Cantilever-type electrode array-based high-throughput microparticle sorting platform driven by gravitation and negative dielectrophoretic force

In this paper, we describe a cantilever-type electrode (CE) array-based high-throughput sorting platform, which is a tool used to separate microparticles using gravitation and negative dielectrophoretic (n-DEP) force. This platform consists of meso-size channels and a CE array, which is designed to separate a large number of target particles by differences in their dielectric material properties (DMP) and the weight of the particles. We employ a two-step separation process, with sedimentation as the first step and n-DEP as the second step. In order to differentiate the weight and the DMP of each particle, we employ the sedimentation phenomena in a vertical channel and the CE-based n-DEP in an inclined channel. By using three kinds of polystyrene beads with diameters of 10, 25 and 50 µm, the optimal population (107 beads ml−1) of particles and the appropriate length (25 mm) of the vertical channel for high performance were determined experimentally. Conclusively, by combining sedimentation and n-DEP schemes, we achieve 74.5, 94.7 and 100% separation efficiency for sorting microparticles with a diameter of 10, 25 and 50 µm, respectively.

[1]  Conrad D. James,et al.  Continuous-mode dielectrophoretic gating for highly efficient separation of analytes in surface micromachined microfluidic devices , 2008 .

[2]  H. Stone,et al.  Cellular-scale hydrodynamics , 2008, Biomedical materials.

[3]  J. Thomson,et al.  Dielectrophoretic separation of platelets from diluted whole blood in microfluidic channels , 2008, Electrophoresis.

[4]  Karan V. I. S. Kaler,et al.  Dielectrophoretic fluidic cell fractionation system , 2004 .

[5]  H. John Crabtree,et al.  Continuous dielectrophoretic cell separation microfluidic device. , 2007, Lab on a chip.

[6]  J. Yeow,et al.  Enhancing dielectrophoresis effect through novel electrode geometry , 2007, Biomedical microdevices.

[7]  S. Takayama,et al.  Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification. , 2007, Analytical chemistry.

[8]  Byungkyu Kim,et al.  Effect of a blind spot in a dielectrophoretic field on the separation of human breast cancer cells (MCF 7) , 2009 .

[9]  Elisabeth Smela,et al.  Multiple frequency dielectrophoresis , 2007, Electrophoresis.

[10]  Minoru Seki,et al.  Continuous separation of particles using a microfluidic device equipped with flow rate control valves. , 2006, Journal of chromatography. A.

[11]  Young-Ho Cho,et al.  A continuous cell separation chip using hydrodynamic dielectrophoresis (DEP) process , 2005 .

[12]  A. B. Frazier,et al.  Lateral-driven continuous dielectrophoretic microseparators for blood cells suspended in a highly conductive medium. , 2008, Lab on a chip.

[13]  Sungyoung Choi,et al.  Microfluidic system for dielectrophoretic separation based on a trapezoidal electrode array. , 2005, Lab on a chip.

[14]  Ciprian Iliescu,et al.  Dielectrophoretic separation of biological samples in a 3D filtering chip , 2007 .

[15]  Andreas Manz,et al.  On-chip free-flow magnetophoresis: continuous flow separation of magnetic particles and agglomerates. , 2004, Analytical chemistry.

[16]  Milica Radisic,et al.  Micro- and nanotechnology in cell separation , 2006, International journal of nanomedicine.

[17]  Y. Huang,et al.  Introducing dielectrophoresis as a new force field for field-flow fractionation. , 1997, Biophysical journal.

[18]  Unyoung Kim,et al.  Multitarget magnetic activated cell sorter , 2008, Proceedings of the National Academy of Sciences.

[19]  Y. Huang,et al.  Cell separation by dielectrophoretic field-flow-fractionation. , 2000, Analytical chemistry.

[20]  S. Takayama,et al.  Microfluidics for flow cytometric analysis of cells and particles , 2005, Physiological measurement.

[21]  P. Renaud,et al.  Focusing and Continuous Separation of Cells in a Microfluidic Device using Lateral Dielectrophoresis , 2007, TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference.

[22]  D. Beebe,et al.  Microenvironment design considerations for cellular scale studies. , 2004, Lab on a chip.

[23]  Byungkyu Kim,et al.  Novel platform for minimizing cell loss on separation process: Droplet-based magnetically activated cell separator. , 2007, The Review of scientific instruments.

[24]  Michael P Hughes,et al.  Strategies for dielectrophoretic separation in laboratory‐on‐a‐chip systems , 2002, Electrophoresis.

[25]  M. Yamada,et al.  Hydrodynamic filtration for on-chip particle concentration and classification utilizing microfluidics. , 2005, Lab on a chip.

[26]  David W. Inglis,et al.  Efficient microfluidic particle separation arrays , 2009 .

[27]  P. Gascoyne,et al.  Particle separation by dielectrophoresis , 2002, Electrophoresis.

[28]  Jung-Yeul Jung,et al.  Separation of microparticles and biological cells inside an evaporating droplet using dielectrophoresis. , 2007, Analytical chemistry.

[29]  Thomas Braschler,et al.  Focusing and Continuous Separation of Cells in a Microfluidic Device using Lateral Dielectrophoresis , 2007 .

[30]  S. Digumarthy,et al.  Isolation of rare circulating tumour cells in cancer patients by microchip technology , 2007, Nature.

[31]  J. Sturm,et al.  Continuous Particle Separation Through Deterministic Lateral Displacement , 2004, Science.

[32]  Do-Hyun Lee,et al.  Dielectrophoretic oocyte selection chip for in vitro fertilization , 2008, Biomedical microdevices.

[33]  Ming-Chih Ho,et al.  A planar interdigitated ring electrode array via dielectrophoresis for uniform patterning of cells. , 2008, Biosensors & bioelectronics.

[34]  H. Du,et al.  A dielectrophoretic barrier-based microsystem for separation of microparticles , 2007 .

[35]  Claus Duschl,et al.  Gravitation-driven stress-reduced cell handling , 2008, Analytical and bioanalytical chemistry.

[36]  Byungkyu Kim,et al.  Separation of malignant human breast cancer epithelial cells from healthy epithelial cells using an advanced dielectrophoresis-activated cell sorter (DACS) , 2009, Analytical and bioanalytical chemistry.

[37]  H. Morgan,et al.  The dielectrophoretic and travelling wave forces generated by interdigitated electrode arrays: analytical solution using Fourier series , 2001 .

[38]  Peter R C Gascoyne,et al.  Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation. , 2008, Lab on a chip.

[39]  Hywel Morgan,et al.  AC ELECTROKINETICS: COLLOIDS AND NANOPARTICLES. , 2002 .

[40]  Andreas Radbruch,et al.  Detection and isolation of rare cells. , 1995, Current opinion in immunology.

[41]  Paul H. Bessette,et al.  Marker-specific sorting of rare cells using dielectrophoresis. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[42]  P. Pittet,et al.  Fast prototyping using a dry film photoresist: microfabrication of soft-lithography masters for microfluidic structures , 2007 .

[43]  Chih-Ming Ho,et al.  Cell Separation by Non-Inertial Force Fields in Microfluidic Systems. , 2009, Mechanics research communications.

[44]  Minoru Seki,et al.  Continuous and size-dependent sorting of emulsion droplets using hydrodynamics in pinched microchannels. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[45]  Sungyoung Choi,et al.  Continuous hydrophoretic separation and sizing of microparticles using slanted obstacles in a microchannel. , 2007, Lab on a chip.

[46]  Unyoung Kim,et al.  Simultaneous sorting of multiple bacterial targets using integrated dielectrophoretic-magnetic activated cell sorter. , 2009, Lab on a chip.

[47]  Thomas Laurell,et al.  Chip integrated strategies for acoustic separation and manipulation of cells and particles. , 2007, Chemical Society reviews.