Trojan horses of the microbial world: protozoa and the survival of bacterial pathogens in the environment.

Protozoa, of which there are thousands of species, are ubiquitous in diverse natural habitats such as fresh and salt water, moist soils and even dry sands. The role of freeliving protozoa in terrestrial and aquatic environments as predators of bacteria is widely acknowledged. Predation by protozoa has a significant effect in controlling bacterial populations in soil, and the degradation of bacteria undoubtedly contributes to the maintenance of soil fertility (Foster & Dormaar, 1991 ; Weekers e t al., 1993). Likewise, protozoa play an integral part in the cycling of nutrients in aquatic food chains (Porter, 1984; Wright & Coffin, 1984).

[1]  J. Hacker,et al.  Analysis of virulence factors of Legionella pneumophila. , 1993, Zentralblatt fur Bakteriologie : international journal of medical microbiology.

[2]  J. D. de Jonckheere,et al.  Differences in destruction of cysts of pathogenic and nonpathogenic Naegleria and Acanthamoeba by chlorine , 1976, Applied and environmental microbiology.

[3]  B. Drasar,et al.  Association of Vibrio cholerae with fresh water amoebae. , 1992, Journal of medical microbiology.

[4]  G. Vogels,et al.  Effects of Grazing by the Free-Living Soil Amoebae Acanthamoeba castellanii, Acanthamoeba polyphaga, and Hartmannella vermiformis on Various Bacteria , 1993, Applied and environmental microbiology.

[5]  W. Martin,et al.  Proliferation of Legionella pneumophila as an intracellular parasite of the ciliated protozoan Tetrahymena pyriformis , 1984, Applied and environmental microbiology.

[6]  J. Kurtz,et al.  Interaction of L. pneumophila and a free living amoeba (Acanthamoeba palestinensis) , 1983, Journal of Hygiene.

[7]  S. L. Chang,et al.  Resistance of pathogenic Naegleria to some common physical and chemical agents , 1978, Applied and environmental microbiology.

[8]  G. Ware,et al.  Association of Listeria spp. Contamination in the Dairy Processing Plant Environment with the Presence of Staphylococci. , 1990, Journal of food protection.

[9]  S. Kilvington,et al.  Survival of Legionella pneumophila within cysts of Acanthamoeba polyphaga following chlorine exposure. , 1990, The Journal of applied bacteriology.

[10]  B. Singh SELECTION OF BACTERIAL FOOD BY SOIL FLAGELLATES AND AMOEBAE , 1942 .

[11]  C. W. Keevil,et al.  Biocide treatment of biofilms , 1990 .

[12]  LeVerne D. Hudson,et al.  Coliforms in a water distribution system: a remedial approach , 1983 .

[13]  S. Falkow,et al.  Coordinate regulation and sensory transduction in the control of bacterial virulence. , 1989, Science.

[14]  J. Dowling,et al.  Virulence factors of the family Legionellaceae. , 1992, Microbiological reviews.

[15]  T. Rowbotham Current views on the relationships between amoebae, legionellae and man. , 1986, Israel journal of medical sciences.

[16]  J. V. Lee,et al.  VIBRIO CHOLERÆ IN KENT , 1979, The Lancet.

[17]  Y. Tsai,et al.  Detection of Legionella species in sewage and ocean water by polymerase chain reaction, direct fluorescent-antibody, and plate culture methods , 1993, Applied and environmental microbiology.

[18]  J. Costerton,et al.  Bacterial biofilms in nature and disease. , 1987, Annual review of microbiology.

[19]  W. Drozanski Sarcobium lyticum gen. nov., sp. nov., an Obligate Intracellular Bacterial Parasite of Small Free-Living Amoebae† , 1991 .

[20]  J. Preer,et al.  Kappa and other endosymbionts in Paramecium aurelia. , 1974, Bacteriological reviews.

[21]  T. Brown,et al.  Viability of pathogenic and nonpathogenic free-living amoebae in long-term storage at a range of temperatures , 1984, Applied and environmental microbiology.

[22]  R. Bhopal,et al.  Legionnaires' disease: the infective dose paradox , 1993, The Lancet.

[23]  H. Müller,et al.  INTERACTIONS OF LISTERIA MONOCYTOGENES, LISTERIA SEELIGERI, AND LISTERIA INNOCUA WITH PROTOZOANS , 1990 .

[24]  D. S. Katz,et al.  Free-Living Amoebae Used to Isolate Consortia Capable of Degrading Trichloroethylene , 1991, Applied biochemistry and biotechnology.

[25]  C. Mody,et al.  A Legionella pneumophila gene encoding a species-specific surface protein potentiates initiation of intracellular infection , 1989, Infection and immunity.

[26]  R. Hodinka,et al.  Ultrastructural study of mode of entry of Chlamydia psittaci into L-929 cells , 1986, Infection and immunity.

[27]  H. Müller,et al.  Ingested Listeria monocytogenes survive and multiply in protozoa. , 1990, Journal of medical microbiology.

[28]  J. Hall,et al.  Bacterial endosymbionts of Acanthamoeba sp. , 1985, The Journal of parasitology.

[29]  T. Embley,et al.  Direct amplification and sequencing of the 16S ribosomal DNA of an intracellular Legionella species recovered by amoebal enrichment from the sputum of a patient with pneumonia. , 1991, FEMS microbiology letters.

[30]  Christopher H. King,et al.  Enhancement of Edwardsiella tarda and Aeromonas salmonicida through ingestion by the ciliated protozoan Tetrahymena pyriformis , 1988 .

[31]  M. R. Brown,et al.  Influence of growth rate on susceptibility to antimicrobial agents: biofilms, cell cycle, dormancy, and stringent response , 1990, Antimicrobial Agents and Chemotherapy.

[32]  T. Fritsche,et al.  Occurrence of bacterial endosymbionts in Acanthamoeba spp. isolated from corneal and environmental specimens and contact lenses , 1993, Journal of clinical microbiology.

[33]  J. Sýkora,et al.  Growth-supporting activity for Legionella pneumophila in tap water cultures and implication of hartmannellid amoebae as growth factors , 1988, Applied and environmental microbiology.

[34]  N. Cheville,et al.  Entry and Intracellular Localization of Brucella spp. in Vero Cells: Fluorescence and Electron Microscopy , 1990, Veterinary pathology.

[35]  T. Rowbotham,et al.  Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae. , 1980, Journal of clinical pathology.

[36]  Marc A. Goshko,et al.  Coliform occurrence and chlorine residual in small water distribution systems , 1983 .

[37]  M. R. Brown,et al.  Relationship between Legionella pneumophila and Acanthamoeba polyphaga: Physiological Status and Susceptibility to Chemical Inactivation , 1992, Applied and environmental microbiology.

[38]  M. Alexander Why microbial predators and parasites do not eliminate their prey and hosts. , 1981, Annual review of microbiology.

[39]  N. Cianciotto,et al.  Legionella pneumophila mip gene potentiates intracellular infection of protozoa and human macrophages. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[40]  J. Frank,et al.  Susceptibility of Starved Planktonic and Biofilm Listeria monocytogenes to Quaternary Ammonium Sanitizer as Determined by Direct Viable and Agar Plate Counts. , 1993, Journal of food protection.

[41]  M. Alexander,et al.  Regulation of predation by prey density: the protozoan-Rhizobium relationship. , 1975, Applied microbiology.

[42]  J. Barbaree,et al.  Virulence of a Legionella anisa strain associated with Pontiac fever: an evaluation using protozoan, cell culture, and guinea pig models , 1990, Infection and immunity.

[43]  S. Kilvington Activity of water biocide chemicals and contact lens disinfectants on pathogenic free-living amoebae , 1990 .

[44]  M. Costa,et al.  Biofilms as major sources of Legionella spp. in hydrothermal areas and their dispersion into stream water , 1993 .

[45]  W. S. Mauchline Environmental regulation of the growth, physiology and virulence of Legionella pneumophila , 1996 .

[46]  E. White,et al.  Effects of cytochalasin D and methylamine on intracellular growth of Legionella pneumophila in amoebae and human monocyte-like cells , 1991, Infection and immunity.

[47]  B. Singh A method of estimating the numbers of soil protozoa, especially amoebae, based on their differential feeding on bacteria. , 1946, The Annals of applied biology.

[48]  R. Colwell,et al.  Vibrio cholerae, Vibrio parahaemolyticus, and other vibrios: occurrence and distribution in Chesapeake Bay. , 1977, Science.

[49]  M. R. Brown,et al.  Sensitivity of biofilms to antimicrobial agents. , 1993, The Journal of applied bacteriology.

[50]  N. Cianciotto,et al.  Nucleotide sequence analysis of the Legionella micdadei mip gene, encoding a 30-kilodalton analog of the Legionella pneumophila Mip protein , 1991, Infection and immunity.

[51]  M. R. Brown,et al.  The influence of environment on envelope properties affecting survival of bacteria in infections. , 1985, Annual review of microbiology.

[52]  E. Shotts,et al.  Survival of coliforms and bacterial pathogens within protozoa during chlorination , 1988, Applied and environmental microbiology.

[53]  P. Lambert,et al.  Influence of intra-amoebic and other growth conditions on the surface properties of Legionella pneumophila , 1993, Infection and immunity.

[54]  D. Warhurst,et al.  Isolation of amoebae from the air. , 1969, Journal of medical microbiology.

[55]  I. R. Richardson The incidence of Bdellovibrio spp. in man-made water systems: coexistence with legionellas. , 1990, The Journal of applied bacteriology.

[56]  R. Colwell,et al.  Isolation of amoebae and Pseudomonas and Legionella spp. from eyewash stations , 1991, Applied and environmental microbiology.