-activated platelets. , 1984, The Journal of biological chemistry.

[52]  M. Kozak.  Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. , 1984, Nucleic acids research.

[53]  K. Kurachi,et al.  Isolation and characterization of a cDNA coding for human factor IX. , 1982, Proceedings of the National Academy of Sciences of the United States of America.