Candidatus Rickettsia Vini DNA in Ticks Collected from Nest Burrows of the European Sand Martin (Riparia riparia) in Sweden.

Background: Birds can cross geographical and environmental barriers and thereby facilitate dispersal of tick-borne pathogens both as carriers of infected ticks and as reservoirs of pathogenic microorganisms. Ixodes lividus (Ixodida: Ixodidae) is an endophilic tick in the Palearctic region that is highly specialized on its host, the European sand martin Riparia riparia. The purpose of this study was to determine whether I. lividus ticks sampled from sand martin nests in Sweden carry vector-borne pathogens. Materials and Methods: Fed ticks were collected in the autumns of 2017 and 2019 from the nests of a European sand martin colony in southern Sweden. Ticks were identified morphologically to developmental stage and species and were tested for tick-borne pathogens using PCR-based methods. Results: None of the 41 ticks tested positive for five tick-borne pathogens including Borrelia spp., tick-borne encephalitis virus (TBEV), Neoehrlichia mikurensis, Anaplasma phagocytophilum, and Babesia spp. Thirty-seven (13 females, 23 nymphs and 1 larva) of the 41 ticks tested positive for the gltA gene of Rickettsia spp. The sequences of the 17 kDa and gltA genes were most closely related to Candidatus Rickettsia vini. Conclusion: Our study confirms other reports that I. lividus ticks associated with the European sand martin have high infection prevalence of Ca. R. vini.

[1]  A. Sándor,et al.  Hard ticks (Acari: Ixodidae) associated with birds in Europe: Review of literature data , 2022, Frontiers in Veterinary Science.

[2]  L. Chitimia-Dobler,et al.  Rickettsia spp. in ticks (Acari: Ixodidae) from wild birds: First detection of Candidatus Rickettsia vini in Hesse, Germany. , 2022, Ticks and tick-borne diseases.

[3]  P. Wilhelmsson,et al.  Are other tick-borne infections overlooked in patients investigated for Lyme neuroborreliosis? A large retrospective study from South-eastern Sweden. , 2021, Ticks and tick-borne diseases.

[4]  E. Matthysen,et al.  Isolation of Candidatus Rickettsia vini from Belgian Ixodes arboricola ticks and propagation in tick cell lines , 2020, Ticks and tick-borne diseases.

[5]  A. Jenkins,et al.  Tick-borne pathogens in Ixodes ricinus ticks collected from migratory birds in southern Norway , 2020, PloS one.

[6]  K. Thorup,et al.  Screening for multiple tick-borne pathogens in Ixodes ricinus ticks from birds in Denmark during spring and autumn migration seasons. , 2019, Ticks and tick-borne diseases.

[7]  Å. Lundkvist,et al.  Alkhurma Hemorrhagic Fever Virus RNA in Hyalomma rufipes Ticks Infesting Migratory Birds, Europe and Asia Minor , 2018, Emerging infectious diseases.

[8]  P. Heneberg,et al.  Isolated populations of Ixodes lividus ticks in the Czech Republic and Belgium host genetically homogeneous Rickettsia vini. , 2018, Ticks and tick-borne diseases.

[9]  A. Paulauskas,et al.  First Records of Ixodes lividus from Sand Martin (Riparia riparia) Nests in Lithuania , 2017, Journal of Vector Ecology.

[10]  J. Waldenström,et al.  Co-infection with Babesia divergens and Anaplasma phagocytophilum in cattle (Bos taurus), Sweden. , 2017, Ticks and tick-borne diseases.

[11]  M. Labruna,et al.  Rickettsia vini n. sp. (Rickettsiaceae) infecting the tick Ixodes arboricola (Acari: Ixodidae) , 2016, Parasites & Vectors.

[12]  A. Giangaspero,et al.  Arthropods and associated arthropod-borne diseases transmitted by migrating birds. The case of ticks and tick-borne pathogens. , 2015, Veterinary parasitology.

[13]  A. Matussek,et al.  Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA , 2015, BMC Microbiology.

[14]  M. Labruna,et al.  Molecular characterization of 'Candidatus Rickettsia vini' in Ixodes arboricola from the Czech Republic and Slovakia. , 2015, Ticks and tick-borne diseases.

[15]  J. Oteo,et al.  Prevalence of ‘Candidatus Rickettsia vini’ in Ixodes arboricola ticks in the North of Spain, 2011–2013 , 2015, Parasites & Vectors.

[16]  Å. Andreassen,et al.  Tick-borne encephalitis virus in ticks detached from humans and follow-up of serological and clinical response. , 2014, Ticks and tick-borne diseases.

[17]  A. Movila,et al.  Detection of tick‐borne pathogens in ticks from migratory birds in the Baltic region of Russia , 2013, Medical and veterinary entomology.

[18]  J. Oteo,et al.  Genetic characterization of Candidatus Rickettsia vini, a new rickettsia amplified in ticks from La Rioja, Spain. , 2012, Ticks and tick-borne diseases.

[19]  Å. Lundkvist,et al.  Migratory Birds, Ticks, and Crimean-Congo Hemorrhagic Fever Virus , 2012, Emerging infectious diseases.

[20]  J. Oteo,et al.  Role of Birds in Dispersal of Etiologic Agents of Tick-borne Zoonoses, Spain, 2009 , 2012, Emerging infectious diseases.

[21]  T. Jaenson,et al.  Prevalence of Rickettsia spp., Anaplasma phagocytophilum, and Coxiella burnetii in adult Ixodes ricinus ticks from 29 study areas in central and southern Sweden. , 2012, Ticks and tick-borne diseases.

[22]  D. Fish,et al.  Prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus and I. lividus ticks collected from wild birds in the Republic of Moldova , 2008 .

[23]  S. Bergström,et al.  Migratory Passerine Birds as Reservoirs of Lyme Borreliosis in Europe , 2006, Emerging infectious diseases.

[24]  J. Stenos,et al.  A highly sensitive and specific real-time PCR assay for the detection of spotted fever and typhus group Rickettsiae. , 2005, The American journal of tropical medicine and hygiene.

[25]  Z. Hubálek.  AN ANNOTATED CHECKLIST OF PATHOGENIC MICROORGANISMS ASSOCIATED WITH MIGRATORY BIRDS , 2004, Journal of wildlife diseases.

[26]  B. Olsen,et al.  Geographical Distribution, Host Associations, and Vector Roles of Ticks (Acari: Ixodidae, Argasidae) in Sweden , 1994, Journal of medical entomology.

[27]  M. Carl,et al.  Diagnosis of acute typhus infection using the polymerase chain reaction. , 1990, The Journal of infectious diseases.

[28]  C. Mead.  Colony fidelity and interchange in the Sand Martin , 1979 .

[29]  A. Estrada-Peña,et al.  Ticks of Europe and North Africa , 2017, Springer International Publishing.

[30]  M. Ogrzewalska,et al.  Ticks ( Acari : Ixodidae ) as ectoparasites of Brazilian wild birds and their association with rickettsial diseases , 2016 .

[31]  P. Saikku,et al.  Ixodes lividus (Acari) in sand martin colonies in Fennoscandia , 1977 .