Ovulation Induction Effect of Sevenband Grouper, Epinephelus septemfasciatus by Treating Hormones

Abstract This study aims to investigate how ovulation induction of sevenband grouper, Epinephelus septemfasciatus varies when treated with different hormonal substances such as ovaprim, pimozide, LHRHa, and HCG. As for LHRHa, we injected it in different concentrations: 50 ㎍/kg, 100 ㎍/kg, 150 ㎍/kg, and 200 ㎍/kg. All hormonal substances including LHRHa were injected into back muscles. As a result, ovulation occurred in all sample groups. The sample group injected with HCG released the largest amount of eggs, and the dimeter of eggs and oil globules were also significantly bigger than those of others. Fertilization rate, embryonic survival rate, and hatching rate were the highest in a LHRHa (100 ㎍/kg) +pimozide (1,000 ㎍/kg) group and buoyant rate in a LHRHa group. Ovulation rate was 100% when LHRHa was injected in different concentrations. The largest amount of eggs was released in 200 ㎍/kg. The diameter of eggs and oil globules were the biggest in 200 ㎍/kg and the smallest in 50 ㎍/kg. Fertilization rate showed no significant difference through all different levels of concentration: 94.6-95.3%. Buoyant rate was the highest in 100 ㎍/kg and embryonic survival rate and hatching rate in 150 ㎍/kg. Based on these experimental results, we concluded that LHRHa in 100 ㎍/kg is the most efficient for the ovulation induction of sevenband grouper.Key words : Sevenband grouper, Ovulation induction, Ovaprim, LHRHa, Pimozide, HCGCorresponding author : 061-690-8977, hwgang@korea.kr 2015 (RP-2015-AQ-034).

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