The effects of jasmonic acid and ethylenediamine-di-o-hydroxyphenyl-acetic acid on floral induction and induction of turions in Spirodela polyrhiza (L.) Schleiden

We investigated the effects of jasmonic acid (JA) and ethylenediamine-di-o-hydroxyphenyl-acetic acid (EDDHA) on floral induction and induction of turions in the photoperiodically neutral clone Djelekovec of the species Spirodela polyrhiza in axenical cultures. JA (0.475-47.5 nmol.L-1) promoted floral induction under long-day (LD) and short day (SD) conditions, while it had no effect on turion induction. The inhibitory effect of JA on flowering and turion induction was observed at a JA concentration of 237.5 nmol.L-1 and 475 nmpl.L-1. Under the same conditions, flowering and induction of turions were enhanced by EDDHA (20.5 nmol..L-1(. The combination od EDDHA and JA had an additive effect on promotion of floral induction, which was promoted significantly in experiments with LD preculture. The results obtained by quantitative determination of endogenous JA levels in Spirodela polyrhiza at two growth stages support our previous findings that JA may regulate floral induction. The levels of endogenous JA decreased from 226±12 ng·g–1 fresh weight during the vegetative stage in LD to 38.1±3.5 ng·g–1 in the flowering plants. In SD the levels of endogenous JA decreased from 62±9ng·g–1 fresh weight during vegetative stage to 29.2±3.1 ng·g–1 in the flowering plants.

[1]  J. Ullmann,et al.  Methyl jasmonate inhibits growth and flowering inChenopodium rubrum , 1994, Biologia Plantarum.

[2]  G. Barendse,et al.  Role of hormones on flower bud formation in thin-layer expiants of tobacco , 1985, Biologia Plantarum.

[3]  I. Janžekovič,et al.  Possible role of jasmonic acid in the regulation of floral induction, evocation and floral differentiation in Lemna minor L. , 2006, Plant physiology and biochemistry : PPB.

[4]  S. Yoshida,et al.  Inhibitory Effects of Jasmonic Acid and its Analogues on Barley (Hordeum vulgare L.) Anther Extrusion , 2006, Plant Growth Regulation.

[5]  M. Veber,et al.  Determination of jasmonic acid in Lemna minor (L.) by liquid chromatography with fluorescence detection , 2005, Analytical and bioanalytical chemistry.

[6]  Joseph R. Ecker,et al.  Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation , 2005, Development.

[7]  M. Zielińska,et al.  Jasmonates Inhibit Flowering in Short-Day Plant Pharbitis nil , 2004, Plant Growth Regulation.

[8]  B. Krajnčič,et al.  Mechanisms of EDDHA effects on the promotion of floral induction in the long-day plant Lemna minor (L.). , 2003, Journal of plant physiology.

[9]  K. Appenroth.  No photoperiodoc control of the formation of turions in eight clones of Spirodela polyrhiza. , 2003, Journal of plant physiology.

[10]  K. Appenroth.  Co-action of temperature and phosphate in inducing turion formation in Spirodela polyrhiza (Great duckweed) , 2002 .

[11]  A. Vogrin,et al.  Promotion of flowering by Mn-EDDHA in the long-short-day plant Wolffia arrhiza (L.) Horkel ex Wimm , 1998 .

[12]  R. Creelman,et al.  BIOSYNTHESIS AND ACTION OF JASMONATES IN PLANTS. , 1997, Annual review of plant physiology and plant molecular biology.

[13]  B. Krajnčič,et al.  The Effect of Jasmonic Acid on Flowering in Spirodela polyrrhiza (L.) Schleiden , 1995 .

[14]  A. Fleming,et al.  The Physiological Role of Abscisic Acid in Eliciting Turion Morphogenesis , 1995, Plant physiology.

[15]  J. Nemec,et al.  Promotion of flowering by Mn-EDDHA in the photoperiodically neutral plant Spirodela polyrrhiza (L.) Schleiden , 1995 .

[16]  K. Chaloupková,et al.  The Abscisic Acid Induction of a Novel Peroxidase Is Antagonized by Cytokinin in Spirodela polyrrhiza L , 1994, Plant physiology.

[17]  B. Krajnčič.  Synergistic effect of GA3 and EDDHA on the promotion of flowering in the long-short-day plants Wolffia arrhiza (L.) Wimm , 1994 .

[18]  B. Krajnčič,et al.  Synergistic Effect of GA3 and EDDHA on the Promotion of Flowering in the Photoperiodically Neutral Plant Spirodela polyrrhiza (L.) Schleiden , 1991 .

[19]  K. Appenroth,et al.  Phytochrome Control of Turion Formation in Spirodela polyrhiza L. Schleiden , 1990 .

[20]  L. Müller,et al.  Induction of flowering in Lemna gibba G3 under short-day conditions , 1977 .