Optimization of Conditions for Cyanidin-3-O-Glucoside (C3G) Nanoliposome Production by Response Surface Methodology and Cellular Uptake Studies in Caco-2 Cells

We aimed to optimize the formulation of C3G nanoliposomes using response surface methodology. Additionally, we evaluated the stability, particle change, and encapsulation efficiency (EE) of C3G nanoliposomes under different temperatures and storage durations, as well as in simulated gastrointestinal juice (SGF) and simulated intestinal fluid. The morphology of C3G nanoliposomes was observed by transmission electron microscope. The ability of C3G nanoliposomes to affect cancer cell morphology and inhibit cancer cell proliferation was studied with Caco-2 cells. Reverse-phase evaporation method is a simple and efficient method for liposome preparation. The optimal preparation conditions for this method were as follows: C3G concentration of 0.17 mg/mL, phosphatidylcholine/cholesterol ratio of 2.87, and rotary evaporation temperature of 41.41 °C. At optimal conditions, the particle size and EE of the C3G nanoliposomes were 165.78 ± 4.3 nm and 70.43% ± 1.95%, respectively. The C3G nanoliposomes showed an acceptable stability in SGF at 37 °C for 4 h, but were unstable under extended storage durations and high temperatures. Moreover, our results showed that different concentrations of C3G nanoliposomes affected the morphology and inhibited the proliferation of Caco-2 cells.

[1]  Yang Yang,et al.  Preparation and Evaluation of Oxaliplatin Thermosensitive Liposomes with Rapid Release and High Stability , 2016, PloS one.

[2]  F. Dorkoosh,et al.  Liposomal formulation for co-delivery of paclitaxel and lapatinib, preparation, characterization and optimization , 2016, Journal of liposome research.

[3]  C. Nastruzzi,et al.  "Off-the-shelf" microfluidic devices for the production of liposomes for drug delivery. , 2016, Materials science & engineering. C, Materials for biological applications.

[4]  Dawei Gao,et al.  Antitumor drug effect of betulinic acid mediated by polyethylene glycol modified liposomes. , 2016, Materials science & engineering. C, Materials for biological applications.

[5]  H. Xiong,et al.  Juglone Thermosensitive Liposomes: Preparation, Characterization, in vitro Release and Hyperthermia Cell Evaluation , 2016 .

[6]  Xianjun Meng,et al.  Cyanidin-3-O-glucoside Induces Apoptosis and Inhibits Migration of Tumor Necrosis Factor-α-Treated Rat Aortic Smooth Muscle Cells , 2016, Cardiovascular Toxicology.

[7]  Rongfa Guan,et al.  Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability , 2016, Molecules.

[8]  H. Takeuchi,et al.  Pulmonary liposomal formulations encapsulated procaterol hydrochloride by a remote loading method achieve sustained release and extended pharmacological effects. , 2016, International journal of pharmaceutics.

[9]  Wei Huang,et al.  Optimization of the process variables of tilianin-loaded composite phospholipid liposomes based on response surface-central composite design and pharmacokinetic study. , 2016, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[10]  D. Singh,et al.  Preparation and optimization of quercetin-loaded liposomes for wound healing, using response surface methodology , 2016, Artificial cells, nanomedicine, and biotechnology.

[11]  S. Kallithraka,et al.  Effect of irrigation regime on anthocyanin content and antioxidant activity of Vitis vinifera L. cv. Syrah grapes under semiarid conditions. , 2016, Journal of the science of food and agriculture.

[12]  V. de Freitas,et al.  Antioxidant and antiproliferative properties of 3-deoxyanthocyanidins. , 2016, Food chemistry.

[13]  Lugang Zhang,et al.  Anthocyanin Accumulation, Antioxidant Ability and Stability, and a Transcriptional Analysis of Anthocyanin Biosynthesis in Purple Heading Chinese Cabbage (Brassica rapa L. ssp. pekinensis). , 2016, Journal of agricultural and food chemistry.

[14]  Huan Xu,et al.  Influence of type and proportion of lyoprotectants on lyophilized ginsenoside Rg3 liposomes , 2016, The Journal of pharmacy and pharmacology.

[15]  L. Szente,et al.  Evaluation of the Cytotoxicity of α-Cyclodextrin Derivatives on the Caco-2 Cell Line and Human Erythrocytes , 2015, Molecules.

[16]  D. Muntean,et al.  Optimizing long-circulating liposomes for delivery of simvastatin to C26 colon carcinoma cells , 2015, Journal of liposome research.

[17]  Xudong Yao,et al.  Oral Delivery of Bovine Lactoferrin Using Pectin‐ and Chitosan‐Modified Liposomes and Solid Lipid Particles: Improvement of Stability of Lactoferrin , 2015, Chemical biology & drug design.

[18]  N. Saari,et al.  Preparation and characterisation of nanoliposomes containing winged bean seeds bioactive peptides , 2015, Journal of microencapsulation.

[19]  L. Kiew,et al.  A Comparative Approach for the Preparation and Physicochemical Characterization of Lecithin Liposomes Using Chloroform and Non-Halogenated Solvents , 2015 .

[20]  F. Temelli,et al.  Preparation of liposomes using a modified supercritical process via depressurization of liquid phase , 2015 .

[21]  Young Eon Kim,et al.  Cyanidin-3-glucoside isolated from mulberry fruits protects pancreatic β-cells against glucotoxicity-induced apoptosis. , 2015, Molecular medicine reports.

[22]  D. Lamprou,et al.  Influence of cholesterol on liposome stability and on in vitro drug release , 2015, Drug Delivery and Translational Research.

[23]  H. Byrne,et al.  Cell viability assessment using the Alamar blue assay: a comparison of 2D and 3D cell culture models. , 2015, Toxicology in vitro : an international journal published in association with BIBRA.

[24]  K. Hwang,et al.  Antioxidant and antiinflammatory activities of cyanidin-3-glucoside and cyanidin-3-rutinoside in hydrogen peroxide and lipopolysaccharide-treated RAW264.7 cells , 2014, Food Science and Biotechnology.

[25]  R. Reis,et al.  Liposomes in tissue engineering and regenerative medicine , 2014, Journal of The Royal Society Interface.

[26]  Xianglin Shi,et al.  Cyanidin-3-glucoside inhibits UVB-induced oxidative damage and inflammation by regulating MAP kinase and NF-κB signaling pathways in SKH-1 hairless mice skin. , 2014, Toxicology and applied pharmacology.

[27]  Tang-Bin Zou,et al.  The Role of Sodium-Dependent Glucose Transporter 1 and Glucose Transporter 2 in the Absorption of Cyanidin-3-O-β-Glucoside in Caco-2 Cells , 2014, Nutrients.

[28]  Xiaobo Luo,et al.  Optimization on condition of epigallocatechin-3-gallate (EGCG) nanoliposomes by response surface methodology and cellular uptake studies in Caco-2 cells , 2014, Nanoscale Research Letters.

[29]  S. Abbas,et al.  Liposomes as delivery systems for carotenoids: comparative studies of loading ability, storage stability and in vitro release. , 2014, Food & function.

[30]  Yongheng Bai,et al.  Effect of Sedum sarmentosum BUNGE extract on aristolochic acid-induced renal tubular epithelial cell injury. , 2014, Journal of pharmacological sciences.

[31]  Xingqian Ye,et al.  Response surface methodology for the optimization of beta-lactoglobulin nano-liposomes. , 2014, Food & function.

[32]  Yuanliang Hu,et al.  The preparation of gypenosides liposomes and its effects on the peritoneal macrophages function in vitro. , 2014, International journal of pharmaceutics.

[33]  Yingjian Lu,et al.  Effects of baking on cyanidin-3-glucoside content and antioxidant properties of black and yellow soybean crackers. , 2013, Food chemistry.

[34]  Haibao Peng,et al.  Preparation of hierarchical mesoporous CaCO3 by a facile binary solvent approach as anticancer drug carrier for etoposide , 2013, Nanoscale Research Letters.

[35]  Zhi-Qing Yuan,et al.  Dexamethasone-induced apoptosis in PC12 cells , 2013 .

[36]  Yihang Wu,et al.  Development and characterization of lactoferrin nanoliposome: cellular uptake and stability , 2012, Nanoscale Research Letters.

[37]  Rongfa Guan,et al.  Cytotoxicity, oxidative stress, and genotoxicity in human hepatocyte and embryonic kidney cells exposed to ZnO nanoparticles , 2012, Nanoscale Research Letters.

[38]  Qinmei Zhou,et al.  Preparation and characterization of gemcitabine liposome injections. , 2012, Pharmazie.

[39]  C. Baravian,et al.  Elaboration and characterization of nanoliposome made of soya; rapeseed and salmon lecithins: application to cell culture. , 2012, Colloids and surfaces. B, Biointerfaces.

[40]  Jian-Guo Jiang,et al.  Preparation of a tea polyphenol nanoliposome system and its physicochemical properties. , 2011, Journal of agricultural and food chemistry.

[41]  Yanli Chang,et al.  In vitro toxicity evaluation of graphene oxide on A549 cells. , 2011, Toxicology letters.

[42]  O. Paredes-López,et al.  Inhibition of pro-inflammatory responses and antioxidant capacity of Mexican blackberry (Rubus spp.) extracts. , 2010, Journal of agricultural and food chemistry.

[43]  Yi Huang,et al.  Upregulation of the GRIM-19 gene suppresses invasion and metastasis of human gastric cancer SGC-7901 cell line. , 2010, Experimental cell research.

[44]  Hua Xiong,et al.  Vanillin cross-linked chitosan microspheres for controlled release of resveratrol , 2010 .

[45]  M. Mi,et al.  Structural requirements of anthocyanins in relation to inhibition of endothelial injury induced by oxidized low‐density lipoprotein and correlation with radical scavenging activity , 2010, FEBS letters.

[46]  Jin Chang,et al.  Characterization of novel multifunctional cationic polymeric liposomes formed from octadecyl quaternized carboxymethyl chitosan/cholesterol and drug encapsulation. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[47]  Shiying Xu,et al.  Preparation of salidroside nano-liposomes by ethanol injection method and in vitro release study , 2008 .

[48]  Xiaoming Zhang,et al.  Effect of different preparation methods on physicochemical properties of salidroside liposomes. , 2007, Journal of agricultural and food chemistry.

[49]  E. Disalvo,et al.  Characterization of liposomes coated with S-layer proteins from lactobacilli. , 2007, Biochimica et biophysica acta.

[50]  S. Chu,et al.  Cyanidin 3-Glucoside and Peonidin 3-Glucoside Inhibit Tumor Cell Growth and Induce Apoptosis In Vitro and Suppress Tumor Growth In Vivo , 2005, Nutrition and cancer.

[51]  G. Yen,et al.  Effects of anthocyanidin on the inhibition of proliferation and induction of apoptosis in human gastric adenocarcinoma cells. , 2005, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[52]  E. Kowalczyk,et al.  [The use of anthocyanins in the treatment of cardiovascular diseases]. , 2005, Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego.

[53]  Theresa M Allen,et al.  Drug release rate influences the pharmacokinetics, biodistribution, therapeutic activity, and toxicity of pegylated liposomal doxorubicin formulations in murine breast cancer. , 2004, Biochimica et biophysica acta.

[54]  P. Walde,et al.  The mechanism of liposomal damage by taurocholate. , 1987, Biochimica et biophysica acta.

[55]  Yikun Gao,et al.  Facile synthesis of the lipid bilayer coated mesoporous silica nanocomposites and their application in drug delivery , 2016 .

[56]  J. Aravind,et al.  Optimization of chromium(VI) biosorption using gooseberry seeds by response surface methodology , 2016 .

[57]  E. Reverchon,et al.  Efficient encapsulation of proteins in submicro liposomes using a supercritical fluid assisted continuous process , 2016 .

[58]  L. Kiew,et al.  Optimization of Phospholipid Nanoparticle Formulations Using Response Surface Methodology , 2016 .

[59]  N. P. Ulrih,et al.  Liposomal stabilization of ascorbic acid in model systems and in food matrices , 2012 .