Spectral effects of supplementary lighting on the secondary metabolites in roses, chrysanthemums, and campanulas.

To investigate the effect of the light spectrum on photosynthesis, growth, and secondary metabolites Rosa hybrida 'Scarlet', Chrysanthemum morifolium 'Coral Charm', and Campanula portenschlagiana 'BluOne' were grown at 24/18°C day/night temperature under purpose-built LED arrays yielding approximately 200 μmol m(-2)s(-1) at plant height for 16 h per day. The four light treatments were (1) 40% Blue/60% Red, (2) 20% Blue/80% Red, (3) 100% Red, and (4) 100% White (Control). The plant height was smallest in 40% Blue/60% Red in roses and chrysanthemums, while the biomass was smallest in the white control in roses and in 100% Red in chrysanthemums. The total biomass was unaffected by the spectrum in campanulas, while the leaf area was smallest in the 40% Blue/60% Red treatment. In 100% Red curled leaves and other morphological abnormalities were observed. Increasing the blue to red ratio increased the stomatal conductance though net photosynthesis was unaffected, indicating excess stomatal conductance in some treatments. With higher blue light ratio all phenolic acids and flavonoids increased. In view of the roles of these secondary metabolites as antioxidants, anti-pathogens, and light protectants, we hypothesize that blue light may predispose plants to better cope with stress.

[1]  Shin-Ichiro Inoue,et al.  Leaf positioning of Arabidopsis in response to blue light. , 2008, Molecular plant.

[2]  Gareth I Jenkins,et al.  Signal transduction in responses to UV-B radiation. , 2009, Annual review of plant biology.

[3]  C. Fankhauser,et al.  Light-regulated plant growth and development. , 2010, Current topics in developmental biology.

[4]  Kee-Yoeup Paek,et al.  Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro , 2004 .

[5]  E. Ploschuk,et al.  Phototropins But Not Cryptochromes Mediate the Blue Light-Specific Promotion of Stomatal Conductance, While Both Enhance Photosynthesis and Transpiration under Full Sunlight12[C][W][OA] , 2011, Plant Physiology.

[6]  C. Kubota,et al.  Effects of supplemental light quality on growth and phytochemicals of baby leaf lettuce , 2009 .

[7]  Harry Smith,et al.  Phytochromes and light signal perception by plants—an emerging synthesis , 2000, Nature.

[8]  J. Olsen,et al.  EFFECTS OF LIGHT QUALITY UNDER RED AND BLUE LIGHT EMITTING DIODES ON GROWTH AND EXPRESSION OF FBP28 IN PETUNIA , 2011 .

[9]  Chentao Lin,et al.  Cryptochrome structure and signal transduction. , 2003, Annual review of plant biology.

[10]  R. Morrow.  LED Lighting in Horticulture , 2008 .

[11]  Hendrik Poorter,et al.  Blue light dose–responses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown under different combinations of red and blue light , 2010, Journal of experimental botany.

[12]  D. Kopsell,et al.  Increases in Shoot Tissue Pigments, Glucosinolates, and Mineral Elements in Sprouting Broccoli after Exposure to Short-duration Blue Light from Light Emitting Diodes , 2013 .

[13]  C. Fankhauser,et al.  Higher plants use LOV to perceive blue light. , 2009, Current opinion in plant biology.

[14]  K. Folta,et al.  Primary Inhibition of Hypocotyl Growth and Phototropism Depend Differently on Phototropin-Mediated Increases in Cytoplasmic Calcium Induced by Blue Light1 , 2003, Plant Physiology.

[15]  Chentao Lin,et al.  The action mechanisms of plant cryptochromes. , 2011, Trends in plant science.

[16]  Xing Wang Deng,et al.  Light-regulated transcriptional networks in higher plants , 2007, Nature Reviews Genetics.

[17]  C. Fankhauser,et al.  The Arabidopsis PHYTOCHROME KINASE SUBSTRATE2 Protein Is a Phototropin Signaling Element That Regulates Leaf Flattening and Leaf Positioning1[W][OA] , 2010, Plant Physiology.

[18]  T. Dougher,et al.  Differences in the Response of Wheat, Soybean and Lettuce to Reduced Blue Radiation¶ , 2001, Photochemistry and photobiology.

[19]  A. Sæbø,et al.  Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro , 1995, Plant Cell, Tissue and Organ Culture.

[20]  Marcel A K Jansen,et al.  UV-B exposure, ROS, and stress: inseparable companions or loosely linked associates? , 2013, Trends in plant science.

[21]  A. Zukauskas,et al.  OPTIMIZATION OF LIGHTING SPECTRUM FOR PHOTOSYNTHETIC SYSTEM AND PRODUCTIVITY OF LETTUCE BY USING LIGHT-EMITTING DIODES , 2006 .

[22]  D. Seigler.  Plant Secondary Metabolism , 1998, Springer US.

[23]  R M Wheeler,et al.  Improving spinach, radish, and lettuce growth under red light-emitting diodes (LEDs) with blue light supplementation. , 2001, HortScience : a publication of the American Society for Horticultural Science.

[24]  A. Cashmore,et al.  Cryptochromes: blue light receptors for plants and animals. , 1999, Science.

[25]  K. H. Kjaer,et al.  Threshold response of stomatal closing ability to leaf abscisic acid concentration during growth , 2014, Journal of experimental botany.

[26]  J. Harbinson,et al.  The responses of light interception, photosynthesis and fruit yield of cucumber to LED-lighting within the canopy. , 2010, Physiologia plantarum.

[27]  Masahiro Kasahara,et al.  Arabidopsis nph1 and npl1: Blue light receptors that mediate both phototropism and chloroplast relocation , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[28]  A. Nagatani,et al.  Tissue-Autonomous Promotion of Palisade Cell Development by Phototropin 2 in Arabidopsis[W] , 2011, Plant Cell.

[29]  Gareth I Jenkins,et al.  UV-B Promotes Rapid Nuclear Translocation of the Arabidopsis UV-B–Specific Signaling Component UVR8 and Activates Its Function in the Nucleus , 2007, The Plant Cell Online.

[30]  N. Palavan-Unsal,et al.  Phenolic Cycle in Plants and Environment , 2011 .

[31]  H. R. Gislerød,et al.  A high proportion of blue light increases the photosynthesis capacity and leaf formation rate of Rosa × hybrida but does not affect time to flower opening. , 2013, Physiologia plantarum.

[32]  Cary A. Mitchell,et al.  Plant Productivity in Response to LED Lighting , 2008 .

[33]  A. Brazaitytė,et al.  LED illumination affects bioactive compounds in romaine baby leaf lettuce. , 2013, Journal of the science of food and agriculture.

[34]  P.H.B. de Visser,et al.  Modelling Dry Matter Production and Partitioning in Sweet Pepper , 2006 .

[35]  Jihong Liu Clarke,et al.  Artificial light from light emitting diodes (LEDs) with a high portion of blue light results in shorter poinsettias compared to high pressure sodium (HPS) lamps , 2012 .

[36]  D. Fanourakis,et al.  Co-ordination of hydraulic and stomatal conductances across light qualities in cucumber leaves , 2011, Journal of experimental botany.

[37]  B. Bauer,et al.  THE INFLUENCE OF LIGHT QUALITY ON ADAPTATION AND FUNCTION OF THE PHOTOSYNTHETIC APPARATUS , 1987 .

[38]  J. Madlung,et al.  Regulation of enzymes involved in anthocyanin biosynthesis in carrot cell cultures in response to treatment with ultraviolet light and fungal elicitors , 1998, Planta.

[39]  Changhoo Chun,et al.  Effects of Supplementary LED Lighting on Growth and Biochemical Parameters in Dieffenbachia amoena ‘Camella’ and Ficus elastica ‘Melany’ , 2010 .

[40]  M. Lefsrud,et al.  Variation in Lutein, β-carotene, and Chlorophyll Concentrations among Brassica oleracea Cultigens and Seasons , 2004 .

[41]  P. Decker,et al.  Photobodies in Light Signaling1 , 2011, Plant Physiology.

[42]  M. Wink.  Functions and biotechnology of plant secondary metabolites , 2010 .

[43]  Silicon-Induced Changes in Antifungal Phenolic Acids, Flavonoids, and Key Phenylpropanoid Pathway Genes during the Interaction between Miniature Roses and the Biotrophic Pathogen Podosphaera pannosa1[W] , 2011, Plant Physiology.

[44]  M. Johkan,et al.  Blue Light-emitting Diode Light Irradiation of Seedlings Improves Seedling Quality and Growth after Transplanting in Red Leaf Lettuce , 2010 .

[45]  G. Bernier,et al.  Sucrose increase during floral induction in the phloem sap collected at the apical part of the shoot of the long-day plant Sinapis alba L. , 1993, Planta.

[46]  Meng Chen,et al.  Light signal transduction in higher plants. , 2004, Annual review of genetics.

[47]  A. Nagatani,et al.  Antagonistic regulation of leaf flattening by phytochrome B and phototropin in Arabidopsis thaliana. , 2013, Plant & cell physiology.

[48]  Karen J. Halliday,et al.  Light and Plant Development , 2007 .

[49]  D. Lynn,et al.  PHENOLIC SIGNALS IN COHABITATION: IMPLICATIONS FOR PLANT DEVELOPMENT , 1990 .

[50]  K. Gindro,et al.  Delta-viniferin, a resveratrol dehydrodimer: one of the major stilbenes synthesized by stressed grapevine leaves. , 2003, Journal of agricultural and food chemistry.

[51]  S. S. Costa,et al.  Increased Antioxidant Activity and Changes in Phenolic Profile of Kalanchoe pinnata (Lamarck) Persoon (Crassulaceae) Specimens Grown Under Supplemental Blue Light , 2013, Photochemistry and photobiology.

[52]  V. Lattanzio,et al.  Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. , 2006 .

[53]  R. Paradiso,et al.  Spectral dependence of photosynthesis and light absorptance in single leaves and canopy in rose , 2011 .

[54]  S. Kay,et al.  FKF1 is essential for photoperiodic-specific light signalling in Arabidopsis , 2003, Nature.