NO-cGMP signaling molecules in the rat epithelial rests of Malassez.

The epithelial rests of Malassez (ERM) are derived from the disintegrating epithelial root sheath of Hertwig that guides root formation during tooth development. Low concentrations of nitric oxide (NO) produced by NO-synthase I (NOS I) and NOS III activate intracellular soluble guanylate cyclase (sGC) to produce intracellular cyclic guanosine 3':5'-monophosphate (cGMP), which triggers rapid cellular responses such as cell proliferation, cell differentiation, and apoptosis under physiological conditions. The presence of NOS I-III, sGC (alpha2- and beta1-subunits) and cGMP in the ERM was investigated by immunohistochemistry. Rat molars with periodontium were perfusion and postfixed, decalcified, frozen-sectioned, and sections were immunostained. NOS I, NOS III, sGC (alpha2- and beta1-subunits) and cGMP were localized with different densities in the ERM. The presence of NOS II in the ERM varied. The localization of NOS I, NOS III, sGC and cGMP in the ERM indicates an involvement of NO and/or NO-cGMP signal pathway molecules in homeostasis of a variety of physiological processes in the ERM. These could include regulation of cell proliferation, cell differentiation and apoptosis.

[1]  P. Wesselink,et al.  The prevalence and distribution of rests of Malassez in the mouse molar and their possible role in repair and maintenance of the periodontal ligament. , 1993, Archives of oral biology.

[2]  Y. Yoshimura,et al.  Cyclic tension force activates nitric oxide production in cultured human periodontal ligament cells. , 2000, Journal of periodontology.

[3]  R. Robitaille,et al.  Localization and characterization of nitric oxide synthase at the frog neuromuscular junction , 1998, Journal of neurocytology.

[4]  P. Cerri,et al.  Apoptosis in the early developing periodontium of rat molars , 2000, The Anatomical record.

[5]  H. Ogihara,et al.  Inducible nitric oxide synthase activity by interferon-gamma-producing cells in human radicular cysts. , 1999, International endodontic journal.

[6]  R. Burry,et al.  Specificity Controls for Immunocytochemical Methods , 2000, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[7]  D. van Steenberghe,et al.  Periodontal neural endings intimately relate to epithelial rests of Malassez in humans. A light and electron microscope study. , 1993, Journal of anatomy.

[8]  J. Kempfert,et al.  Developmental changes of nitric oxide-sensitive guanylyl cyclase expression in pulmonary arteries. , 2001, Biochemical and biophysical research communications.

[9]  A. Zeiher,et al.  Nitric oxide and apoptosis: another paradigm for the double-edged role of nitric oxide. , 1997, Nitric oxide : biology and chemistry.

[10]  D. Kinane,et al.  Inducible nitric oxide synthase expression in periodontitis. , 2000, Journal of periodontal research.

[11]  G. Enikolopov,et al.  Nitric Oxide Regulates Cell Proliferation during Drosophila Development , 1996, Cell.

[12]  Grigori Enikolopov,et al.  Nitric oxide triggers a switch to growth arrest during differentiation of neuronal cells , 1995, Nature.

[13]  C. Andressen,et al.  Nitric oxide synthase expression and role during cardiomyogenesis. , 1999, Cardiovascular research.

[14]  Carl Nathan,et al.  Nitric oxide synthases: Roles, tolls, and controls , 1994, Cell.

[15]  H. Kumamoto,et al.  Expression of inducible nitric oxide synthase and heat shock proteins in periapical inflammatory lesions. , 2002, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology.

[16]  A. Newby,et al.  Nitric oxide and the proliferation of vascular smooth muscle cells. , 1999, Cardiovascular research.

[17]  J. D. Spouge.  A new look at the rests of Malassez. A review of their embryological origin, anatomy, and possible role in periodontal health and disease. , 1980, Journal of periodontology.

[18]  Y. Hamamoto,et al.  Ultrastructure of epithelial rests of Malassez in human periodontal ligament. , 1989, Archives of oral biology.

[19]  A. R. Cate.  The epithelial cell rests of Malassez and the genesis of the dental cyst. , 1972 .

[20]  M. Marletta,et al.  Guanylate cyclase and the .NO/cGMP signaling pathway. , 1999, Biochimica et biophysica acta.

[21]  H. Kendall,et al.  Nitric oxide synthase type-II is synthesized by human gingival tissue and cultured human gingival fibroblasts. , 2000, Journal of periodontal research.

[22]  T. Billiar,et al.  Nitric oxide as a bifunctional regulator of apoptosis. , 1999, Circulation research.

[23]  Y. Hamamoto,et al.  Morphological changes of epithelial rests of Malassez in rat molars induced by local administration of N-methylnitrosourea. , 1998, Archives of oral biology.

[24]  J. Kempfert,et al.  Expression and tissue localization of soluble guanylyl cyclase in the human placenta using novel antibodies directed against the alpha(2) subunit. , 2001, The Journal of clinical endocrinology and metabolism.

[25]  C. Harteneck,et al.  A Variant of the α2 Subunit of Soluble Guanylyl Cyclase Contains an Insert Homologous to a Region within Adenylyl Cyclases and Functions as a Dominant Negative Protein (*) , 1995, The Journal of Biological Chemistry.

[26]  Yan Wang,et al.  Epithelial Rests of Malassez Express Immunoreactivity of TrkA and Its Distribution Is Regulated by Sensory Nerve Innervation , 2000, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[27]  W. Timens,et al.  Specificity of Antibodies to Nitric Oxide Synthase Isoforms in Human, Guinea Pig, Rat, and Mouse Tissues , 1998, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[28]  James B. Mitchell,et al.  The multifaceted roles of nitric oxide in cancer. , 1998, Carcinogenesis.