Imaging H217O distribution in a phantom and measurement of metabolically produced H217O in live mice by proton NMR

Previously we suggested a method for indirect detection of H217O via a combination of 1H spin–echo sequence and 17O decoupling. The method is based on the shortening of the water proton T2 due to the residual spin–spin coupling with the 17O nucleus, and on our finding that this shortening is abolished by applying an radio frequency field at the 17O resonance frequency. In the present work we demonstrate how the method can be used for imaging low concentrations of H217O, and report for the first time an application of this method to in vivo detection of H217O in live newborn mice, where the H217O was introduced either directly i.p. or by inhalation of 17O2 where it was formed by oxidative metabolic activity. © 1997 John Wiley & Sons, Ltd.

[1]  Keith R. Thulborn,et al.  Proton imaging for in vivo blood flow and oxygen consumption measurements , 1981 .

[2]  C T Moonen,et al.  In vivo 17O NMR study of rat brain during 17O2 inhalation , 1992, Magnetic resonance in medicine.

[3]  G. Navon,et al.  A new method for proton detection of H217O with potential applications for functional MRI , 1994, Magnetic resonance in medicine.

[4]  Noninvasive determination of regional cerebral blood flow in rats using dynamic imaging with Gd(DTPA) , 1991, Magnetic resonance in medicine.

[5]  A. Haase,et al.  Rapid NMR Imaging Using Low Flip-Angle Pulses , 2004 .

[6]  B. Rosen,et al.  Proton NMR imaging of cerebral blood flow using H2 17O , 1991, Magnetic resonance in medicine.

[7]  R. Reddy,et al.  Detection of 17O by Proton T1ρ Dispersion Imaging , 1995 .

[8]  R. Turner,et al.  Dynamic magnetic resonance imaging of human brain activity during primary sensory stimulation. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[9]  A. Hopkins,et al.  Oxygen‐17 compounds as potential NMR T2 contrast agents: Enrichment effects of H217O on protein solutions and living tissues , 1987, Magnetic resonance in medicine.

[10]  B. Rosen,et al.  Perfusion imaging with NMR contrast agents , 1990, Magnetic resonance in medicine.

[11]  D. Fiat,et al.  Determination of the rate of cerebral oxygen consumption and regional cerebral blood flow by non-invasive 17O in vivo NMR spectroscopy and magnetic resonance imaging: Part 1. Theory and data analysis methods. , 1992, Neurological research.

[12]  Ravi S. Menon,et al.  Intrinsic signal changes accompanying sensory stimulation: functional brain mapping with magnetic resonance imaging. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[13]  K Kito,et al.  In vivo oxygen-17 nuclear magnetic resonance for the estimation of cerebral blood flow and oxygen consumption. , 1991, Biochemical and biophysical research communications.

[14]  J. Pekar,et al.  In Vivo measurement of cerebral oxygen consumption and blood flow using 17O magnetic resonance imaging , 1991, Magnetic resonance in medicine.

[15]  S. Ogawa,et al.  Oxygenation‐sensitive contrast in magnetic resonance image of rodent brain at high magnetic fields , 1990, Magnetic resonance in medicine.

[16]  B. Rosen,et al.  Functional mapping of the human visual cortex by magnetic resonance imaging. , 1991, Science.

[17]  E M Haacke,et al.  Improved sensitivity of proton MR to oxygen‐17 as a contrast agent using fast imaging: Detection in brain , 1988, Magnetic resonance in medicine.

[18]  J A Frank,et al.  Simultaneous Measurement of Cerebral Oxygen Consumption and Blood Flow Using 17O and 19F Magnetic Resonance Imaging , 1995, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[19]  Peter Mansfield,et al.  Planar spin imaging by NMR , 1976 .

[20]  P. Wielopolski,et al.  The stability of proton T2 effects of oxygen‐17 water in experimental cerebral ischemia , 1991, Magnetic resonance in medicine.

[21]  K. Ishimori,et al.  Cerebral oxygen utilization analyzed by the use of oxygen-17 and its nuclear magnetic resonance. , 1990, Biochemical and biophysical research communications.

[22]  S. Meiboom,et al.  NUCLEAR MAGNETIC RESONANCE STUDY OF THE PROTON TRANSFER IN WATER , 1961 .