Polymerase chain reaction products analyzed by charge detection mass spectrometry

Charge detection mass spectrometry (CDMS) has been used to rapidly analyze polymerase chain reaction (PCR) products with a minimum of sample cleanup after the PCR reaction. PCR products of 1525 base pairs (bp), 1982 bp and 2677 bp were detected by CDMS, where simultaneous measurement of the charge and velocity of an electrostatically accelerated ion allows a mass determination of the ion. We extended the investigation to a longer double-stranded, linear DNA sample by also analyzing linearized pBR322 (4361 bp). Positive ion mass spectra and ion charge-state distributions were obtained for these electrosprayed DNA samples. Instrumental conditions were studied using a commercially available, linearized pUC18 sample (2682 bp). Copyright © 1999 John Wiley & Sons, Ltd.

[1]  J. C. Schultz,et al.  Mass determination of megadalton-DNA electrospray ions using charge detection mass spectrometry , 1998, Journal of the American Society for Mass Spectrometry.

[2]  A. Fox,et al.  Heterogeneity in Bacillus cereus PCR products detected by ESI-FTICR mass spectrometry. , 1998, Analytical chemistry.

[3]  Y. Wada.  Separate analysis of complementary strands of restriction enzyme-digested DNA. An application of restriction fragment mass mapping by matrix-assisted laser desorption/ionization mass spectrometry. , 1998, Journal of mass spectrometry : JMS.

[4]  W. Benner.  A Gated Electrostatic Ion Trap To Repetitiously Measure the Charge and m/z of Large Electrospray Ions , 1997 .

[5]  Y. Wada,et al.  Detection of single-nucleotide mutations including substitutions and deletions by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. , 1997, Rapid Communications in Mass Spectrometry.

[6]  R. Arakawa,et al.  Genetic diagnosis by polymerase chain reaction and electrospray ionization mass spectrometry: Detection of five base deletion from blood DNA of a familial adenomatous polyposis patient , 1997 .

[7]  A. Killeen,et al.  Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a rapid screening method to detect mutations causing Tay-Sachs disease. , 1997, Rapid Communications in Mass Spectrometry.

[8]  D. Prockop,et al.  Mass spectrometric gene diagnosis of one-base substitution from polymerase chain reaction amplified human DNA. , 1997, Rapid communications in mass spectrometry : RCM.

[9]  G A Anderson,et al.  Characterization of PCR products from bacilli using electrospray ionization FTICR mass spectrometry. , 1996, Analytical chemistry.

[10]  A. Lüchow,et al.  Detection of hepatitis B virus DNA in serum samples via nested PCR and MALDI-TOF mass spectrometry. , 1996, Genetic analysis : biomolecular engineering.

[11]  A. Fox,et al.  Analysis of double-stranded polymerase chain reaction products from the Bacillus cereus group by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. , 1996, Rapid communications in mass spectrometry : RCM.

[12]  R. Arakawa,et al.  Molecular mass measurement of polymerase chain reaction products amplified from human blood DNA by electrospray ionization mass spectrometry. , 1995, Rapid communications in mass spectrometry : RCM.

[13]  W. H. Benner,et al.  Molecular weight determination of megadalton DNA electrospray ions using charge detection time-of-flight mass spectrometry. , 1995, Rapid communications in mass spectrometry : RCM.

[14]  B. Chait,et al.  Heat-induced conformational changes in proteins studied by electrospray ionization mass spectrometry. , 1993, Analytical chemistry.

[15]  C. G. Edmonds,et al.  Solvent-induced conformational changes of polypeptides probed by electrospray-ionization mass spectrometry. , 1991, Rapid communications in mass spectrometry : RCM.

[16]  J. Clark,et al.  Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. , 1988, Nucleic acids research.