Evaluation of Commercially Prepared Transport Systems for Nonlethal Detection of Aeromonas salmonicida in Salmonid Fish

Abstract In vitro studies indicated that commercially prepared transport systems containing Amies, Stuart's, and Cary–Blair media worked equally well in sustaining the viability of the fish pathogen Aeromonas salmonicida, which causes furunculosis. The bacterium remained viable without significant increase or decrease in cell numbers for as long as 48 h of incubation at 18–20°C in Stuart's transport medium; consequently, obtaining mucus samples in such tubes were comparable to on-site detection of A. salmonicida by dilution plate counts on Coomassie Brilliant Blue agar. In three different assays of 100 samples of mucus from Atlantic salmon Salmo salar infected subclinically with A. salmonicida, dilution counts conducted on-site proved more reliable for detecting the pathogen than obtaining the samples in the transport system. In the on-site assays, dilution counts detected the pathogen in 34, 41, and 22 samples, whereas this was accomplished in only 15, 15, and 3 of the respective samples when the transpo...

[1]  R. Cipriano,et al.  Control of Furunculosis and Enteric Redmouth Disease in Sea‐Run Atlantic Salmon Broodstock in the Connecticut and Merrimack Rivers , 1998 .

[2]  Peter Smith,et al.  Covert Aeromonas salmonicida Infections , 1997 .

[3]  R. Cipriano,et al.  Monitoring for Early Detection of Aeromonas salmonicida to Enhance Antibiotic Therapy and Control Furunculosis in Atlantic Salmon , 1996 .

[4]  J. Teska,et al.  Use of non-lethal procedures to detect and monitor Aeromonas salmonicida in potentially endangered or threatened populations of migrating and post-spawning salmon , 1996 .

[5]  L. Ford.  Health management: Atlantic salmon restoration effort , 1996 .

[6]  L. Ford.  Detection of Aeromonas salmonicida from water using a filtration method , 1994 .

[7]  J. H. Schachte,et al.  Evaluation of mucus as a valid site to isolate Aeromonas salmonicida among asymptomatic populations of lake trout (Salvelinus namaycush) , 1994 .

[8]  Peter Smith,et al.  Detection of Aeromonas salmonicida in Atlantic salmon with asymptomatic furunculosis infections. , 1994 .

[9]  Peter Smith,et al.  Evidence for the transient nature of stress inducible asymptomatic Aeromonas salmonicida infections of Atlantic salmon. , 1993 .

[10]  J. Teska,et al.  Detection of Aeromonas salmonicida in the Mucus of Salmonid Fishes , 1992 .

[11]  K. Amos.  Procedures for the detection and identification of certain fish pathogens , 1985 .

[12]  A. Dubois-Darnaudpeys,et al.  Epidémiologie de la furonculose des salmonidés. - II - Etude expérimentale de divers facteurs microbiotiques de l'environnement. , 1977 .

[13]  J. Cornick,et al.  Habitat and Viability Studies on Aeromonas Salmonicida, Causative Agent of Furunculosis , 1969 .

[14]  Amies Cr.  A modified formula for the preparation of Stuart's Transport Medium. , 1967 .

[15]  M. Fusillo,et al.  SURVIVAL OF SHIGELLA AND SALMONELLA IN A NEW TRANSPORT MEDIUM. , 1965, Technical bulletin of the Registry of Medical Technologists. American Society of Clinical Pathologists. Registry of Medical Technologists.

[16]  E. Blair,et al.  NEW TRANSPORT MEDIUM FOR SHIPMENT OF CLINICAL SPECIMENS I , 1964, Journal of bacteriology.

[17]  R. Stuart,et al.  The problem of transport of specimens for culture of Gonococci. , 1954, Canadian journal of public health = Revue canadienne de sante publique.

[18]  R. Stuart,et al.  Centralized Gonococcus Culture for Dispersed Clinics , 1948 .