Lactoferrin-derived resistance against plant pathogens in transgenic plants.

Lactoferrin (LF) is a ubiquitous cationic iron-binding milk glycoprotein that contributes to nutrition and exerts a broad-spectrum primary defense against bacteria, fungi, protozoa, and viruses in mammals. These qualities make lactoferrin protein and its antimicrobial motifs highly desirable candidates to be incorporated in plants to impart broad-based resistance against plant pathogens or to economically produce them in bulk quantities for pharmaceutical and nutritional purposes. This study introduced bovine LF (BLF) gene into tobacco ( Nicotiana tabacum var. Xanthi), Arabidopsis ( A. thaliana ) and wheat ( Triticum aestivum ) via Agrobacterium -mediated plant transformation. Transgenic plants or detached leaves exhibited high levels of resistance against the damping-off causing fungal pathogen Rhizoctonia solani and the head blight causing fungal pathogen Fusarium graminearum . LF also imparted resistance to tomato plants against a bacterial pathogen, Ralstonia solanacearum . Similarly, other researchers demonstrated expression of LF and LF-mediated high-quality resistance to several other aggressive fungal and bacterial plant pathogens in transgenic plants and against viral pathogens by foliar applications of LF or its derivatives. Taken together, these studies demonstrated the effectiveness of LF for improving crop quality and its biopharming potentials for pharmaceautical and nutritional applications.

[1]  A. Broz,et al.  Plant-Based Protein Biomanufacturing , 2013 .

[2]  Sujata Sharma,et al.  Antimicrobial Lactoferrin Peptides: The Hidden Players in the Protective Function of a Multifunctional Protein , 2013, International journal of peptides.

[3]  A. Atanassov,et al.  Expression of recombinant human lactoferrin in transgenic alfalfa plants , 2013, Biologia Plantarum.

[4]  Hongyan Wang,et al.  Inhibitory effect of esterified lactoferin and lactoferrin against tobacco mosaic virus (TMV) in tobacco seedlings. , 2013, Pesticide biochemistry and physiology.

[5]  J. Ueda,et al.  Transgenic tobacco plants expressing antimicrobial peptide bovine lactoferricin show enhanced resistance to phytopathogens , 2012 .

[6]  H. Vogel,et al.  Structural and biophysical characterization of an antimicrobial peptide chimera comprised of lactoferricin and lactoferrampin. , 2012, Biochimica et biophysica acta.

[7]  Y. Takayama.  Lactoferrin and its Role in Wound Healing , 2011, Springer Netherlands.

[8]  Jigang Han,et al.  Transgenic expression of lactoferrin imparts enhanced resistance to head blight of wheat caused by Fusarium graminearum , 2012, BMC Plant Biology.

[9]  G. Troup,et al.  Mode of action of milk and whey in the control of grapevine powdery mildew , 2006, Australasian Plant Pathology.

[10]  Ian McFarlane,et al.  The role of transgenic crops in sustainable development. , 2011, Plant biotechnology journal.

[11]  Miguel Calvo Rebollar,et al.  Recombinant human lactoferrin: a valuable protein for pharmaceutical products and functional foods. , 2010, Biotechnology advances.

[12]  O. Lund,et al.  Engineering pathogen resistance in crop plants: current trends and future prospects. , 2010, Annual review of phytopathology.

[13]  M. Andrés,et al.  Antimicrobial Mechanism of Action of Transferrins: Selective Inhibition of H+-ATPase , 2010, Antimicrobial Agents and Chemotherapy.

[14]  M. Sitohy,et al.  Inhibition of Tomato Yellow Leaf Curl Virus (TYLCV) using whey proteins , 2010, Virology Journal.

[15]  Xiang-ming Xu,et al.  Community ecology of fungal pathogens causing wheat head blight. , 2009, Annual review of phytopathology.

[16]  D. Walters.  Disease control in crops , 2009 .

[17]  N. Foroud,et al.  Trichothecenes in Cereal Grains , 2009, International journal of molecular sciences.

[18]  Scott M. Meyer,et al.  Integrated strategies for Fusarium head blight management in the United States , 2008 .

[19]  A. Atanassov,et al.  Production of recombinant human lactoferrin from transgenic plants , 2008, Biologia Plantarum.

[20]  Santosh Misra,et al.  Identification and rational design of novel antimicrobial peptides for plant protection. , 2008, Annual review of phytopathology.

[21]  W. Bettiol,et al.  Effectiveness of whey against zucchini squash and cucumber powdery mildew , 2008 .

[22]  E. Nester.  Agrobacterium: The Natural Genetic Engineer 100 Years Later , 2008 .

[23]  E. Kalra.  Nutraceutical-definition and introduction , 2008, AAPS PharmSci.

[24]  Y. Muhovski,et al.  Production of Recombinant Human Lactoferin in Transgenic Barley , 2007 .

[25]  J. Woo,et al.  Production of human lactoferrin in transgenic cell suspension cultures of sweet potato , 2006, Biologia Plantarum.

[26]  P. Valenti,et al.  Lactoferrin , 2005, Cellular and Molecular Life Sciences.

[27]  O. Conneely,et al.  Lactoferrin , 2005, Cellular and Molecular Life Sciences.

[28]  E. Inoue,et al.  Production and Characterization of Recombinant Human Lactoferrin in Transgenic Javanica Rice , 2005 .

[29]  T. Omura,et al.  Constitutive expression of human lactoferrin and its N-lobe in rice plants to confer disease resistance. , 2005, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[30]  C. Boucher,et al.  Lessons learned from the genome analysis of ralstonia solanacearum. , 2004, Annual review of phytopathology.

[31]  M. Malnoy,et al.  Expression of bovine lactoferrin cDNA confers resistance to Erwinia amylovora in transgenic pear , 2003, Molecular Breeding.

[32]  C. Flahaut,et al.  N-glycosylation potential of maize: The human lactoferrin used as a model , 2001, Glycoconjugate Journal.

[33]  W. Langridge,et al.  Expression of full-length bioactive antimicrobial human lactoferrin in potato plants , 2000, Transgenic Research.

[34]  R. W. Evans,et al.  Lactoferrin--a multifunctional protein with antimicrobial properties. , 2003, Molecular immunology.

[35]  S. Nandi,et al.  Expression of human lactoferrin in transgenic rice grains for the application in infant formula , 2002 .

[36]  E. Pauwels,et al.  Large scale production of recombinant human lactoferrin in the milk of transgenic cows , 2002, Nature Biotechnology.

[37]  T. Clemente,et al.  Partial Resistance to Bacterial Wilt in Transgenic Tomato Plants Expressing Antibacterial Lactoferrin Gene , 2002 .

[38]  R. Cameron,et al.  Age-Related Resistance in Arabidopsis Is a Developmentally Regulated Defense Response to Pseudomonas syringae Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010481. , 2002, The Plant Cell Online.

[39]  J. Lewis,et al.  Biocontrol of damping-off of greenhouse-grown crops caused by Rhizoctonia solani with a formulation of Trichoderma spp. , 2001 .

[40]  A. Mitra,et al.  Expression of Human Lactoferrin cDNA Confers Resistance to Ralstonia solanacearum in Transgenic Tobacco Plants. , 1998, Phytopathology.

[41]  V. Salmon,et al.  Production of human lactoferrin in transgenic tobacco plants. , 1998, Protein expression and purification.

[42]  D. Benson,et al.  Ornamental Diseases Incited by Rhizoctonia Spp. , 1996 .

[43]  B. Sneh,et al.  Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control , 1996, Springer Netherlands.

[44]  J. Lewis,et al.  Formulation and Delivery of Biocontrol Agents for Use Against Soilborne Plant Pathogens , 1995 .

[45]  O. Conneely,et al.  A System for Production of Commercial Quantities of Human Lactoferrin: A Broad Spectrum Natural Antibiotic , 1995, Bio/Technology.

[46]  G. Lomonossoff.  Pathogen-derived resistance to plant viruses. , 1995, Annual review of phytopathology.

[47]  A. Mitra,et al.  Expression of a Human Lactoferrin cDNA in Tobacco Cells Produces Antibacterial Protein(s) , 1994, Plant physiology.

[48]  P. Cotterill.  Evaluation of in-furrow fungicide treatments to control rhizoctonia root rot of wheat , 1991 .

[49]  G. Papavizas,et al.  Biocontrol of plant diseases: the approach for tomorrow , 1991 .

[50]  N. Anderson.  THE GENETICS AND PATHOLOGY OF RHIZOCTONIA SOLANI , 1982 .