Development and comparative study of different immunoenzyme techniques for pesticides detection

Abstract Different ELISA techniques have been developed for the detemination of four widely used pesticides: 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), simazine and atrazine. Dependences between the assay scheme and the limiting detectable concentration of the pesticide were studied. The cases of preferential applying of the scheme with immobilized antibodies or one with immobilized pesticide-protein conjugate have been revealed. The following approaches resulting in lowering of ELISA sensitivity were proposed: preliminary incubation of the tested sample with antibodies, immobilization of antibodies via staphylococcal protein A, usage of monovalent fragments of antibodies instead of native ones and chemical modification of the pesticide molecules in the sample. Optimal combinations of these approaches permitted to lower the detection limit of the assays in about 5–30 times. The achieved sensitivities were 3 ng/mL for 2,4-D, 5 ng/mL for 2,4,5-T, 0.05 ng/mL for sima...

[1]  D. Knopp,et al.  Assessment of exposure to 2,4-dichlorophenoxyacetic acid in the chemical industry: results of a five year biological monitoring study. , 1994, Occupational and environmental medicine.

[2]  B. Hock,et al.  Improved enzyme immunoassay for the analysis of s‐triazines in water samples , 1989 .

[3]  R. Fields.  The measurement of amino groups in proteins and peptides. , 1971, The Biochemical journal.

[4]  J. N. Miller.  Advances in steroid analysis : edited by S. Görög. Proceedings of the Symposium on the Analysis of Steroids, Eger, Hungary: 20–22 May 1981. Elsevier, Amsterdam, 1982, pp. xi + 552, ISBN 963-05-3009-0. , 1983 .

[5]  J. Hall,et al.  Immunoassays for the detection of 2,4-D and picloram in river water and urine , 1989 .

[6]  Eline P. Meulenberg,et al.  Water quality control in the production of drinking water from river water. The application of immunological techniques for the detection of chlorophenoxy acid herbicides (2,4-D) , 1995 .

[7]  J. Tekel,et al.  Chromatographic methods in the determination of herbicide residues in crops, food and environmental samples. , 1993, Journal of chromatography.

[8]  B. Hock,et al.  Analysis of atrazine residues in food by an enzyme immunoassay , 1993 .

[9]  H. Lenhoff,et al.  Enzyme-Mediated Immunoassay , 1985, Springer US.

[10]  Bruce D. Hammock,et al.  Use of Immunochemical Techniques for the Analysis of Pesticides , 1989 .

[11]  Vladimir Kolar,et al.  Monoclonal Elisa for 2,4-Dichlorophenoxyacetic Acid: Characterization of Antibodies and Assay Optimization , 1994 .

[12]  F. Stevens,et al.  Modification of an ELISA-based procedure for affinity determination: correction necessary for use with bivalent antibody. , 1987, Molecular immunology.

[13]  D. Knopp,et al.  Biological monitoring of 2,4-dichlorophenoxyacetic acid-exposed workers in agriculture and forestry , 1991, International archives of occupational and environmental health.

[14]  S. Yoshitake,et al.  Enzyme-labeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining. , 1983, Journal of immunoassay.

[15]  W. Knapp,et al.  Immunofluorescence and related staining techniques , 1978 .

[16]  D. Barceló,et al.  Determination of atrazine and alachlor in natural waters by a rapid-magnetic particle-based ELISA. Influence of common cross-reactants deethylatrazine, deisopropylatrazine, simazine and metolachlor , 1995 .

[17]  J. Beatty,et al.  Measurement of monoclonal antibody affinity by non-competitive enzyme immunoassay. , 1987, Journal of immunological methods.

[18]  G. Niswender,et al.  Development of enzyme immunoassay for the detection of triazine herbicides , 1990 .

[19]  S. Eremin,et al.  Enzyme immunoassays for s-triazine herbicides and their application in environmental and food analysis , 1995 .

[20]  T. Giersch.  A new monoclonal antibody for the sensitive detection of atrazine with immunoassay in microtiter plate and dipstick format , 1993 .

[21]  Andreas Brecht,et al.  A direct optical immunosensor for atrazine detection , 1995 .

[22]  D. Knopp.  Application of immunological methods for the determination of environmental pollutants in human biomonitoring. A review , 1995 .

[23]  M. Vanderlaan,et al.  ES Critical Review: Environmental monitoring by immunoassay. , 1988, Environmental science & technology.

[24]  A. Habeeb,et al.  Determination of free amino groups in proteins by trinitrobenzenesulfonic acid. , 1966, Analytical biochemistry.

[25]  Anatoly V. Zherdev,et al.  Immunodetection of Herbicide 2,4-Dichlorophenoxyacetic Acid by Field-Effect Transistor-Based Biosensors , 1994 .

[26]  J. Schlaeppi,et al.  Hydroxyatrazine and atrazine determination in soil and water by enzyme-linked immunosorbent assay using specific monoclonal antibodies , 1989 .

[27]  B. Hock.  Enzyme Immunoassay for Pesticide Analysis Enzymimmunoassays zur Pestizidanalytik , 1993 .

[28]  W. Heineman,et al.  Capillary Enzyme Immunoassay with Electrochemical Detection for the Determination of Atrazine in Water , 1995 .

[29]  Bruce D. Hammock,et al.  Hapten synthesis, antibody development, and competitive inhibition enzyme immunoassay for s-triazine herbicides. , 1990 .