Guanidine and heat sensitivity of foot-and-mouth disease virus (FMDV) strains

SUMMARY A study of the ability of 49 strains of FMD virus to replicate in BHK-21 monolayer cells maintained under a standard agar overlay containing 5·2 mM guanidine hydrochloride and to withstand heat inactivation at 54°C for 1 h showed that strains belonging to serotypes C, O and Asia 1 were generally more resistant to guanidine and heat stable than the SAT 1, 2 and 3 serotypes. The type A viruses as a whole occupied an intermediate position between these two groups. In vitro passage in BHK-21 cells influenced the guanidine sensitivity of 3(O, C and SAT 3) of the 7 FMD serotypes suggesting that this is not a stable genetic marker. Heat stability of the FMD viruses, however, did not change on passage, suggesting that this is a stable characteristic inherent in any homogeneous FMD virus population.

[1]  A. Nomoto.  [Replication of picornaviruses]. , 1982, Uirusu.

[2]  J. Booth,et al.  Microneutralization tests for serological typing and subtyping of foot-and-mouth disease virus strains , 1978, Journal of Hygiene.

[3]  F. Brown,et al.  An assessment by competition hybridization of the sequence homology between the RNAs of the seven serotypes of FMDV. , 1977, The Journal of general virology.

[4]  D. Mccahon,et al.  An extended genetic recombination map for foot-and-mouth diseases virus. , 1977, The Journal of general virology.

[5]  Pereira Hg.  Subtyping of foot-and-mouth disease virus. , 1976 .

[6]  H. Pereira.  Subtyping of foot-and-mouth disease virus. , 1976, Developments in biological standardization.

[7]  J. Mackenzie,et al.  Evidence for recombination between two different immunological types of foot-and-mouth disease virus. , 1975, The Australian journal of experimental biology and medical science.

[8]  W. R. Slade,et al.  A genetic recombination map of foot-and-mouth disease virus. , 1975, The Journal of general virology.

[9]  K. Cowan,et al.  Antibody response to viral antigens. , 1973, Advances in immunology.

[10]  C. Pringle.  Electrophoretic properties of foot-and-mouth disease virus strains and the selection of intra-strain mutants. , 1969, The Journal of general virology.

[11]  F. Brown,et al.  Effect of actinomycin D and guanidine on the formation of a ribonucleic acid polymerase induced by foot-and mouth-disease virus and on the replication of virus and viral ribonucleic acid. , 1969, The Biochemical journal.

[12]  F. Brown,et al.  A study of the kinetics of protein and RNA synthesis induced by foot-and-mouth disease virus. , 1966, Biochimica et biophysica acta.

[13]  F. Brown,et al.  The effect of heat on the structure of foot-and-mouth disease virus and the viral ribonucleic acid. , 1966, Biochimica et biophysica acta.

[14]  C. Pringle.  EVIDENCE OF GENETIC RECOMBINATION IN FOOT-AND-MOUTH DISEASE VIRUS. , 1965, Virology.

[15]  C. Pringle.  Inhibition of Multiplication of Foot-and-Mouth Disease Virus by Guanidine Hydrochloride , 1964, Nature.

[16]  H. L. Bachrach.  FOOT-AND-MOUTH DISEASE VIRUS: STRUCTURE AND MECHANISM OF DEGRADATION AS DEDUCED FROM ABSORBANCE-TEMPERATURE RELATIONSHIPS. , 1964, Journal of molecular biology.

[17]  R. Telling,et al.  Growth of a Cloned Strain of Hamster Kidney Cells in Suspended Cultures and their Susceptibility to the Virus of Foot-and-Mouth Disease , 1962, Nature.

[18]  G. Mowat,et al.  Growth of Foot-and-Mouth Disease Virus in a Fibroblastic Cell Line Derived from Hamster Kidneys , 1962, Nature.

[19]  H. L. Bachrach,et al.  Thermal-Resistant Populations of Foot-and-Mouth Disease Virus , 1960, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[20]  F. Brown,et al.  Application of agar gel precipitin tests to the study of the virus of foot-and-mouth disease. , 1958, Virology.