Phosphate-assisted phytoremediation of arsenic by Brassica napus and Brassica juncea: Morphological and physiological response

ABSTRACT In this study, we examined the potential role of phosphate (P; 0, 50, 100 mg kg−1) on growth, gas exchange attributes, and photosynthetic pigments of Brassica napus and Brassica juncea under arsenic (As) stress (0, 25, 50, 75 mg kg−1) in a pot experiment. Results revealed that phosphate supplementation (P100) to As-stressed plants significantly increased shoot As concentration, dry biomass yield, and As uptake, in addition to the improved morphological and gas exchange attributes and photosynthetic pigments over P0. However, phosphate-assisted increase in As uptake was substantially (up to two times) greater for B. napus, notably due to higher shoot As concentration and dry biomass yield, compared to B. juncea at the P100 level. While phosphate addition in soil (P100) led to enhanced shoot As concentration in B. juncea, it reduced shoot dry biomass, primarily after 50 and 75 mg kg−1 As treatments. The translocation factor and bioconcentration factor values of B. napus were higher than B. juncea for all As levels in the presence of phosphate. This study demonstrates that phosphate supplementation has a potential to improve As phytoextraction efficiency, predominantly for B. napus, by minimizing As-induced damage to plant growth, as well as by improving the physiological and photosynthetic attributes.

[1]  Amit Kumar,et al.  Selenate mitigates arsenite toxicity in rice (Oryza sativa L.) by reducing arsenic uptake and ameliorates amino acid content and thiol metabolism. , 2016, Ecotoxicology and environmental safety.

[2]  N. Niazi,et al.  Arsenic sorption to nanoparticulate mackinawite (FeS): An examination of phosphate competition. , 2016, Environmental pollution.

[3]  M. Bernal,et al.  Arsenic(V) adsorption-desorption in agricultural and mine soils: Effects of organic matter addition and phosphate competition. , 2016, Environmental pollution.

[4]  P. Suprasanna,et al.  Arsenic toxicity in rice (Oryza sativa L.) is influenced by sulfur supply: Impact on the expression of transporters and thiol metabolism , 2016 .

[5]  L. Ma,et al.  Arsenic-induced plant growth of arsenic-hyperaccumulator Pteris vittata: Impact of arsenic and phosphate rock. , 2016, Chemosphere.

[6]  N. Bolan,et al.  Remediation of arsenic-contaminated water using agricultural wastes as biosorbents , 2016 .

[7]  O. Dhankher,et al.  Nitric Oxide Alleviated Arsenic Toxicity by Modulation of Antioxidants and Thiol Metabolism in Rice (Oryza sativa L.) , 2016, Front. Plant Sci..

[8]  R. Naidu,et al.  Unraveling Health Risk and Speciation of Arsenic from Groundwater in Rural Areas of Punjab, Pakistan , 2015, International journal of environmental research and public health.

[9]  L. Ma,et al.  Phosphorus solubilization and plant growth enhancement by arsenic-resistant bacteria. , 2015, Chemosphere.

[10]  Budiman Minasny,et al.  Mid-infrared spectroscopy and partial least-squares regression to estimate soil arsenic at a highly variable arsenic-contaminated site , 2015, International Journal of Environmental Science and Technology.

[11]  S. Hussain,et al.  Citric acid improves lead (pb) phytoextraction in brassica napus L. by mitigating pb-induced morphological and biochemical damages. , 2014, Ecotoxicology and environmental safety.

[12]  N. Iqbal,et al.  Citric acid assisted phytoremediation of copper by Brassica napus L. , 2014, Ecotoxicology and environmental safety.

[13]  Tiantian Xiong,et al.  Water extraction kinetics of metals, arsenic and dissolved organic carbon from industrial contaminated poplar leaves. , 2013, Journal of environmental sciences.

[14]  N. Bolan,et al.  Phosphorus-arsenic interactions in variable-charge soils in relation to arsenic mobility and bioavailability. , 2013, The Science of the total environment.

[15]  L. Guilherme,et al.  Phosphorus Improves Arsenic Phytoremediation by Anadenanthera Peregrina by Alleviating Induced Oxidative Stress , 2013, International journal of phytoremediation.

[16]  L. Ma,et al.  Sparingly-soluble phosphate rock induced significant plant growth and arsenic uptake by Pteris vittata from three contaminated soils. , 2013, Environmental science & technology.

[17]  S. Sinha,et al.  Co-application of selenite and phosphate reduces arsenite uptake in hydroponically grown rice seedlings: toxicity and defence mechanism. , 2013, Ecotoxicology and environmental safety.

[18]  G. Owens,et al.  Screening Indian Mustard Genotypes for Phytoremediating Arsenic-Contaminated Soils , 2013 .

[19]  A. Karczewska,et al.  INFLUENCE OF SOIL PROPERTIES AND PHOSPHATE ADDITION ON ARSENIC UPTAKE FROM POLLUTED SOILS BY VELVETGRASS (HOLCUS LANATUS) , 2013, International journal of phytoremediation.

[20]  E. Codling.  PHOSPHORUS AND ARSENIC UPTAKE BY CORN, WHEAT AND SOYBEAN FROM BROILER LITTER ASH AND EGG LAYER MANURE ASH , 2013 .

[21]  L. Krumholz,et al.  Exposure of Soil Microbial Communities to Chromium and Arsenic Alters Their Diversity and Structure , 2012, PloS one.

[22]  L. Zwieten,et al.  Phytoremediation of an arsenic-contaminated site using Pteris vittata L. and Pityrogramma calomelanos var. austroamericana: a long-term study , 2012, Environmental Science and Pollution Research.

[23]  I. Williams,et al.  Genetic effect on phytoaccumulation of arsenic in Brassica juncea L , 2012, Euphytica.

[24]  A. Patra,et al.  Phytoremediation of Arsenic Contaminated Soil by Pteris Vittata L. II. Effect on Arsenic Uptake and Rice Yield , 2012, International journal of phytoremediation.

[25]  Balwant Singh,et al.  Arsenic speciation and phytoavailability in contaminated soils using a sequential extraction procedure and XANES spectroscopy. , 2011, Environmental science & technology.

[26]  Balwant Singh,et al.  Phytoremediation Potential of Pityrogramma Calomelanos Var. Austroamericana and Pteris Vittata L. Grown at a Highly Variable Arsenic Contaminated Site , 2011, International journal of phytoremediation.

[27]  T. Vamerali,et al.  In situ phytoremediation of arsenic- and metal-polluted pyrite waste with field crops: effects of soil management. , 2011, Chemosphere.

[28]  George E. Rayment,et al.  Soil Chemical Methods – Australasia , 2011 .

[29]  Sally E. Smith,et al.  Arsenic uptake and toxicity in plants: integrating mycorrhizal influences , 2010, Plant and Soil.

[30]  A. Juhasz,et al.  Arsenic uptake and speciation in vegetables grown under greenhouse conditions , 2009, Environmental geochemistry and health.

[31]  A. Meharg,et al.  Influence of Phosphate on the Arsenic Uptake by Wheat (Triticum durum L.) Irrigated with Arsenic Solutions at Three Different Concentrations , 2009 .

[32]  Balwant Singh,et al.  Arsenic hyperaccumulation and localization in the pinnule and stipe tissues of the gold-dust fern (Pityrogramma calomelanos (L.) Link var. austroamericana (Domin) Farw.) using quantitative micro-PIXE spectroscopy , 2007, Plant and Soil.

[33]  C. Tu,et al.  Phytoremediation of an Arsenic-Contaminated Site Using Pteris vittata L.: A Two-Year Study , 2006, International journal of phytoremediation.

[34]  Z. Rengel,et al.  Phosphate and arsenate interactions in the rhizosphere of canola (Brassica napus). , 2004, Functional plant biology : FPB.

[35]  L. Ma,et al.  Effects of compost and phosphate on plant arsenic accumulation from soils near pressure-treated wood. , 2004, Environmental pollution.

[36]  L. Ma,et al.  Effects of compost and phosphate amendments on arsenic mobility in soils and arsenic uptake by the hyperaccumulator, Pteris vittata L. , 2003, Environmental pollution.

[37]  D. Butcher,et al.  Phytoremediation of Arsenic and Lead in Contaminated Soil Using Chinese Brake Ferns (Pteris vittata) and Indian Mustard (Brassica juncea) , 2003, International journal of phytoremediation.

[38]  S. McGrath,et al.  Arsenic hyperaccumulation by different fern species , 2002 .

[39]  P. Smedley,et al.  A review of the source, behaviour and distribution of arsenic in natural waters , 2002 .

[40]  A. Meharg,et al.  Arsenic uptake and metabolism in arsenic resistant and nonresistant plant species , 2002 .

[41]  C. Tu,et al.  Effects of arsenic concentrations and forms on arsenic uptake by the hyperaccumulator ladder brake. , 2002, Journal of environmental quality.

[42]  Domy C. Adriano,et al.  Trace Elements in Terrestrial Environments: Biogeochemistry, Bioavailability, and Risks of Metals , 2001 .

[43]  A. Hegedűs,et al.  Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedlings under cadmium stress , 2001 .

[44]  Yong Cai,et al.  A fern that hyperaccumulates arsenic , 2001, Nature.

[45]  H. Schat,et al.  TOXICITY OF ARSENATE IN SILENE VULGARIS, ACCUMULATION AND DEGRADATION OF ARSENATE-INDUCED PHYTOCHELATINS , 1999 .

[46]  R. Miller.  Nitric-perchloric Acid Wet Digestion In An Open Vessel , 1997 .

[47]  B. Singh,et al.  Effect of different forms and sources of arsenic on crop yield and arsenic concentration , 1994, Water, Air, and Soil Pollution.

[48]  H. Metzner,et al.  Untersuchungen zur Synchronisierbarkeit einzelner Pigmentmangel-Mutanten von Chlorella , 1965, Planta.

[49]  M. Nawaz,et al.  Phytoremediation of Arsenic-Contaminated Soils Using Arsenic Hyperaccumulating Ferns , 2016 .

[50]  Muhammad Bilal Shakoor,et al.  Citric acid assisted phytoremediation of cadmium by Brassica napus L. , 2014, Ecotoxicology and environmental safety.

[51]  A. Ulery,et al.  Managing Arsenic in the Environment—From Soil to Human Health , 2007 .

[52]  R. Naidu,et al.  Management of arsenic-contaminated soils , 2006 .

[53]  T. Balakhnina,et al.  The effect of cadmium on CO2 exchange, variable fluorescence of chlorophyll, and the level of antioxidant enzymes in pea leaves , 2005, Russian Journal of Plant Physiology.

[54]  N. Bolan,et al.  Chromium contamination and its risk management in complex environmental settings , 2005 .

[55]  M. R. Noll.  Trace Elements in Terrestrial Environments , 2002 .

[56]  D. Adriano.  Trace elements in terrestrial environments , 2001 .

[57]  H. Lichtenthaler.  CHLOROPHYLL AND CAROTENOIDS: PIGMENTS OF PHOTOSYNTHETIC BIOMEMBRANES , 1987 .

[58]  S. R. Olsen,et al.  Estimation of available phosphorus in soils by extraction with sodium bicarbonate , 1954 .