Effect of internal viral sequences on the utility of retroviral vectors

Expression of the human ADA cDNA encoded by the Moloney murine leukemia virus spliced RNA form is enhanced by intron-contained sequences. The presence of sequences corresponding to the viral gag gene in a Moloney murine leukemia virus-based vector results in the generation of 10- to 40-fold higher titers of virus.

[1]  C. Stoltzfus,et al.  Noncoding region between the env and src genes of Rous sarcoma virus influences splicing efficiency at the src gene 3' splice site , 1987, Journal of virology.

[2]  P. Kantoff,et al.  Correction of adenosine deaminase deficiency in cultured human T and B cells by retrovirus-mediated gene transfer. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[3]  A. Miller,et al.  Retroviral transfer of genes into canine hemopoietic progenitor cells in culture: a model for human gene therapy. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[4]  H. Temin,et al.  Insertion of several different DNAs in reticuloendotheliosis virus strain T suppresses transformation by reducing the amount of subgenomic mRNA , 1986, Journal of virology.

[5]  A. Miller,et al.  Retrovirus-mediated transfer and expression of drug resistance genes in human haematopoietic progenitor cells , 1986, Nature.

[6]  A. Miller,et al.  Factors involved in production of helper virus-free retrovirus vectors , 1986, Somatic cell and molecular genetics.

[7]  P. Kantoff,et al.  Gene expression in mice after high efficiency retroviral-mediated gene transfer. , 1985, Science.

[8]  G. Keller,et al.  Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursors , 1985, Nature.

[9]  J. Park,et al.  Role of intron-contained sequences in formation of moloney murine leukemia virus env mRNA , 1984, Molecular and cellular biology.

[10]  C. Cepko,et al.  Construction and applications of a highly transmissible murine retrovirus shuttle vector , 1984, Cell.

[11]  A. Joyner,et al.  Retrovirus transduction: segregation of the viral transforming function and the herpes simplex virus tk gene in infectious Friend spleen focus-forming virus thymidine kinase vectors , 1983 .

[12]  A. Joyner,et al.  Retrovirus transduction: segregation of the viral transforming function and the herpes simplex virus tk gene in infectious Friend spleen focus-forming virus thymidine kinase vectors. , 1983, Molecular and cellular biology.

[13]  J. Hutton,et al.  Cloning of cDNA sequences of human adenosine deaminase. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[14]  D. Baltimore,et al.  Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus , 1983, Cell.

[15]  W. Reznikoff,et al.  Genetic organization of Tn5. , 1981, Cold Spring Harbor symposia on quantitative biology.

[16]  R. F. Weaver,et al.  Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates. , 1979, Nucleic acids research.

[17]  P. Sharp,et al.  Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids , 1977, Cell.

[18]  H. Harris,et al.  Adenosine deaminase polymorphism in man , 1968 .