Application of response surface methodology (RSM) for optimization of nutrient supplementation for Cr (VI) removal by Aspergillus lentulus AML05.

Response surface methodology (RSM) involving central composite design (CCD) was employed to optimize the concentrations of different media components for growth and Cr (VI) removal (100 mg l(-1) initial concentration) by Aspergillus lentulus AML05 at pH 6.5. The interaction between five variables i.e. glucose, K(2)HPO(4), MgSO(4), yeast extract, NH(4)NO(3) was studied and modelled. The statistical analysis of the results showed that in the range studied, yeast extract had a significant effect on Cr (VI) removal and production of fungal biomass. The optimum combination predicted via RSM was confirmed through experiment, whereby almost complete removal (99.8%) of Cr (VI) was obtained within 120 h. The validation of these results in terms of field applicability was also tested by treating industrial effluent supplemented with key media components.

[1]  J. Nielsen,et al.  Characterization of a simple bacterial consortium for effective treatment of wastewaters with reactive dyes and Cr(VI). , 2007, Chemosphere.

[2]  Abdurrahman Tanyolaç,et al.  Optimization of electrochemical treatment of industrial paint wastewater with response surface methodology. , 2007, Journal of hazardous materials.

[3]  T. E. Abraham,et al.  Studies on chromium(VI) adsorption-desorption using immobilized fungal biomass. , 2003, Bioresource technology.

[4]  P. Gunasekaran,et al.  Optimization of medium composition for alkali-stable xylanase production by Aspergillus fischeri Fxn 1 in solid-state fermentation using central composite rotary design. , 2005, Bioresource technology.

[5]  M. G. Dastidar,et al.  Zinc Uptake by Fungal Biomass Isolated from Industrial Wastewater , 2002 .

[6]  Filiz Karacan,et al.  Optimization of manufacturing conditions for activated carbon from Turkish lignite by chemical activation using response surface methodology , 2007 .

[7]  Kun-Nan Chen,et al.  Optimization on response surface models for the optimal manufacturing conditions of dairy tofu , 2005 .

[8]  H. Eccles,et al.  Treatment of metal-contaminated wastes: why select a biological process? , 1999, Trends in biotechnology.

[9]  Wen-rong Hu,et al.  Biodegradation mechanisms and kinetics of azo dye 4BS by a microbial consortium. , 2004, Chemosphere.

[10]  Yusuf Kaya,et al.  Response surface optimization of the removal of nickel from aqueous solution by cone biomass of Pinus sylvestris. , 2006, Bioresource technology.

[11]  A. Juwarkar,et al.  Biological removal of Cr (VI) by bacterial isolates obtained from metal contaminated sites , 2008, Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering.

[12]  R. H. Myers,et al.  Response Surface Methodology: Process and Product Optimization Using Designed Experiments , 1995 .

[13]  P. Ramteke,et al.  Chromium (VI) biosorption and bioaccumulation by chromate resistant bacteria. , 2002, Chemosphere.

[14]  S. Se.  Plating and cyanide wastes. , 1972, Journal - Water Pollution Control Federation.

[15]  O. Muter,et al.  Interrelations of the yeast Candida utilis and Cr(VI): metal reduction and its distribution in the cell and medium , 2001 .

[16]  M. Arısoy,et al.  Biological and chemical removal of Cr(VI) from waste water: cost and benefit analysis. , 2007, Journal of hazardous materials.

[17]  S. Das,et al.  Biosorption of chromium by Termitomyces clypeatus. , 2007, Colloids and surfaces. B, Biointerfaces.

[18]  D. Madamwar,et al.  Response surface methodology for optimization of medium for decolorization of textile dye Direct Black 22 by a novel bacterial consortium. , 2008, Bioresource technology.

[19]  A. Tripathi,et al.  Survival and chromate reducing ability of Pseudomonas aeruginosa in industrial effluents , 1999, Letters in applied microbiology.

[20]  Kannan Pakshirajan,et al.  Optimization of batch process parameters using response surface methodology for dye removal by a novel adsorbent , 2005 .

[21]  Z. Aksu,et al.  Bioaccumulation of copper(II), lead(II) and chromium(VI) by growing Aspergillus niger , 2003 .

[22]  Anushree Malik,et al.  Metal bioremediation through growing cells. , 2004, Environment international.

[23]  E. R. El-Helow,et al.  Citric acid production by a novel Aspergillus niger isolate: II. Optimization of process parameters through statistical experimental designs. , 2007, Bioresource technology.

[24]  I. Thakur,et al.  Isolation and process parameter optimization of Aspergillus sp. for removal of chromium from tannery effluent. , 2006, Bioresource technology.

[25]  Z. Aksu,et al.  Use of response surface methodology (RSM) in the evaluation of growth and copper(II) bioaccumulation properties of Candida utilis in molasses medium. , 2008, Journal of hazardous materials.

[26]  Jo‐Shu Chang,et al.  Decolorization of azo dyes with immobilized Pseudomonas luteola , 2001 .

[27]  C. Kaushik,et al.  Response surface methodological approach for optimizing removal of Cr (VI) from aqueous solution using immobilized cyanobacterium , 2007 .