Ultrasensitive photoelectrochemical immunoassay of indole-3-acetic acid based on the MPA modified CdS/RGO nanocomposites decorated ITO electrode.

A novel ultrasensitive photoelectrochemical immunosensor was fabricated based on 3-mercaptopropionic acid stabilized CdS/reduced graphene oxide (MPA-CdS/RGO) nanocomposites for indole-3-acetic acid (IAA) detection. The MPA-CdS/RGO nanocomposites were synthesized by in situ solvothermal growth of triangulated pyramidal CdS nanoparticles on the RGO sheet. 3-Mercaptopropionic acid (MPA) was employed as the modifier and bridge to immobilize the antibody. The nanocomposites were characterized by X-ray diffraction, transmission electron microscopy, scanning electron microscopy and UV/vis spectra. The results showed that the MPA-CdS/RGO nanocomposites revealed enhanced photocurrent response due to excellent electron transport properties of RGO and the improved assembly of CdS nanoparticles onto RGO sheet with the introduction of MPA. Based on the dependence of the photocurrent decline on the concentration of IAA, the proposed photoelectrochemical immunosensor for IAA depicted a linear range from 0.1 to 1000 ng/mL with a lower detection limit (0.05 ng/mL). The high sensitivity, reproducibility and specificity of the method permitted the method suitable to be used in real samples.

[1]  A. Hagfeldt,et al.  Highly efficient CdS quantum dot-sensitized solar cells based on a modified polysulfide electrolyte. , 2011, Journal of the American Chemical Society.

[2]  Yang Li,et al.  Ultrasensitive determination of cysteine based on the photocurrent of nafion-functionalized CdS-MV quantum dots on an ITO electrode. , 2011, Small.

[3]  Michael Holzinger,et al.  Electrogenerated trisbipyridyl Ru(II)-/nitrilotriacetic-polypyrene copolymer for the easy fabrication of label-free photoelectrochemical immunosensor and aptasensor: application to the determination of thrombin and anti-cholera toxin antibody. , 2013, Biosensors & bioelectronics.

[4]  Gui Yin,et al.  Phthalocyanine-sensitized graphene-CdS nanocomposites: an enhanced photoelectrochemical immunosensing platform. , 2013, Chemistry.

[5]  Xiaoru Zhang,et al.  A new signal-on photoelectrochemical biosensor based on a graphene/quantum-dot nanocomposite amplified by the dual-quenched effect of bipyridinium relay and AuNPs. , 2012, Chemistry.

[6]  M. Jaroniec,et al.  Graphene-based semiconductor photocatalysts. , 2012, Chemical Society Reviews.

[7]  Yanli Chang,et al.  A Facile One‐step Method to Produce Graphene–CdS Quantum Dot Nanocomposites as Promising Optoelectronic Materials , 2010, Advanced materials.

[8]  Meicheng Yang,et al.  A photoelectrochemical immunosensor based on Au-doped TiO2 nanotube arrays for the detection of α-synuclein. , 2010, Chemistry.

[9]  Wei-Wei Zhao,et al.  Highly sensitive photoelectrochemical immunoassay with enhanced amplification using horseradish peroxidase induced biocatalytic precipitation on a CdS quantum dots multilayer electrode. , 2012, Analytical chemistry.

[10]  D. Weijers,et al.  Auxin control of embryo patterning. , 2009, Cold Spring Harbor perspectives in biology.

[11]  Na Li,et al.  Quantum dot-based near-infrared electrochemiluminescent immunosensor with gold nanoparticle-graphene nanosheet hybrids and silica nanospheres double-assisted signal amplification. , 2012, Analytical chemistry.

[12]  G. Wallace,et al.  Processable aqueous dispersions of graphene nanosheets. , 2008, Nature nanotechnology.

[13]  Itamar Willner,et al.  Photoelectrochemical Biosensors Without External Irradiation: Probing Enzyme Activities and DNA Sensing Using Hemin/G-Quadruplex-Stimulated Chemiluminescence Resonance Energy Transfer (CRET) Generation of Photocurrents , 2012 .

[14]  C. Vaz,et al.  Use of a graphite–polyurethane composite electrode for electroanalytical determination of indole-3-acetic acid in soil samples , 2007 .

[15]  Guohe Huang,et al.  A renewable amperometric immunosensor for phytohormone β-indole acetic acid assay , 2003 .

[16]  Hong Wang,et al.  Simultaneous determination of phytohormones containing carboxyl in crude extracts of fruit samples based on chemical derivatization by capillary electrophoresis with laser-induced fluorescence detection. , 2011, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[17]  Peng Wang,et al.  Photoelectrochemistry of free-base-porphyrin-functionalized zinc oxide nanoparticles and their applications in biosensing. , 2011, Chemistry.

[18]  K. Novoselov,et al.  Giant intrinsic carrier mobilities in graphene and its bilayer. , 2007, Physical review letters.

[19]  Yunlei Zhou,et al.  An ultrasensitive electrochemical immunosensor platform with double signal amplification for indole-3-acetic acid determinations in plant seeds. , 2013, The Analyst.

[20]  Xiaoru Zhang,et al.  Aptamer based photoelectrochemical cytosensor with layer-by-layer assembly of CdSe semiconductor nanoparticles as photoelectrochemically active species. , 2011, Biosensors & bioelectronics.

[21]  Qin Xu,et al.  Poly(3-hexylthiophene)/TiO2 nanoparticle-functionalized electrodes for visible light and low potential photoelectrochemical sensing of organophosphorus pesticide chlopyrifos. , 2011, Analytical chemistry.

[22]  Tiangang Luan,et al.  Simultaneous Determination of Phytohormones in Plant Extracts using SPME and HPLC , 2007 .

[23]  Bin Hu,et al.  Simultaneous determination of several phytohormones in natural coconut juice by hollow fiber-based liquid-liquid-liquid microextraction-high performance liquid chromatography. , 2009, Journal of chromatography. A.

[24]  Bing Sun,et al.  A novel photoelectrochemical sensor based on PPIX-functionalized WO3-rGO nanohybrid-decorated ITO electrode for detecting cysteine. , 2013, Biosensors & bioelectronics.

[25]  Xuemin Wang,et al.  Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography–mass spectrometry , 2010, Nature Protocols.

[26]  Jun-Jie Zhu,et al.  Dual-signal amplification strategy for ultrasensitive photoelectrochemical immunosensing of α-fetoprotein. , 2012, Analytical chemistry.

[27]  S. Stankovich,et al.  Graphene-based composite materials , 2006, Nature.

[28]  C. Perrot-Rechenmann,et al.  Recent progress in auxin biology. , 2010, Comptes rendus biologies.

[29]  M. Buiatti,et al.  Comparative determination of some phytohormones in wild-type and genetically modified plants by gas chromatography-mass spectrometry and high-performance liquid chromatography-tandem mass spectrometry. , 2010, Analytical biochemistry.

[30]  Z. Zhang,et al.  Determination of indole-3-acetic acid and indole-3-butyric acid in mung bean sprouts using high performance liquid chromatography with immobilized Ru(bpy)3(2+)-KMnO4 chemiluminescence detection. , 2009, Talanta.

[31]  Serge Cosnier,et al.  Photoelectrochemical immunosensor for label-free detection and quantification of anti-cholera toxin antibody. , 2006, Journal of the American Chemical Society.

[32]  Qingming Shen,et al.  Graphene-CdS nanocomposites: facile one-step synthesis and enhanced photoelectrochemical cytosensing. , 2012, Chemistry.

[33]  I. Willner,et al.  Efficient generation of photocurrents by using CdS/carbon nanotube assemblies on electrodes. , 2004, Angewandte Chemie.

[34]  Suqin Han.  Chemiluminescence determination of indole derivatives in human body fluids and soil by flow injection analysis using potassium permanganate , 2010 .

[35]  Qingming Shen,et al.  Fabrication of glutathione photoelectrochemical biosensor using graphene-CdS nanocomposites. , 2012, The Analyst.

[36]  Tsuyoshi Takata,et al.  Self-Templated Synthesis of Nanoporous CdS Nanostructures for Highly Efficient Photocatalytic Hydrogen Production under Visible Light , 2008 .

[37]  Yadong Li,et al.  Direct synthesis of water-soluble ultrathin CdS nanorods and reversible tuning of the solubility by alkalinity. , 2010, Journal of the American Chemical Society.

[38]  Shengshui Hu,et al.  A disposable electrochemical sensor for the determination of indole-3-acetic acid based on poly(safranine T)-reduced graphene oxide nanocomposite. , 2011, Talanta.