Compounds leached from quinoa seeds inhibit matrix metalloproteinase activity and intracellular reactive oxygen species

Quinoa (Chenopodium quinoa Willd.) is a seed crop rich in bioactive compounds including phytoecdysones (especially 20‐hydroxyecdysone, 20HE), polyphenols, proteins and essential fatty acids. We previously reported a method to leach and concentrate quinoa bioactives into a complex phytochemical mixture termed quinoa leachate (QL). Here, we aimed to determine the effect of QL and its chemically distinct fractions on five biochemical endpoints relevant to skin care applications: (i) cell viability, (ii) matrix metalloproteinase (MMP) mRNA expression, (iii) MMP enzymatic activity, (iv) tyrosinase enzymatic activity and (v) intracellular reactive oxygen species (ROS) production.

[1]  Brittany L. Graf,et al.  Quinoa seeds leach phytoecdysteroids and other compounds with anti-diabetic properties. , 2014, Food chemistry.

[2]  D. Fisher,et al.  UV signaling pathways within the skin , 2014, The Journal of investigative dermatology.

[3]  Z. Draelos,et al.  Dyspigmentation, skin physiology, and a novel approach to skin lightening , 2013, Journal of cosmetic dermatology.

[4]  M. Lila,et al.  Chemical and in vitro assessment of Alaskan coastal vegetation antioxidant capacity. , 2013, Journal of agricultural and food chemistry.

[5]  E. Gilbert,et al.  Recent advances in understanding the anti-diabetic actions of dietary flavonoids. , 2013, The Journal of nutritional biochemistry.

[6]  Brittany L. Graf,et al.  Role of Anthocyanins in Skin Aging and UV- Induced Skin Damage , 2013 .

[7]  I. Raskin,et al.  Bioactive polyphenols from muscadine grape and blackcurrant stably concentrated onto protein‐rich matrices for topical applications , 2013, International journal of cosmetic science.

[8]  É. Dupont,et al.  Beyond UV radiation: A skin under challenge , 2013, International journal of cosmetic science.

[9]  R. Watson,et al.  The Role of Polyphenols in Skin Health , 2013 .

[10]  R. Watson,et al.  Bioactive Dietary Factors and Plant Extracts in Dermatology , 2013 .

[11]  Jun Hu,et al.  20-Hydroxyecdysone Protects against Oxidative Stress-Induced Neuronal Injury by Scavenging Free Radicals and Modulating NF-κB and JNK Pathways , 2012, PloS one.

[12]  J. Blumberg,et al.  Flavonoid Basics: Chemistry, Sources, Mechanisms of Action, and Safety , 2012, Journal of nutrition in gerontology and geriatrics.

[13]  Luca Tiano,et al.  Reference gene validation for qPCR on normoxia- and hypoxia-cultured human dermal fibroblasts exposed to UVA: is β-actin a reliable normalizer for photoaging studies? , 2011, Journal of biotechnology.

[14]  C. Heard,et al.  Topically applied KTTKS: a review , 2011, International journal of cosmetic science.

[15]  M. Landthaler,et al.  Skin ageing , 2011, Journal of the European Academy of Dermatology and Venereology : JEADV.

[16]  M. Shih,et al.  Protective effects of Chlorella-derived peptide on UVB-induced production of MMP-1 and degradation of procollagen genes in human skin fibroblasts. , 2011, Regulatory toxicology and pharmacology : RTP.

[17]  A. Maria,et al.  Ecdysteroids from Chenopodium quinoa Willd., an ancient Andean crop of high nutritional value , 2011 .

[18]  S. González,et al.  Beneficial Regulation of Matrix Metalloproteinases for Skin Health , 2011, Enzyme research.

[19]  J. Wessels,et al.  The effects of 20-hydroxyecdysone and 17&bgr;-estradiol on the skin of ovariectomized rats , 2011, Menopause.

[20]  C. Luo,et al.  Protective effects of 20‐hydroxyecdysone on CoCl2‐induced cell injury in PC12 cells , 2010, Journal of cellular biochemistry.

[21]  Luis Puente,et al.  Nutrition facts and functional potential of quinoa (Chenopodium quinoa willd.), an ancient Andean grain: a review. , 2010, Journal of the science of food and agriculture.

[22]  T. Slaga,et al.  Final Report of the Safety Assessment of Kojic Acid as Used in Cosmetics , 2010, International journal of toxicology.

[23]  M. Martí,et al.  Inhibitory effect of quercetin on matrix metalloproteinase 9 activity molecular mechanism and structure-activity relationship of the flavonoid-enzyme interaction. , 2010, European journal of pharmacology.

[24]  I. Raskin,et al.  Ecdysteroids elicit a rapid Ca2+ flux leading to Akt activation and increased protein synthesis in skeletal muscle cells , 2010, Steroids.

[25]  H. Jarry,et al.  Beta-ecdysone has bone protective but no estrogenic effects in ovariectomized rats. , 2010, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[26]  J. Grant-Kels,et al.  Healing fats of the skin: the structural and immunologic roles of the omega-6 and omega-3 fatty acids. , 2010, Clinics in dermatology.

[27]  Kyoung-Chan Park,et al.  Biology of melanogenesis and the search for hypopigmenting agents , 2010 .

[28]  L. Alvarez-Jubetea,et al.  Nutritive value of pseudocereals and their increasing use as functional gluten-free ingredients , 2010 .

[29]  R. Kizek,et al.  Matrix metalloproteinases. , 2010, Current medicinal chemistry.

[30]  S. Katiyar,et al.  Skin photoprotection by natural polyphenols: anti-inflammatory, antioxidant and DNA repair mechanisms , 2010, Archives of Dermatological Research.

[31]  L. Dinan.  The Karlson Lecture. Phytoecdysteroids: what use are they? , 2009, Archives of insect biochemistry and physiology.

[32]  H. Maibach,et al.  Role of topical peptides in preventing or treating aged skin , 2009, International journal of cosmetic science.

[33]  I. Raskin,et al.  A natural history of botanical therapeutics. , 2008, Metabolism: clinical and experimental.

[34]  H. Kikuzaki,et al.  Ecdysteroids act as inhibitors of calf skin collagenase and oxidative stress , 2008, Journal of biochemical and molecular toxicology.

[35]  A. Papatsoris,et al.  Matrix metalloproteinase inhibitors as anticancer agents. , 2008, The international journal of biochemistry & cell biology.

[36]  Y. Helfrich,et al.  Overview of skin aging and photoaging. , 2008, Dermatology nursing.

[37]  Jun-hua Zhang,et al.  [Preparation and dose-effect analysis of ecdysterone cream for promoting wound healing]. , 2008, Nan fang yi ke da xue xue bao = Journal of Southern Medical University.

[38]  I. Raskin,et al.  Phytoecdysteroids increase protein synthesis in skeletal muscle cells. , 2008, Journal of agricultural and food chemistry.

[39]  R. Watson,et al.  Repair of photoaged dermal matrix by topical application of a cosmetic ‘antiageing’ product , 2007, The British journal of dermatology.

[40]  Y. Helfrich,et al.  Overview of Skin Aging and Photoaging DERMATOLOGY NURSING , 2008 .

[41]  H. Lim,et al.  Inhibition of Mammalian Collagenase, Matrix Metalloproteinase-1, by Naturally-Occurring Flavonoids , 2007, Planta medica.

[42]  Z. Draelos.  The latest cosmeceutical approaches for anti‐aging , 2007 .

[43]  C. Boeriu,et al.  Novel peptides with tyrosinase inhibitory activity , 2007, Peptides.

[44]  Marcia Ramos‐e‐Silva,et al.  Elderly skin and its rejuvenation: products and procedures for the aging skin , 2007, Journal of cosmetic dermatology.

[45]  S. Gorinstein,et al.  The total polyphenols and the antioxidant potentials of some selected cereals and pseudocereals , 2007 .

[46]  J. Chung,et al.  Photoprotective and anti-skin-aging effects of eicosapentaenoic acid in human skin in vivo Published, JLR Papers in Press, February 7, 2006. , 2006, Journal of Lipid Research.

[47]  J. W. Von den Hoff,et al.  Zymographic techniques for the analysis of matrix metalloproteinases and their inhibitors. , 2005, BioTechniques.

[48]  G. Fisher,et al.  UV-light-induced signal cascades and skin aging , 2002, Ageing Research Reviews.

[49]  Pauline M. Rudd,et al.  Biochemistry and Molecular Biology of Gelatinase B or Matrix Metalloproteinase-9 (MMP-9) , 2002, Critical reviews in biochemistry and molecular biology.

[50]  K. Scharffetter-Kochanek,et al.  Solar UV irradiation and dermal photoaging. , 2001, Journal of photochemistry and photobiology. B, Biology.

[51]  E. LaVoie,et al.  ANTIOXIDATIVE FLAVONOID GLYCOSIDES FROM QUINOA SEEDS (CHENOPODIUM QUINOA WILLD) , 2001 .

[52]  R. Homan,et al.  Rapid separation and quantitation of combined neutral and polar lipid classes by high-performance liquid chromatography and evaporative light-scattering mass detection. , 1998, Journal of chromatography. B, Biomedical sciences and applications.

[53]  Robert C. Wolpert,et al.  A Review of the , 1985 .

[54]  I. K. Cohen,et al.  Biology of wound healing. , 1984, The Surgical clinics of North America.