Baculovirus as versatile vectors for protein expression in insect and mammalian cells

Today, many thousands of recombinant proteins, ranging from cytosolic enzymes to membrane-bound proteins, have been successfully produced in baculovirus-infected insect cells. Yet, in addition to its value in producing recombinant proteins in insect cells and larvae, this viral vector system continues to evolve in new and unexpected ways. This is exemplified by the development of engineered insect cell lines to mimic mammalian cell glycosylation of expressed proteins, baculovirus display strategies and the application of the virus as a mammalian-cell gene delivery vector. Novel vector design and cell engineering approaches will serve to further enhance the value of baculovirus technology.

[1]  D. Jarvis,et al.  Glycoproteins from Insect Cells: Sialylated or Not? , 2001, Biological chemistry.

[2]  M. Betenbaugh,et al.  Humanization of lepidopteran insect-cell-produced glycoproteins. , 2003, Accounts of chemical research.

[3]  P. Kitts,et al.  Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. , 1990, Nucleic acids research.

[4]  R. Ames,et al.  Heterologous expression of G protein-coupled receptors in U-2 OS osteosarcoma cells. , 2004, Receptors & channels.

[5]  M. Strauss,et al.  Baculovirus-mediated gene transfer in the presence of human serum or blood facilitated by inhibition of the complement system , 1998, Gene Therapy.

[6]  Shiaw-Min Hwang,et al.  Transgene expression and differentiation of baculovirus‐transduced human mesenchymal stem cells , 2005, The journal of gene medicine.

[7]  J. Hollister,et al.  Engineering the protein N-glycosylation pathway in insect cells for production of biantennary, complex N-glycans. , 2002, Biochemistry.

[8]  M. Stanley.  Progress in prophylactic and therapeutic vaccines for human papillomavirus infection , 2003, Expert review of vaccines.

[9]  A. Milon,et al.  Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification , 2003, Cellular and Molecular Life Sciences CMLS.

[10]  D. Jarvis,et al.  Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production. , 2003, Virology.

[11]  J. Hollister,et al.  Stable expression of mammalian beta 1,4-galactosyltransferase extends the N-glycosylation pathway in insect cells. , 1998, Glycobiology.

[12]  K. Airenne,et al.  Baculovirus display strategies: Emerging tools for eukaryotic libraries and gene delivery. , 2003, Briefings in functional genomics & proteomics.

[13]  K. Fujii,et al.  Co-expression of human chaperone Hsp70 and Hsdj or Hsp40 co-factor increases solubility of overexpressed target proteins in insect cells. , 2000, Biochimica et biophysica acta.

[14]  Yu-Chen Hu,et al.  Highly efficient baculovirus-mediated gene transfer into rat chondrocytes. , 2004, Biotechnology and bioengineering.

[15]  J. Barnard,et al.  Kinetic Analysis of Wild-Type and YMDD Mutant Hepatitis B Virus Polymerases and Effects of Deoxyribonucleotide Concentrations on Polymerase Activity , 2002, Antimicrobial Agents and Chemotherapy.

[16]  P. Roy,et al.  Virus-like particles as immunogens. , 2003, Trends in microbiology.

[17]  D. Wong,et al.  Hepatitis C Virus Structural Proteins Assemble into Viruslike Particles in Insect Cells , 1998, Journal of Virology.

[18]  F. Boyce,et al.  Combination treatment for osteosarcoma with baculoviral vector mediated gene therapy (p53) and chemotherapy (adriamycin) , 2001, Experimental & Molecular Medicine.

[19]  T. Rushmore,et al.  Bioreactor systems in drug metabolism: synthesis of cytochrome P450-generated metabolites. , 2000, Metabolic engineering.

[20]  M. Summers,et al.  Trends in the Development of Baculovirus Expression Vectors , 1988, Bio/Technology.

[21]  D. Jarvis,et al.  Use of Early Baculovirus Promoters for Continuous Expression and Efficient Processing of Foreign Gene Products in Stably Transformed Lepidopteran Cells , 1990, Bio/Technology.

[22]  A. Kamen,et al.  Quantitation of baculovirus particles by flow cytometry. , 2002, Journal of virological methods.

[23]  D. Massotte.  G protein-coupled receptor overexpression with the baculovirus-insect cell system: a tool for structural and functional studies. , 2003, Biochimica et biophysica acta.

[24]  G. de Thé,et al.  An HTLV-I/II vaccine: from animal models to clinical trials? , 1996, Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association.

[25]  A. Takada,et al.  Induction of antibodies in mice by a recombinant baculovirus expressing pseudorabies virus glycoprotein B in mammalian cells. , 1999, Veterinary microbiology.

[26]  H. Isom,et al.  Rebound of Hepatitis B Virus Replication in HepG2 Cells after Cessation of Antiviral Treatment , 2002, Journal of Virology.

[27]  I. L. Le Poole,et al.  HPV protein/peptide vaccines: from animal models to clinical trials. , 2003, Frontiers in bioscience : a journal and virtual library.

[28]  Yu-Chen Hu,et al.  Investigation of optimal transduction conditions for baculovirus‐mediated gene delivery into mammalian cells , 2004, Biotechnology and bioengineering.

[29]  K. Ishii,et al.  A hybrid baculovirus-T7 RNA polymerase system for recovery of an infectious virus from cDNA. , 1997, Virology.

[30]  M. Furusawa,et al.  Production of human alpha-interferon in silkworm using a baculovirus vector. , 1985, Nature.

[31]  Chari D Smith,et al.  Development of a Novel High-Throughput Surrogate Assay to Measure HIV Envelope/CCR5/CD4-Mediated Viral/Cell Fusion Using BacMam Baculovirus Technology , 2003, Journal of biomolecular screening.

[32]  T. Kodama,et al.  Selective Reconstitution and Recovery of Functional γ-Secretase Complex on Budded Baculovirus Particles*[boxs] , 2004, Journal of Biological Chemistry.

[33]  Thomas Kost,et al.  BacMam recombinant baculoviruses in G protein-coupled receptor drug discovery. , 2004, Receptors & channels.

[34]  R. Possee.  Baculoviruses as expression vectors. , 1997, Current opinion in biotechnology.

[35]  S. Jesuthasan,et al.  Baculovirus-Mediated Gene Expression in Zebrafish , 2003, Marine Biotechnology.

[36]  Masashi Takahashi,et al.  Establishment of a large-scale purification procedure for purified recombinant bovine interferon-tau produced by a silkworm-baculovirus gene expression system. , 2004, The Journal of veterinary medical science.

[37]  G. Devauchelle,et al.  Effect of the 5' non-translated region on self-assembly of hepatitis C virus genotype 1a structural proteins produced in insect cells. , 2004, The Journal of general virology.

[38]  F. Boyce,et al.  Baculovirus-mediated gene transfer into mammalian cells. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[39]  Daron G Ferris,et al.  Efficacy of a bivalent L1 virus-like particle vaccine in prevention of infection with human papillomavirus types 16 and 18 in young women: a randomised controlled trial , 2004, The Lancet.

[40]  P. Roy,et al.  Efficient assembly and release of SARS coronavirus‐like particles by a heterologous expression system , 2004, FEBS Letters.

[41]  S. Hood,et al.  Expression of cytochromes P450 in a baculovirus system. , 1998, Methods in molecular biology.

[42]  R. Garcea,et al.  Virus-like particles as vaccines and vessels for the delivery of small molecules. , 2004, Current opinion in biotechnology.

[43]  Y. Chao,et al.  Rapid Titer Determination of Baculovirus by Quantitative Real‐Time Polymerase Chain Reaction , 2008, Biotechnology progress.

[44]  Masayoshi Onishi,et al.  In Vitro and In Vivo Gene Delivery by Recombinant Baculoviruses , 2003, Journal of Virology.

[45]  Thomas A Kost,et al.  Recombinant baculoviruses as mammalian cell gene-delivery vectors. , 2002, Trends in biotechnology.

[46]  B. Roizman,et al.  The Domains of Glycoprotein D Required To Block Apoptosis Induced by Herpes Simplex Virus 1 Are Largely Distinct from Those Involved in Cell-Cell Fusion and Binding to Nectin1 , 2003, Journal of Virology.

[47]  A. Facciabene,et al.  Baculovirus Vectors Elicit Antigen-Specific Immune Responses in Mice , 2004, Journal of Virology.

[48]  Tatsuo Fukagawa,et al.  Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system. , 2005, Biochemical and biophysical research communications.

[49]  E. Park,et al.  Development of an Antibody‐Based Assay for Determination of Baculovirus Titers in 10 Hours , 2002, Biotechnology progress.

[50]  B. Yao,et al.  A constitutive super-enhancer: homologous region 3 of Bombyx mori nucleopolyhedrovirus. , 2004, Biochemical and biophysical research communications.

[51]  M. Arthur,et al.  High efficiency gene transfer into cultured primary rat and human hepatic stellate cells using baculovirus vectors. , 2002, Liver.

[52]  M. Marschall,et al.  Efficient and tightly regulated expression systems for the human cytomegalovirus major transactivator protein IE2p86 in permissive cells. , 2002, Virus research.

[53]  A. Strosberg,et al.  Recovery of homogeneous and functional β2-adrenergic receptors from extracellular baculovirus particles , 1997, Nature Biotechnology.

[54]  Varpu Marjomäki,et al.  Baculovirus capsid display: a novel tool for transduction imaging. , 2003, Molecular therapy : the journal of the American Society of Gene Therapy.

[55]  D. Rowlands,et al.  Efficient delivery and regulable expression of hepatitis C virus full-length and minigenome constructs in hepatocyte-derived cell lines using baculovirus vectors. , 2002, The Journal of general virology.

[56]  R. Vallee,et al.  The Herpes Simplex Virus 1 UL34 Protein Interacts with a Cytoplasmic Dynein Intermediate Chain and Targets Nuclear Membrane , 2000, Journal of Virology.

[57]  Yu-Chen Hu,et al.  Enhancement and Prolongation of Baculovirus‐Mediated Expression in Mammalian Cells: Focuses on Strategic Infection and Feeding , 2003, Biotechnology progress.

[58]  T. Richmond,et al.  Baculovirus expression system for heterologous multiprotein complexes , 2004, Nature Biotechnology.

[59]  B. Roizman,et al.  Herpes simplex virus protein kinase US3 activates and functionally overlaps protein kinase A to block apoptosis. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[60]  C. Tate,et al.  Calnexin co-expression and the use of weaker promoters increase the expression of correctly assembled Shaker potassium channel in insect cells. , 2003, Biochimica et biophysica acta.

[61]  R. Kotin,et al.  Insect cells as a factory to produce adeno-associated virus type 2 vectors. , 2002, Human gene therapy.

[62]  U. Yasar,et al.  Role of polymorphic human CYP2B6 in cyclophosphamide bioactivation , 2003, The Pharmacogenomics Journal.

[63]  O. Laitinen,et al.  A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo , 2005, Nucleic acids research.

[64]  M. Strauss,et al.  Protection of Baculovirus-Vectors against Complement-Mediated Inactivation by Recombinant Soluble Complement Receptor Type 1 , 1999, Biological chemistry.

[65]  J. Hollister,et al.  Engineering N-glycosylation pathways in the baculovirus-insect cell system. , 1998, Current opinion in biotechnology.

[66]  H. Isom,et al.  Use of the Hepatitis B Virus Recombinant Baculovirus-HepG2 System to Study the Effects of (−)-β-2′,3′-Dideoxy-3′-Thiacytidine on Replication of Hepatitis B Virus and Accumulation of Covalently Closed Circular DNA , 1999, Antimicrobial Agents and Chemotherapy.

[67]  J. Auwerx,et al.  The generation of monoclonal antibodies against human peroxisome proliferator-activated receptors (PPARs). , 2002, Journal of atherosclerosis and thrombosis.

[68]  J Barsoum,et al.  Efficient transduction of mammalian cells by a recombinant baculovirus having the vesicular stomatitis virus G glycoprotein. , 1997, Human gene therapy.

[69]  P. McAllister,et al.  Rapid expression of recombinant proteins in modified CHO cells using the baculovirus system , 2004, Cytotechnology.

[70]  J. P. Condreay,et al.  Titration of KATP channel expression in mammalian cells utilizing recombinant baculovirus transduction. , 2002, Receptors & channels.

[71]  Y. Maéda,et al.  Enhancement of protein expression in insect cells by a lobster tropomyosin cDNA leader sequence , 2002, FEBS letters.

[72]  P. Schlag,et al.  Efficient gene transfer into human hepatocytes by baculovirus vectors. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[73]  E. Cable,et al.  Role of paracellular junction complexes in baculovirus-mediated gene transfer to nondividing rat hepatocytes , 2003, Gene Therapy.

[74]  EphrinB2a in the zebrafish retinotectal system. , 2004, Journal of neurobiology.

[75]  P. van Endert,et al.  A chaperone-assisted high yield system for the production of HLA-DR4 tetramers in insect cells. , 2004, Journal of immunological methods.

[76]  S. Nickolls,et al.  Functional coupling of the human dopamine D2 receptor with G alpha i1, G alpha i2, G alpha i3 and G alpha o G proteins: evidence for agonist regulation of G protein selectivity. , 2003, British journal of pharmacology.

[77]  C. Plumpton,et al.  A cell-based time-resolved fluorescence assay for selection of antibody reagents for G protein-coupled receptor immunohistochemistry. , 2004, Journal of immunological methods.

[78]  J. Vlak,et al.  Development of a chitinase and v-cathepsin negative bacmid for improved integrity of secreted recombinant proteins. , 2004, Journal of virological methods.

[79]  J. Leong,et al.  Transduction of cultured fish cells with recombinant baculoviruses. , 2003, The Journal of general virology.

[80]  V C Emery,et al.  Baculovirus expression vectors. , 1991, Methods in molecular biology.

[81]  L. Nicholson,et al.  RNA interference mediated in human primary cells via recombinant baculoviral vectors. , 2005, Molecular therapy : the journal of the American Society of Gene Therapy.

[82]  T. Stamminger,et al.  Synergistic induction of intercellular adhesion molecule-1 by the human cytomegalovirus transactivators IE2p86 and pp71 is mediated via an Sp1-binding site. , 2003, The Journal of general virology.

[83]  Y. Schneider,et al.  Insect cell culture for industrial production of recombinant proteins , 2003, Applied Microbiology and Biotechnology.

[84]  Jared J. Aumiller,et al.  A transgenic insect cell line engineered to produce CMP-sialic acid and sialylated glycoproteins. , 2003, Glycobiology.

[85]  W. C. Clay,et al.  Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[86]  K. A. Harrap,et al.  Insect viruses , 1977, Nature.

[87]  B. Roizman,et al.  Glycoprotein D or J Delivered in transBlocks Apoptosis in SK-N-SH Cells Induced by a Herpes Simplex Virus 1 Mutant Lacking Intact Genes Expressing Both Glycoproteins , 2000, Journal of Virology.

[88]  J. Bilello,et al.  Transient Disruption of Intercellular Junctions Enables Baculovirus Entry into Nondividing Hepatocytes , 2001, Journal of Virology.

[89]  J. Fulghum,et al.  High-throughput screening for soluble recombinant expressed kinases in Escherichia coli and insect cells. , 2004, Protein expression and purification.

[90]  J. Vlak,et al.  Spontaneous excision of BAC vector sequences from bacmid-derived baculovirus expression vectors upon passage in insect cells. , 2003, The Journal of general virology.

[91]  S. Geisse,et al.  Optimisation of insect cell growth in deep-well blocks: development of a high-throughput insect cell expression screen. , 2005, Protein expression and purification.

[92]  B. Roizman,et al.  Truncated Forms of Glycoprotein D of Herpes Simplex Virus 1 Capable of Blocking Apoptosis and of Low-Efficiency Entry into Cells Form a Heterodimer Dependent on the Presence of a Cysteine Located in the Shared Transmembrane Domains , 2002, Journal of Virology.

[93]  P. Cruz,et al.  Production of core and virus-like particles with baculovirus infected insect cells. , 2002, Advances in biochemical engineering/biotechnology.

[94]  D. Jarvis,et al.  Immediate-early baculovirus vectors for foreign gene expression in transformed or infected insect cells. , 1996, Protein expression and purification.

[95]  T. Yokoyama,et al.  Expression of biologically active recombinant equine interferon-gamma by two different baculovirus gene expression systems using insect cells and silkworm larvae. , 2002, Cytokine.

[96]  J. Hollister,et al.  Evidence for a sialic acid salvaging pathway in lepidopteran insect cells. , 2003, Glycobiology.

[97]  J. Hollister,et al.  Engineering lepidopteran insect cells for sialoglycoprotein production by genetic transformation with mammalian β1,4-galactosyltransferase and α2,6-sialyltransferase genes , 2001 .

[98]  P. Lu,et al.  CYTOCHROME P450 3A4 IS THE MAJOR ENZYME INVOLVED IN THE METABOLISM OF THE SUBSTANCE P RECEPTOR ANTAGONIST APREPITANT , 2004, Drug Metabolism and Disposition.

[99]  M. Sanchez-Cespedes,et al.  An efficient expression system for the production of functionally active human LKB1. , 2005, Journal of biotechnology.

[100]  C. Y. Liu,et al.  Assembly of human severe acute respiratory syndrome coronavirus-like particles , 2004, Biochemical and Biophysical Research Communications.

[101]  D. James,et al.  Constraints on the transport and glycosylation of recombinant IFN-gamma in Chinese hamster ovary and insect cells. , 1999, Biotechnology and bioengineering.

[102]  C. Fipaldini,et al.  Expression of hepatitis C virus cDNA in human hepatoma cell line mediated by a hybrid baculovirus-HCV vector. , 1999, Virology.

[103]  Daron G Ferris,et al.  Efficacy of a bivalent L 1 virus-like particle vaccine in prevention of infection with human papillomavirus types 16 and 18 in young women : a randomised controlled trial , 2005 .

[104]  Y. Mori,et al.  Expression and one-step purification of bovine interleukin-21 (IL-21) in silkworms using a hybrid baculovirus expression system , 2004, Biotechnology Letters.

[105]  H. Thomas,et al.  Complement inhibition rescued mice allowing observation of transgene expression following intraportal delivery of baculovirus in mice , 2005, The journal of gene medicine.

[106]  S. Jameel,et al.  Purification and diagnostic utility of a recombinant hepatitis E virus capsid protein expressed in insect larvae. , 2003, Protein expression and purification.

[107]  D. Hedin,et al.  A Flow Cytometric Assay for Rapid, Accurate Determination of Baculovirus Titers , 2004 .

[108]  J. Stapleton,et al.  Recombinant hepatitis C virus‐like particles expressed by baculovirus: Utility in cell‐binding and antibody detection assays , 2002, Journal of medical virology.

[109]  O. Taboga,et al.  Immunological properties of FMDV-gP64 fusion proteins expressed on SF9 cell and baculovirus surfaces. , 2004, Vaccine.

[110]  M. Betenbaugh,et al.  Comparing N-glycan processing in mammalian cell lines to native and engineered lepidopteran insect cell lines , 2004, Glycoconjugate Journal.

[111]  K. Gopinathan,et al.  Baculovirus display of fusion protein of Peste des petits ruminants virus and hemagglutination protein of Rinderpest virus and immunogenicity of the displayed proteins in mouse model. , 2003, Virology.

[112]  Kyu-Jin Park,et al.  Hepatitis C virus core protein is efficiently released into the culture medium in insect cells. , 2004, Journal of biochemistry and molecular biology.

[113]  O. Laitinen,et al.  Improved generation of recombinant baculovirus genomes in Escherichia coli. , 2003, Nucleic acids research.

[114]  J. P. Condreay,et al.  Recombinant baculoviruses as expression vectors for insect and mammalian cells. , 1999, Current opinion in biotechnology.

[115]  C. Liang,et al.  High efficiency gene transfer into mammalian kidney cells using baculovirus vectors , 2003, Archives of Virology.

[116]  M. Furusawa,et al.  Production of human α-interferon in silkworm using a baculovirus vector , 1985, Nature.

[117]  M. Betenbaugh,et al.  Modifying secretion and post-translational processing in insect cells. , 1999, Current opinion in biotechnology.

[118]  Someet Narang,et al.  Biosynthesis of human-type N-glycans in heterologous systems. , 2004, Current opinion in structural biology.

[119]  R. Stevens,et al.  Scalable high-throughput micro-expression device for recombinant proteins. , 2004, BioTechniques.

[120]  T. Lee,et al.  Enhancement of correct protein folding in vivo by a non-lytic baculovirus. , 2004, The Biochemical journal.

[121]  S. Hasnain,et al.  The Homologous Region Sequence (hr1) of Autographa californica Multinucleocapsid Polyhedrosis Virus Can Enhance Transcription from Non-baculoviral Promoters in Mammalian Cells* , 2003, Journal of Biological Chemistry.

[122]  P. Kitts,et al.  An immunological assay for determination of baculovirus titers in 48 hours. , 1999, Analytical biochemistry.

[123]  Thomas A. Kost,et al.  Biosafety and Viral Gene Transfer Vectors , 2006 .

[124]  Sun U. Song,et al.  Effective transduction of osteogenic sarcoma cells by a baculovirus vector. , 2003, The Journal of general virology.

[125]  A. Gent.  Efficient Generation of Infectious Recombinant Baculoviruses , 2022 .

[126]  Shu Wang,et al.  Axonal transport of recombinant baculovirus vectors. , 2004, Molecular therapy : the journal of the American Society of Gene Therapy.

[127]  R. Katso,et al.  The application of BacMam technology in nuclear receptor drug discovery. , 2005, Biotechnology annual review.

[128]  C. Tate,et al.  Calreticulin Promotes Folding/Dimerization of Human Lipoprotein Lipase Expressed in Insect Cells (Sf21)* , 2003, Journal of Biological Chemistry.

[129]  P. Kitts,et al.  A method for producing recombinant baculovirus expression vectors at high frequency. , 1993, BioTechniques.

[130]  D. O'reilly,et al.  Baculovirus expression vectors: a laboratory manual. , 1992 .

[131]  L. Moore,et al.  Recombinant baculoviruses used to study estrogen receptor function in human osteosarcoma cells. , 2003, Assay and drug development technologies.

[132]  Varpu Marjomäki,et al.  Enhanced gene delivery by avidin-displaying baculovirus. , 2004, Molecular therapy : the journal of the American Society of Gene Therapy.

[133]  G. B. Wisely,et al.  Production of monoclonal antibodies using recombinant baculovirus displaying gp64-fusion proteins. , 2000, Journal of immunological methods.

[134]  K. Ishii,et al.  Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors. , 1997, The Journal of general virology.

[135]  K. Airenne,et al.  Baculoviruses exhibit restricted cell type specificity in rat brain: a comparison of baculovirus- and adenovirus-mediated intracerebral gene transfer in vivo , 2002, Gene Therapy.

[136]  M. Betenbaugh,et al.  Determination of nucleotides and sugar nucleotides involved in protein glycosylation by high-performance anion-exchange chromatography: sugar nucleotide contents in cultured insect cells and mammalian cells. , 2001, Analytical biochemistry.

[137]  S. Nickolls,et al.  Functional coupling of the human dopamine D2 receptor with Gαi1, Gαi2, Gαi3 and Gαo G proteins: evidence for agonist regulation of G protein selectivity , 2003 .

[138]  Zongyi Hu,et al.  Immunization with Hepatitis C Virus-Like Particles Induces Humoral and Cellular Immune Responses in Nonhuman Primates , 2004, Journal of Virology.

[139]  Y. Matsuura,et al.  Baculovirus Induces an Innate Immune Response and Confers Protection from Lethal Influenza Virus Infection in Mice1 , 2003, The Journal of Immunology.

[140]  J. P. Condreay,et al.  Influence of promoter choice and trichostatin A treatment on expression of baculovirus delivered genes in mammalian cells. , 2004, Protein expression and purification.

[141]  I. Jones,et al.  Improving baculovirus recombination. , 2003, Nucleic acids research.

[142]  F. Altmann,et al.  Chapter 10 Protein Glycosylation in Insects , 1995 .

[143]  H. Isom,et al.  Hepatitis B virus replication in human HepG2 cells mediated by hepatitis B virus recombinant baculovirus , 1998, Hepatology.

[144]  D. Rowlands,et al.  Introduction of replication-competent hepatitis C virus transcripts using a tetracycline-regulable baculovirus delivery system. , 2004, The Journal of general virology.

[145]  V. Nene,et al.  Fusion to green fluorescent protein improves expression levels of Theileria parva sporozoite surface antigen p67 in insect cells , 2002, Parasitology.

[146]  H. Petry,et al.  The use of virus-like particles for gene transfer. , 2003, Current opinion in molecular therapeutics.

[147]  T. Kodama,et al.  A Combinatorial G Protein-coupled Receptor Reconstitution System on Budded Baculovirus , 2003, Journal of Biological Chemistry.

[148]  Ian M. Jones,et al.  Eukaryotic Virus Display: Engineering the Major Surface Glycoprotein of the Autographa californica Nuclear Polyhedrosis Virus (AcNPV) for the Presentation of Foreign Proteins on the Virus Surface , 1995, Bio/Technology.

[149]  F. Altmann,et al.  Insect Cells Contain an Unusual, Membrane-bound -N-Acetylglucosaminidase Probably Involved in the Processing of Protein N-Glycans (*) , 1995, The Journal of Biological Chemistry.

[150]  R. Schreiber,et al.  Baculovirus Stimulates Antiviral Effects in Mammalian Cells , 1999, Journal of Virology.

[151]  S. Yoshida,et al.  Baculovirus virions displaying Plasmodium berghei circumsporozoite protein protect mice against malaria sporozoite infection. , 2003, Virology.

[152]  J. Vlak,et al.  Autographa californica baculoviruses with large genomic deletions are rapidly generated in infected insect cells. , 2001, Virology.