Lens culinaris lectin immobilized on sepharose: binding and sugar-specific release of intact tissue culture cells

Lens culinaris lectin (LCL) covalently linked to 2B Sepharose binds tissue culture cells to the matrix. This is prevented by hapten sugars specific for LCL. Unlike other immobilized lectins, lens culinaris lectin allows the removal of bound cells from the matrix on addition of the specific sugars in a concentration-dependent manner. Binding and release occur under physiological conditions. Released cells continue to grow.

[1]  J. Labat-robert.  Concanavalin A as a tool, H. Bittiger, H.P. Schnebi (Eds.). John Wiley and Sons Ltd (1976) , 1977 .

[2]  P. Cuatrecasas,et al.  A simplified method for cyanogen bromide activation of agarose for affinity chromatography. , 1974, Analytical biochemistry.

[3]  M. Fukuda,et al.  Chemical nature of the receptor site for various phytomitogens. , 1972, Biochemistry.

[4]  L. Sachs,et al.  Differences in the binding of fluorescent concanavalin A to the surface membrane of normal and transformed cells. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[5]  M. Crumpton,et al.  Isolation of glycoproteins from pig lymphocyte plasma membrane using Lens culinaris phytohemagglutinin. , 1972, Biochemical and biophysical research communications.

[6]  H. Sage,et al.  Studies on a phytohemagglutinin from the lentil: IV. Direct binding studies of Lens culinaris hemagglutinin with simple saccharides , 1971 .

[7]  G. Edelman,et al.  Cell fractionation and arrangement on fibers, beads, and surfaces. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[8]  L. Sachs,et al.  Interaction of the carbohydrate-binding protein concanavalin A with normal and transformed cells. , 1969, Proceedings of the National Academy of Sciences of the United States of America.

[9]  M. Burger,et al.  Identification of a tumor-specific determinant on neoplastic cell surfaces. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[10]  J. Aub,et al.  REACTIONS OF NORMAL AND TUMOR CELL SURFACES TO ENZYMES. I. WHEAT-GERM LIPASE AND ASSOCIATED MUCOPOLYSACCHARIDES. , 1963, Proceedings of the National Academy of Sciences of the United States of America.

[11]  E. J. Ambrose,et al.  The inhibition of tumour growth by enzymes in tissue culture. , 1961, Experimental cell research.

[12]  M. Burger,et al.  Cyclic membrane changes in animal cells: transformed cells permanently display a surface architecture detected in normal cells only during mitosis. , 1971, Proceedings of the National Academy of Sciences of the United States of America.