β-Lactamases of Kluyvera ascorbata, Probable Progenitors of Some Plasmid-Encoded CTX-M Types

ABSTRACT Kluyvera ascorbata produces a β-lactamase that results in an atypical susceptibility pattern, including low-level resistance to penicillins, cephalothin, and cefuroxime, but this resistance is reversed by clavulanate. Ten nucleotide sequences of the corresponding gene, blaKLUA, were obtained and were found to have minor variations (96 to 100%). Otherwise, blaKLUA was found to be similar (95 to 100%) to some plasmid-encoded CTX-M-type β-lactamases. Finally, mobilization of blaKLUA on a plasmid was found to be mediated probably by a genetic mobile element like ISEcp1.

[1]  George A. Jacoby,et al.  Plasmid-Determined AmpC-Type β-Lactamases , 2002, Antimicrobial Agents and Chemotherapy.

[2]  P. Nordmann,et al.  Characterization of a Chromosomally Encoded Extended-Spectrum Class A β-Lactamase from Kluyvera cryocrescens , 2001, Antimicrobial Agents and Chemotherapy.

[3]  P. Bradford.  Extended-Spectrum β-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat , 2001, Clinical Microbiology Reviews.

[4]  V. Jarlier,et al.  Novel Class A β-Lactamase Sed-1 fromCitrobacter sedlakii: Genetic Diversity of β-Lactamases within the Citrobacter Genus , 2001, Antimicrobial Agents and Chemotherapy.

[5]  P. Nordmann,et al.  Plasmid-mediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1. , 2001, FEMS microbiology letters.

[6]  C. Hart,et al.  Molecular Characterization of a Novel Plasmid-Encoded Cefotaximase (CTX-M-12) Found in Clinical Klebsiella pneumoniae Isolates from Kenya , 2001, Antimicrobial Agents and Chemotherapy.

[7]  A. Oliver,et al.  Nucleotide Sequence and Characterization of a Novel Cefotaxime-Hydrolyzing β-Lactamase (CTX-M-10) Isolated in Spain , 2001, Antimicrobial Agents and Chemotherapy.

[8]  J. Barbé,et al.  Cloning and Sequence of the Gene Encoding a Novel Cefotaxime-Hydrolyzing β-Lactamase (CTX-M-9) fromEscherichia coli in Spain , 2000, Antimicrobial Agents and Chemotherapy.

[9]  R. Bonnet,et al.  A Novel CTX-M β-Lactamase (CTX-M-8) in Cefotaxime-ResistantEnterobacteriaceae Isolated in Brazil , 2000, Antimicrobial Agents and Chemotherapy.

[10]  J. Herrmann,et al.  Outbreak of Klebsiella pneumoniae producing transferable AmpC-type beta-lactamase (ACC-1) originating from Hafnia alvei. , 2000, FEMS microbiology letters.

[11]  L. Tzouvelekis,et al.  CTX-M-type β-lactamases: an emerging group of extended-spectrum enzymes , 2000 .

[12]  J. Barbé,et al.  Cloning and Sequence of the Gene Encoding a Novel Cefotaxime-Hydrolyzing b-Lactamase (CTX-M-9) from Escherichia coli in Spain , 2000 .

[13]  M. Inoue,et al.  Characterization of SFO-1, a Plasmid-Mediated Inducible Class A β-Lactamase from Enterobacter cloacae , 1999, Antimicrobial Agents and Chemotherapy.

[14]  M. Gazouli,et al.  Two novel plasmid-mediated cefotaxime-hydrolyzing beta-lactamases (CTX-M-5 and CTX-M-6) from Salmonella typhimurium. , 1998, FEMS microbiology letters.

[15]  G. Storch,et al.  CTX-M-5, a Novel Cefotaxime-Hydrolyzing β-Lactamase from an Outbreak of Salmonella typhimuriumin Latvia , 1998, Antimicrobial Agents and Chemotherapy.

[16]  M. Ishiguro,et al.  Cloning and Sequencing of the Gene Encoding Toho-2, a Class A β-Lactamase Preferentially Inhibited by Tazobactam , 1998, Antimicrobial Agents and Chemotherapy.

[17]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[18]  P. H. Roy,et al.  Chromosomal beta-lactamase genes of Klebsiella oxytoca are divided into two main groups, blaOXY-1 and blaOXY-2 , 1996, Antimicrobial agents and chemotherapy.

[19]  石井 良和.  Cloning and sequence of the gene encoding a cefotaxime-hydrolyzing class A β-lactmase isolated from Escherichia coli , 1996 .

[20]  M. Ishiguro,et al.  Cloning and sequence of the gene encoding a cefotaxime-hydrolyzing class A beta-lactamase isolated from Escherichia coli , 1995, Antimicrobial agents and chemotherapy.

[21]  P. Grimont,et al.  Escherichia hermannii: susceptibility pattern to β-lactams and production of β-lactamase , 1995 .

[22]  H. H. Martin,et al.  A common system controls the induction of very different genes. The class-A beta-lactamase of Proteus vulgaris and the enterobacterial class-C beta-lactamase. , 1994, European journal of biochemistry.

[23]  R. Yogev,et al.  Peritonitis due to Kluyvera ascorbata: case report and review. , 1990, Reviews of infectious diseases.

[24]  D. J. Brenner,et al.  Kluyvera, a new (redefined) genus in the family Enterobacteriaceae: identification of Kluyvera ascorbata sp. nov. and Kluyvera cryocrescens sp. nov. in clinical specimens , 1981, Journal of clinical microbiology.

[25]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.