The phagocytosis of biodegradable albumin (Alb) microspheres by peripheral blood cells (neutrophils and monocytes) and mouse macrophage cells (peritoneal and lung macrophages) as a carrier matrix in drug targeting applications was investigated. Glutaraldehyde crosslinking was used to produce albumin microspheres with different sizes. Plain (nonmodified) microspheres with diameters of1, 2, and 4 mm were cultured with cells and their uptake was determined as 8, 5, and 1 particles/cell, respectively. Albumin microspheres (2 mm diameter) were also modified with various opsonization and passivation agents, such as fibronectin (Fn), amicasine, and poly(ethylene glycol) (PEG). Plain, Alb-PEG, Alb-Fn, and Alb-amicasine microspheres were phagocytized by monocytes and neutrophils and the number of particles taken up by each cell significantly increased for Alb-Fn microspheres with respect to the plain microspheres. No significant phagocytosis was observed for Alb-PEG microspheres. The number of particles taken up by each cell was less than the plain and amikacin modified albumin microspheres. Almost zero uptake was obtained for the phagocytosis of Alb-PEG microspheres with lung and peritoneal macrophages. For phagocytosis of plain and other modified particles by the macrophages, although the number of particles taken up by each cell increased with respect to those with neutrophils and monocytes, similar tendencies were also obtained.
[1]
Y Ikada,et al.
Effect of the size and surface charge of polymer microspheres on their phagocytosis by macrophage.
,
1988,
Biomaterials.
[2]
Y. Ohtsuka,et al.
Phagocytosis of latex particles by leucocytes. I. Dependence of phagocytosis on the size and surface potential of particles.
,
1986,
Biomaterials.
[3]
H. Walter,et al.
Factors in the partition of red blood cells in aqueous dextran-polyethylene glycol two-phase systems.
,
1968,
Biochimica et biophysica acta.
[4]
C. J. Oss,et al.
Phagocytosis as a Surface Phenomenon
,
1978
.
[5]
Y. Ikada,et al.
Macrophage phagocytosis of biodegradable microspheres composed of L-lactic acid/glycolic acid homo- and copolymers.
,
1988,
Journal of biomedical materials research.
[6]
H. Kawaguchi,et al.
Modifications of a polymer latex
,
1984
.
[7]
Y. Ikada,et al.
Macrophage activation through phagocytosis of muramyl dipeptide encapsulated in gelatin microspheres
,
1987,
The Journal of pharmacy and pharmacology.