The Nutrition of Brucellae: Growth in Simple Chemically Defined Media

The nutritional requirements of brucellae grown in chemically defined media have been investigated by ZoBell and Meyer (1932a,b), Koser, Breslove, and Dorfman (1941), N. B. McCullough and Dick (1942a,b, 1943), W. G. McCullough et al. (1947), and others. With one exception (McCullough and Dick, 1943), however, the media employed seemed unduly complex by reason of the number of amino acids required in their preparation. Utilization of inorganic ammonium salts as the sole nitrogen source for these organisms has been demonstrated by ZoBell and Meyer (1932a), McCullough and Dick (1943), and Gerhardt and Wilson (1947). Consequently, the present study was undertaken in an effort to obtain a simple, chemically defined medium for cultivation of brucellae. Interest in this laboratory has centered on the "avirulent" Brucela abortue strain 19, widely used for animal immunization. The nutritional requirements of this culture were studied intensively and the findings applied subsequently to a number of other strains.

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