Acellular dental matrices promote functional differentiation of ameloblasts.

EDTA treatment of post-natal mouse molars made possible the isolation of cell-free dental matrices composed of basal lamina, predentin, dentin and enamel. Trypsin-isolated dental papillae and enamel organs from embryonic-mouse mandibular molars were combined with isolated matrices and cultured in vitro. In such recombinations, functional odontoblasts were never observed. On the other hand, competent preameloblasts in contact with the epithelial side of occlusal predentin overtly differentiated. Matrices treated with guanidine-EDTA or acetic acid were unable to promote the functional differentiation of ameloblasts. These data are discussed in terms of the epitheliomesenchymal interactions involved in odontogenesis.

[1]  H. Lesot,et al.  Epithelial-mesenchymal interactions in tooth germs: mechanisms of differentiation. , 1983, Journal de biologie buccale.

[2]  J. Ruch,et al.  Does the basement membrane control the mitotic activity of the inner dental epithelium of the embryonic mouse first lower molar. , 1982, Developmental biology.

[3]  H. Lesot,et al.  Facts and hypotheses concerning the control of odontoblast differentiation. , 1982, Differentiation; research in biological diversity.

[4]  J. Ruch,et al.  Behavior of Odontoblasts and Basal Lamina of Trypsin or EDTA-isolated Mouse Dental Papillae in Short-term Culture , 1981, Journal of dental research.

[5]  I. Thesleff,et al.  Tissue interactions in tooth development. , 1981, Differentiation; research in biological diversity.

[6]  H. Lesot,et al.  Immunofluorescent localization of collagens, fibronectin, and laminin during terminal differentiation of odontoblasts. , 1981, Developmental biology.

[7]  J. M. Meyer,et al.  Basement membrane reconstitution and cytodifferentiation of odontoblasts in isochronal and heterochronal reassociations of enamel organs and pulps. , 1978, Journal de biologie buccale.

[8]  E. Kallenbach,et al.  The changing morphology of the epithelium-mesenchyme interface in the differentiation zone of growing teeth of selected vertebrates and its relationship to possible mechanisms of differentiation. , 1978, Journal de biologie buccale.

[9]  L. Saxén,et al.  Basement membrane formation in transfilter tooth culture and its relation to odontoblast differentiation. , 1978, Differentiation; research in biological diversity.

[10]  H. Slavkin,et al.  Epithelial-mesenchyme interactions during odontogenesis. IV. Morphological evidence for direct heterotypic cell-cell contacts. , 1976, Developmental biology.

[11]  J. Ruch,et al.  Cell division and cytodifferentiation of odontoblasts. , 1976, Differentiation; research in biological diversity.

[12]  E. Kallenbach.  Fine structure of differentiating ameloblasts in the kitten. , 1976, The American journal of anatomy.

[13]  J. Ruch,et al.  The effects of L-azetidine-2-carboxylic acid (analogue of proline) on dental cytodifferentiations in vitro. , 1974, Differentiation; research in biological diversity.

[14]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[15]  H. Slavkin,et al.  Epithelial and mesenchymal cell interactions with extracellular matrix material in vitro. , 1969, Journal of embryology and experimental morphology.

[16]  W. E. Koch,et al.  In vitro differentiation of tooth rudiments of embryonic mice. I. Transfilter interaction of embryonic incisor tissues. , 1967, The Journal of experimental zoology.

[17]  E. Reith,et al.  The early stage of amelogenesis as observed in molar teeth of young rats. , 1967, Journal of ultrastructure research.

[18]  Robert L. Trelstad,et al.  The Role of extracellular matrix in development , 1984 .

[19]  D. Eisenmann,et al.  Ultrastructural study of the effects of fixation and fluoride injection on stippled material during amelogenesis in the rat. , 1984, Archives of oral biology.

[20]  H. Lesot,et al.  Extracellular matrix-mediated interactions during odontogenesis. , 1984, Progress in clinical and biological research.

[21]  H. Slavkin,et al.  Basal lamina persistence during epithelial-mesenchymal interactions in murine tooth development in vitro. , 1983, Journal of craniofacial genetics and developmental biology.

[22]  H. Slavkin,et al.  Molecular aspects of tooth morphogenesis and differentiation , 1981 .

[23]  J. Wolters.  The transfilter transmission of [3H]-proline labelled material in cultured rat tooth germs. , 1978, Archives of oral biology.

[24]  P. Van Mullem,et al.  Electron microscopy of epithelio-mesenchyme intercellular communication in trans-filter cultures of rat tooth germs. , 1977, Archives of oral biology.

[25]  H. Slavkin,et al.  Epithelial-mesenchymal interactions during odontogenesis II. Intercellular matrix vesicles , 1972 .