Changes in the relative abundance of mRNA transcripts for insulin-like growth factor (IGF-I and IGF-II) ligands and their receptors (IGF-IR/IGF-IIR) in preimplantation bovine embryos derived from different in vitro systems.

The aim of this study was to determine the relative abundance of mRNAs for the insulin-like growth factor I (IGF-I) and IGF-II ligands, and for the IGF receptors (IGF-IR and IGF-IIR) in in vitro preimplantation bovine embryos from the oocyte to the hatched blastocyst stage using two different culture systems: TCM-199 supplemented with oestrous cow serum, or synthetic oviduct fluid supplemented with polyvinyl alcohol. Development to the two- to four-cell stage and blastocyst stage was significantly higher (P < or = 0.05) in embryos cultured in TCM supplemented with oestrous cow serum than in those cultured in synthetic oviduct fluid supplemented with polyvinyl alcohol (61 and 25% versus 55 and 17%, respectively). A semi-quantitative RT-PCR assay did not detect IGF-I transcripts at any stage of preimplantation bovine development, including the hatched blastocyst stage. In both culture systems, IGF-IR, IGF-II and IGF-IIR were expressed throughout preimplantation development up to the hatched blastocyst stage in a varying pattern. The expression patterns of IGF-IR, IGF-II and IGF-IIR in embryos generated in the two culture systems were not significantly different, except at the expanded blastocyst stage, at which significantly higher amounts of IGF-IIR were observed in the TCM system than in the synthetic oviduct fluid system. These results indicate that transcripts of IGF-IR and IGF-IIR follow the standard pattern in which maternal stores of mRNA in the oocyte are slowly depleted up to the 16-cell stage and then re-established at the onset of embryonic expression of these genes. The lack of detectable IGF-I transcripts in the bovine embryo indicates a predominantly paracrine mode of action. The bovine embryo is capable of producing IGF-II, IGF-IIR and IGF-IR in large amounts, particularly after hatching, which may be important for the formation of the filamentous conceptus. Results indicate an autocrine mechanism for IGF-II and modulation of IGF family expression by culture conditions.

[1]  A. Kouba,et al.  Oviductal regulation of fertilization and early embryonic development. , 2020, Journal of reproduction and fertility. Supplement.

[2]  B. Brackett,et al.  Effects of culture system and protein supplementation on mRNA expression in pre-implantation bovine embryos. , 2001, Human reproduction.

[3]  D. Armstrong,et al.  Effect of Cell-to-Cell Contact on In Vitro Deoxyribonucleic Acid Synthesis and Apoptosis Responses of Bovine Granulosa Cells to Insulin-Like Growth Factor-I and Epidermal Growth Factor1 , 2000, Biology of reproduction.

[4]  A. Gutiérrez-Adán,et al.  Relationship between time of first cleavage and the expression of IGF‐I growth factor, its receptor, and two housekeeping genes in bovine two‐cell embryos and blastocysts produced in vitro , 2000, Molecular reproduction and development.

[5]  A. Crosier,et al.  In Vitro Production of Embryos Alters Levels of Insulin-like Growth Factor-II Messenger Ribonucleic Acid in Bovine Fetuses 63 Days After Transfer1 , 2000, Biology of reproduction.

[6]  S. Garg,et al.  Expression of growth factor ligand and receptor genes in preimplantation stage water buffalo (Bubalus bubalis) embryos and oviduct epithelial cells. , 1999, Journal of reproduction and fertility.

[7]  C. Wrenzycki,et al.  Alterations in the relative abundance of gene transcripts in preimplantation bovine embryos cultured in medium supplemented with either serum or PVA , 1999, Molecular reproduction and development.

[8]  H. M. Beier,et al.  Insulin and insulin-like growth factor-I promote rabbit blastocyst development and prevent apoptosis. , 1998, Biology of reproduction.

[9]  S. Hamano,et al.  Expression of growth factor ligand and their receptor mRNAs in bovine ova during in vitro maturation and after fertilization in vitro. , 1998, The Journal of veterinary medical science.

[10]  S. Totey,et al.  Effect of insulin‐like growth factor I and its interaction with gonadotropins on in vitro maturation and embryonic development, cell proliferation, and biosynthetic activity of cumulus‐oocyte complexes and granulosa cells in buffalo , 1998, Molecular reproduction and development.

[11]  C. Simón,et al.  Single blastomeres within human preimplantation embryos express different amounts of messenger ribonucleic acid for beta-actin and interleukin-1 receptor type I. , 1998, The Journal of clinical endocrinology and metabolism.

[12]  C. Wrenzycki,et al.  Expression of RNA from developmentally important genes in preimplantation bovine embryos produced in TCM supplemented with BSA. , 1998, Journal of reproduction and fertility.

[13]  P. D. De Sousa,et al.  Analysis of variation in relative mRNA abundance for specific gene transcripts in single bovine oocytes and early embryos , 1998, Molecular reproduction and development.

[14]  Z. Rosenwaks,et al.  Expression of IGFs and Their Receptors Is a Potential Marker for Embryo Quality , 1997, American journal of reproductive immunology.

[15]  G. Brem,et al.  Effect of insulin-like growth factor I (IGF-I) at high concentrations on blastocyst development of bovine embryos produced in vitro. , 1997, Journal of reproduction and fertility.

[16]  P. De Los Rios,et al.  Bovine oviductal and embryonic insulin-like growth factor binding proteins: possible regulators of "embryotrophic" insulin-like growth factor circuits. , 1997, Biology of reproduction.

[17]  R. Winston,et al.  Expression of mRNA for the insulin‐like growth factors and their receptors in human preimplantation embryos , 1997, Molecular reproduction and development.

[18]  C. Wrenzycki,et al.  Expression of the gap junction gene connexin43 (Cx43) in preimplantation bovine embryos derived in vitro or in vivo. , 1996, Journal of reproduction and fertility.

[19]  B. Brackett,et al.  In vitro developmental competence of in vitro-matured bovine oocytes fertilized and cultured in completely defined media. , 1996, Biology of reproduction.

[20]  C. Shim,et al.  Differential expression of laminin chain‐specific mRNA transcripts during mouse preimplantation embryo development , 1996, Molecular reproduction and development.

[21]  M. Hishinuma,et al.  Insulin and insulin-like growth factor-I (IGF-I) stimulate the development of bovine embryos fertilized in vitro. , 1995, The Journal of veterinary medical science.

[22]  H. Niemann,et al.  In vitro maturation, fertilization and culture to blastocysts of bovine oocytes in protein-free media. , 1995, Theriogenology.

[23]  P. Chomczyński,et al.  Substitution of chloroform by bromo-chloropropane in the single-step method of RNA isolation. , 1995, Analytical biochemistry.

[24]  G. Schultz,et al.  A growth factor phenotype map for ovine preimplantation development. , 1994, Biology of reproduction.

[25]  G. Schultz,et al.  Possible roles of insulin and insulin-like growth factors in rat preimplantation development: investigation of gene expression by reverse transcription-polymerase chain reaction. , 1994, Journal of reproduction and fertility.

[26]  R. Schultz,et al.  Igf2r and Igf2 gene expression in androgenetic, gynogenetic, and parthenogenetic preimplantation mouse embryos: absence of regulation by genomic imprinting. , 1994, Genes & development.

[27]  Prahlad T. Ram,et al.  Expression patterns of novel genes during mouse preimplantation embryogenesis , 1994, Molecular reproduction and development.

[28]  R. Schultz,et al.  Temporal pattern of IGF‐I expression during mouse preimplantation embryogenesis , 1994, Molecular reproduction and development.

[29]  Mohammad NasrEsfahani,et al.  Radical solutions and cultural problems: Could free oxygen radicals be responsible for the impaired development of preimplantation mammalian embryos in vitro? , 1994, BioEssays : news and reviews in molecular, cellular and developmental biology.

[30]  S. Heyner,et al.  Functions of the IGFs in early mammalian development , 1993, Molecular reproduction and development.

[31]  D. Rappolee,et al.  Insulin-like growth factor II acts through an endogenous growth pathway regulated by imprinting in early mouse embryos. , 1992, Genes & development.

[32]  A. Lucas-Hahn,et al.  Effects of insulin-like growth factor-I on in-vitro production of bovine embryos , 1992 .

[33]  G. Schultz,et al.  Expression of growth factor ligand and receptor genes in the preimplantation bovine embryo , 1992, Molecular reproduction and development.

[34]  A. Lucas-Hahn,et al.  Fertilization and developmental competence of bovine oocytes derived from different categories of antral follicles , 1992, Molecular reproduction and development.

[35]  D. Barlow,et al.  The mouse insulin-like growth factor type-2 receptor is imprinted and closely linked to the Tme locus , 1991, Nature.

[36]  E. Rutanen,et al.  Insulin-like growth factors and their binding proteins. , 1990, Acta endocrinologica.

[37]  A. Efstratiadis,et al.  A growth-deficiency phenotype in heterozygous mice carrying an insulin-like growth factor II gene disrupted by targeting , 1990, Nature.

[38]  F. Gannon,et al.  Nucleotide sequence of the bovine insulin-like growth factor 1 (IGF-1) and its IGF-1A precursor. , 1990, Nucleic acids research.

[39]  B. Tsang,et al.  Gonadotropins increase concentrations of immunoreactive insulin-like growth factor-I in porcine follicular fluid in vivo. , 1988, Biology of reproduction.

[40]  S. Kornfeld,et al.  Cloning and sequence analysis of the cation-independent mannose 6-phosphate receptor. , 1988, The Journal of biological chemistry.

[41]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[42]  J. Chirgwin,et al.  Cloning of the bovine 215-kDa cation-independent mannose 6-phosphate receptor. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[43]  J. Parrish,et al.  Bovine in vitro fertilization with frozen-thawed semen. , 1986, Theriogenology.

[44]  R. Easterlin.  Fertility and development. , 1980, Population bulletin of the United Nations Economic Commission for Western Asia.

[45]  H. R. Tervit,et al.  Successful culture in vitro of sheep and cattle ova. , 1972, Journal of reproduction and fertility.

[46]  J. Koppel,et al.  The influence of insulin on the in vitro development of mouse and bovine embryos. , 2000, Physiological research.

[47]  C Wrenzycki,et al.  Alterations of expression of developmentally important genes in preimplantation bovine embryos by in vitro culture conditions: implications for subsequent development. , 2000, Theriogenology.

[48]  H. R. Tervit,et al.  Exogenous protein affects developmental competence and metabolic activity of bovine pre-implantation embryos in vitro. , 1998, Reproduction, fertility, and development.

[49]  P. Kaye,et al.  The role of growth factors in preimplantation development. , 1995, Progress in growth factor research.

[50]  B. Moats-Staats,et al.  Possible autocrine/paracrine actions of insulin-like growth factors during embryonic development: expression and action of IGFs in undifferentiated P19 cells. , 1993, Developmental genetics.

[51]  G. Schultz,et al.  Insulin, insulin-like growth factors and glucose transporters: temporal patterns of gene expression in early murine and bovine embryos. , 1992, Reproduction, fertility, and development.

[52]  K. Emerson.  Endocrinology and metabolism. , 1952, Medicine of the year.