Cell separations by flow cytometry.

Flow cytometry has become a standard method for separating individual subsets of cells from a heterogeneous population. Multilaser, multicolour cell sorters are increasingly common and have become more complex in recent years increasing the number of applications available. However, a cell sorting experiment is only as good as the input sample, and the preparation of this is extremely important. This chapter describes the methods used to prepare samples for flow cytometry and how they can be adapted and optimised according to cell type.

[1]  Andreas Radbruch,et al.  High gradient magnetic cell separation with MACS. , 1990, Cytometry.

[2]  Z. Darżynkiewicz,et al.  Features of apoptotic cells measured by flow cytometry. , 1992, Cytometry.

[3]  M. Melamed,et al.  Relationship between RNA content and progression of lymphocytes through S phase of cell cycle. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[4]  M. Ratinaud,et al.  Flow cytometry's contribution to the measurement of cell functions , 1993, Biology of the cell.

[5]  L A Herzenberg,et al.  Flow cytometric analysis and FACS sorting of cells based on GFP accumulation , 1999 .

[6]  Nathan C Shaner,et al.  A guide to choosing fluorescent proteins , 2005, Nature Methods.

[7]  J. Steinkamp,et al.  A new method for rapid and sensitive detection of bromodeoxyuridine in DNA-replicating cells. , 1987, Experimental cell research.

[8]  A Krishan,et al.  Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining , 1975, The Journal of cell biology.

[9]  A. Órfão,et al.  General concepts about cell sorting techniques. , 1996, Clinical biochemistry.

[10]  J. Sprent,et al.  Turnover of Naive-and Memory-phenotype T Cells , 1994 .

[11]  A. Badley,et al.  Flow cytometric measurement of telomere length. , 2000, Cytometry.

[12]  Leonore A Herzenberg,et al.  Interpreting flow cytometry data: a guide for the perplexed , 2006, Nature Immunology.

[13]  D. Berglund,et al.  Isolation of viable tumor cells following introduction of labelled antibody to an intracellular oncogene product using electroporation. , 1989, Journal of immunological methods.

[14]  R. Williamson,et al.  Segregation of ΔF508 and normal CFTR alleles in human sperm , 1993 .

[15]  J. Bauman,et al.  Fluorescent in‐situ hybridization to detect cellular RNA by flow cytometry and confocal microscopy † , 1990, Journal of microscopy.

[16]  P J Quesenberry,et al.  Fluorescence activated cell sorting: a window on the stem cell. , 2007, Current pharmaceutical biotechnology.

[17]  M. Fox,et al.  Measurement of intracellular pH using flow cytometry with carboxy-SNARF-1. , 1993, Cytometry.

[18]  James F. Leary,et al.  Chapter 20 Strategies for Rare Cell Detection and Isolation , 1994 .

[19]  L. Wheeless,et al.  Quantitative single cell analysis and sorting. , 1977, Science.

[20]  J. Stegemann,et al.  A new fluid switching flow sorter , 2004, Histochemistry.

[21]  J. Dunne,et al.  Detection of mRNA in flow-sorted cells. , 1989, Cytometry.

[22]  G P Nolan,et al.  Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[23]  L. Kamentsky,et al.  Estimation of membrane potentials of individual lymphocytes by flow cytometry. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[24]  A. Griffioen,et al.  Improved method for measuring intracellular Ca++ with fluo-3. , 1990, Cytometry.

[25]  H. Shapiro.  Practical Flow Cytometry: Shapiro/Flow Cytometry 4e , 2005 .

[26]  S. Léonce,et al.  Flow cytometry: A useful technique in the study of multidrug resistance , 1993, Biology of the cell.

[27]  X. M. Sun,et al.  Quantification of apoptosis and necrosis by flow cytometry. , 1993, Acta oncologica.