Bioaugmented hydrogen production from microcrystalline cellulose using co-culture—Clostridium acetobutylicum X9 and Ethanoigenens harbinense B49

Abstract Dark fermentation of microcrystalline cellulose to produce biohydrogen using mono-culture or co-culture of isolated strains was studied. A strain ( X 9 ) with high hydrogen yield from microcrystalline cellulose was isolated and identified to be closely affiliated with Clostridium acetobutylicum, ATCC 824. At 37 ∘ C and pH 5.0, the mono-culture of X 9 yields hydrogen with a 5-h time lag and end liquid products primarily of acetate and butyrate. The co-culture of X 9 with another strain, Ethanoigenens harbinense B 49 , which can produce hydrogen efficiently from monosaccharides but directly from microcrystalline cellulose, produced more efficiently the biohydrogen via ethanol-type fermentation metabolism compared with mono-culture X 9 test. Bioaugmentation with X 9 + B 49 improved cellulose hydrolysis and subsequent hydrogen production rates as compared with that of mono-culture bioaugmentation with X 9 .

[1]  Chiu-Yue Lin,et al.  Hydrogen production during the anaerobic acidogenic conversion of glucose , 1999 .

[2]  J C Huang,et al.  Ethanol-type fermentation from carbohydrate in high rate acidogenic reactor. , 1997, Biotechnology and bioengineering.

[3]  B. Chang,et al.  Producing hydrogen from wastewater sludge by Clostridium bifermentans. , 2003, Journal of biotechnology.

[4]  F. Kargı,et al.  Bio-hydrogen production from waste materials , 2006 .

[5]  Tong Zhang,et al.  Thermophilic H2 production from a cellulose-containing wastewater , 2003, Biotechnology Letters.

[6]  Jo‐Shu Chang,et al.  Biohydrogen Production Using Suspended and Immobilized Mixed Microflora , 2006 .

[7]  Jyh-Shyong Chang,et al.  Application of Artificial Neural Networks Coupled with Sequential Pseudo-Uniform Design to Optimization of Membrane Reactors for Hydrogen Production , 2006 .

[8]  Li,et al.  Hydrogen Production with High Evolution Rate and High Yield by Immobilized Cells of Hydrogen-producing Bacteria Strain B49 in a Column Reactor , 2002 .

[9]  J. Lay,et al.  Modeling and optimization of anaerobic digested sludge converting starch to hydrogen , 2000, Biotechnology and bioengineering.

[10]  P. Claassen,et al.  Pretreatment of Miscanthus for hydrogen production by Thermotoga elfii , 2002 .

[11]  J. Lay,et al.  Biohydrogen generation by mesophilic anaerobic fermentation of microcrystalline cellulose. , 2001, Biotechnology and bioengineering.

[12]  Baikun Li,et al.  Biohydrogen production from molasses by anaerobic fermentation with a pilot-scale bioreactor system , 2006 .

[13]  K. Hara,et al.  Continuous hydrogen production by Clostridium sp. strain no. 2 from cellulose hydrolysate in an aqueous two-phase system☆ , 1996 .

[14]  N. Mizukami,et al.  Direct conversion of cellulosic materials to hydrogen by Clostridium sp. strain no. 2 , 1995 .