Visualization of Proteus mirabilis within the Matrix of Urease-Induced Bladder Stones during Experimental Urinary Tract Infection

ABSTRACT The virulence of a urease-negative mutant of uropathogenic Proteus mirabilis and its wild-type parent strain was assessed by using a CBA mouse model of catheterized urinary tract infection. Overall, catheterized mice were significantly more susceptible than uncatheterized mice to infection by wild-type P. mirabilis. At a high inoculum, the urease-negative mutant successfully colonized bladders of catheterized mice but did not cause urolithiasis and was still severely attenuated in its ability to ascend to kidneys. Using confocal laser scanning microscopy and scanning electron microscopy, we demonstrated the presence of P. mirabilis within the urease-induced stone matrix. Alizarin red S staining was used to detect calcium-containing deposits in bladder and kidney tissues of P. mirabilis-infected mice.

[1]  H. Mobley,et al.  In Vivo Dynamics of Type 1 Fimbria Regulation in UropathogenicEscherichia coli during Experimental Urinary Tract Infection , 2001, Infection and Immunity.

[2]  H. Franz,et al.  Lokalisation eines Harnwegsinfektes durch Nachweis von antikörperbesetzten Bakterien im Urin , 1975 .

[3]  D. Musher,et al.  Urease. The primary cause of infection-induced urinary stones. , 1976, Investigative urology.

[4]  N. Carson,et al.  Site of infection in acute urinary-tract infection in general practice. , 1971, Lancet.

[5]  S Falkow,et al.  FACS-optimized mutants of the green fluorescent protein (GFP). , 1996, Gene.

[6]  R. Russell,et al.  Contribution of Proteus mirabilis urease to persistence, urolithiasis, and acute pyelonephritis in a mouse model of ascending urinary tract infection , 1993, Infection and immunity.

[7]  R. Hausinger,et al.  Copyright � 1995, American Society for Microbiology Molecular Biology of Microbial Ureases , 1995 .

[8]  H. Schumacher,et al.  Alizarin red S staining as a screening test to detect calcium compounds in synovial fluid. , 1983, Arthritis and rheumatism.

[9]  J W Warren,et al.  Catheter-associated urinary tract infections. , 2012, Critical care nurse.

[10]  H. Mobley,et al.  Construction of a urease-negative mutant of Proteus mirabilis: analysis of virulence in a mouse model of ascending urinary tract infection , 1990, Infection and immunity.

[11]  R. Sidwell,et al.  Handbook of Animal Models of Infection , 1999 .

[12]  H. Puchtler,et al.  ON THE HISTORY AND MECHANISM OF ALIZARIN AND ALIZARIN RED S STAINS FOR CALCIUM , 1969, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[13]  W. Stamm.  Catheter-associated urinary tract infections: epidemiology, pathogenesis, and prevention. , 1991, The American journal of medicine.

[14]  J. Rassweiler,et al.  Treatment of Renal Stones by Extracorporeal Shockwave Lithotripsy , 2001, European Urology.

[15]  A. Proia,et al.  Identification of calcium oxalate crystals using alizarin red S stain. , 1985, Archives of pathology & laboratory medicine.

[16]  R. Hausinger,et al.  Microbial ureases: significance, regulation, and molecular characterization. , 1989, Microbiological reviews.

[17]  H. Takeuchi,et al.  Scanning electron microscopy detects bacteria within infection stones. , 1984, The Journal of urology.

[18]  M. Hall-Craggs,et al.  Mouse models of short- and long-term foreign body in the urinary bladder: analogies to the bladder segment of urinary catheters. , 1991, Laboratory animal science.

[19]  H. Mobley,et al.  Urease-positive bacteriuria and obstruction of long-term urinary catheters , 1987, Journal of clinical microbiology.

[20]  H. Mobley,et al.  Pathogenesis of Proteus mirabilis urinary tract infection. , 2000, Microbes and infection.

[21]  W. Anthony,et al.  A prospective microbiologic study of bacteriuria in patients with chronic indwelling urethral catheters. , 1982, The Journal of infectious diseases.

[22]  K. Holmgren.  Urinary calculi and urinary tract infection. A clinical and microbiological study. , 1986, Scandinavian journal of urology and nephrology. Supplementum.

[23]  H. Mobley,et al.  Proteus mirabilis urease: nucleotide sequence determination and comparison with jack bean urease , 1989, Journal of bacteriology.

[24]  D. Johnson,et al.  Mouse Model of Ascending UTI Involving Short- and Long-term Indwelling Catheters , 1999 .

[25]  D. Griffith.  Urease stones , 2004, Urological Research.

[26]  T. D. Cohen,et al.  Struvite calculi. , 1996, Seminars in Nephrology.

[27]  S. Whitson,et al.  A simple method using alizarin red S for the detection of calcium in epoxy resin embedded tissue. , 1986, Stain technology.

[28]  T. Yasue.  HISTOCHEMICAL IDENTIFICATION OF CALCIUM OXALATE , 1969 .