Bovine Colostrum Whey Protein Hydrolysate Inhibits Cell DNA Damage and LDL Oxidation In Vitro

Whey protein isolated from bovine colostrums collected on the second day postpartum was two-stage hydrolyzed by alcalase and flavourzyme. The whey hydrolysates were finally fractionated by ultrafiltration (UF) with a 10 kDa molecular weight (MW) cutoff membrane and subsequently used to evaluate the effect of whey protein hydrolysis on inhibition of DNA oxidative damage and low-density lipoprotein (LDL) oxidation in vitro. Results showed that whey hydrolysis exhibited not only higher inhibitory activities of oxidative damage of deoxyribose but also an inhibitory effect on the breakdown of supercoiled DNA into open circular DNA and linear DNA. The quantities of 8-hydroxy-2′-deoxyguanosine (8-OH-2′-dG) formed with the addition of whey hydrolysate protein, the hydrolysate fraction of MW >10 kDa, and the hydrolysate fraction of MW <10 kDa were 0.25, 0.06, and 0.09 μg/mL, respectively. The lag time of conjugated diene formation of the control sample, which was only combined with cupric ions and LDL, was 90 min. The samples added with the hydrolysate fractions exhibited higher inhibitory activity on LDL oxidation. The whey hydrolysate fractions extended the lag time of conjugated diene formation to 270 min. The lag time of the whey hydrolysate fractions was 3 times that of the control.

[1]  Chi-Yue Chang,et al.  The Inhibition Effect of Cell DNA Oxidative Damage and LDL Oxidation by Bovine Colostrums , 2016, Molecules.

[2]  Jakub Treml,et al.  Flavonoids as Potent Scavengers of Hydroxyl Radicals. , 2016, Comprehensive reviews in food science and food safety.

[3]  Xueliang Wang,et al.  Study on DNA damage induced by the reactive oxygen species generated in situ based on the multi-walled carbon nanotubes and hemoglobin , 2016 .

[4]  A. Brandelli,et al.  Whey as a source of peptides with remarkable biological activities , 2015 .

[5]  Chi-Yue Chang,et al.  GROWTH INHIBITION AND DIFFERENTIATING EFFECTS OF PROTEIN HYDROLYSATES FROM BOVINE COLOSTRUMS ON HUMAN LEUKEMIC U937 CELLS , 2013 .

[6]  S. C. B. Oliveira,et al.  In situ DNA oxidative damage by electrochemically generated hydroxyl free radicals on a boron-doped diamond electrode. , 2012, Langmuir : the ACS journal of surfaces and colloids.

[7]  D. Steinberg.  The LDL modification hypothesis of atherogenesis: an update Published, JLR Papers in Press, November 15, 2009. , 2009, Journal of Lipid Research.

[8]  K. Hagiwara,et al.  Bovine colostral CD8-positive cells are potent IFN-gamma-producing cells. , 2008, Veterinary immunology and immunopathology.

[9]  A. Yalçın,et al.  Antioxidant activity of whey protein fractions isolated by gel exclusion chromatography and protease treatment. , 2008, Talanta.

[10]  H. Kanegane,et al.  Role of transforming growth factor-beta in breast milk for initiation of IgA production in newborn infants. , 2004, Early human development.

[11]  H. Neil,et al.  The relation between dietary flavonol intake and coronary heart disease mortality: a meta-analysis of prospective cohort studies , 2003, European Journal of Clinical Nutrition.

[12]  S. Heymsfield,et al.  Effects of whey protein and resistance exercise on body cell mass, muscle strength, and quality of life in women with HIV , 2001, AIDS.

[13]  G. Bounous.  Whey protein concentrate (WPC) and glutathione modulation in cancer treatment. , 2000, Anticancer research.

[14]  R. Playford,et al.  Colostrum and milk-derived peptide growth factors for the treatment of gastrointestinal disorders. , 2000, The American journal of clinical nutrition.

[15]  G. Yen,et al.  Antioxidant actions of du-zhong (Eucommia ulmoides Oliv.) toward oxidative damage in biomolecules. , 2000, Life sciences.

[16]  L. Lands,et al.  Effect of supplementation with a cysteine donor on muscular performance. , 1999, Journal of applied physiology.

[17]  M. Shih,et al.  Ascorbic acid inhibits lipid peroxidation but enhances DNA damage in rat liver nuclei incubated with iron ions. , 1997, Free radical research.

[18]  G. Yen,et al.  Antioxidant and Pro-Oxidant Effects of Various Tea Extracts , 1997 .

[19]  T. Umemura,et al.  Inhibitory effects of vitamin E and ellagic acid on 8-hydroxydeoxyguanosine formation in liver nuclear DNA of rats treated with 2-nitropropane. , 1995, Cancer letters.

[20]  J. Kleinjans,et al.  Induction of oxidative DNA damage and early lesions in rat gastro-intestinal epithelium in relation to prostaglandin H synthase-mediated metabolism of butylated hydroxyanisole. , 1995, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[21]  G. Erdem,et al.  Free radical mediated interaction of ascorbic acid and ascorbate/Cu(II) with viral and plasmid DNAs , 1994, Journal of Biosciences.

[22]  B. Halliwell,et al.  Evaluation of the antioxidant and prooxidant actions of gallic acid and its derivatives , 1993 .

[23]  O. Aruoma,et al.  Protection by albumin against the pro-oxidant actions of phenolic dietary components. , 1992, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[24]  E. Stadtman,et al.  Ascorbic acid and oxidative inactivation of proteins. , 1991, The American journal of clinical nutrition.

[25]  L. Povirk,et al.  Genotoxicity of bleomycin. , 1991, Mutation research.

[26]  M. Díaz-Castañeda,et al.  Production of Fish Protein Hydrolysates with Bacterial Proteases; Yield and Nutritional Value , 1991 .

[27]  A. Grollman,et al.  Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG , 1991, Nature.

[28]  J. Huet,et al.  Potentiation of cell killing by inhibitors of poly(adenosine diphosphate-ribose) synthesis in bleomycin-treated Chinese hamster ovary cells. , 1985, Cancer research.

[29]  S. Nishimura,et al.  Hydroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agents. , 1984, Nucleic acids research.

[30]  K. Kondo,et al.  Green tea catechins prevent low-density lipoprotein oxidation via their accumulation in low-density lipoprotein particles in humans. , 2016, Nutrition research.

[31]  H. Paik,et al.  Whey protein inhibits iron overload-induced oxidative stress in rats. , 2013, Journal of nutritional science and vitaminology.

[32]  I. Hertz-Picciotto,et al.  Comparison of organochlorine compound concentrations in colostrum and mature milk. , 2007, Chemosphere.

[33]  S. Omaye,et al.  β-Carotene: interactions with α-tocopherol and ascorbic acid in microsomal lipid peroxidation , 2001 .

[34]  A. Watanabe,et al.  Nutritional therapy of chronic hepatitis by whey protein (non-heated). , 2000, Journal of medicine.

[35]  J. Abian,et al.  Complexes of iron with phenolic compounds from soybean nodules and other legume tissues: prooxidant and antioxidant properties. , 1997, Free radical biology & medicine.

[36]  B. Ames,et al.  Assays for 8-hydroxy-2'-deoxyguanosine: a biomarker of in vivo oxidative DNA damage. , 1991, Free radical biology & medicine.

[37]  K. Ueda,et al.  The effects of metal ions on the DNA damage induced by hydrogen peroxide. , 1990, Agricultural and biological chemistry.

[38]  L. G. Ensminger.  The Association of Official Analytical Chemists , 1976 .