A protein detection technique by using surface plasmon resonance (SPR) with rolling circle amplification (RCA) and nanogold-modified tags.

Surface plasmon resonance (SPR) can detect molecules bound to a surface by subtle changes in the SPR angle. By immobilizing probes onto the surface and passing analyte solution through the surface, changes in SPR angle indicate the binding between analyte and probes. Detection of analyte from solution can be achieved easily. By using rolling circle amplification (RCA) and nanogold-modified tags, the signals of analyte binding are greatly amplified, and the sensitivity of this technique is significantly improved. Furthermore, this technique has potentials for ultra-sensitive detection and microarray analysis. In this paper, this detection technique is introduced and shown to have great amplification capability. Using 5 nm nanogold with 30 min of RCA development time, this proposed protein detection technique shows over 60 times amplification of the original signal.

[1]  R. Nelson,et al.  Surface plasmon resonance biomolecular interaction analysis mass spectrometry. 1. Chip-based analysis. , 1997, Analytical chemistry.

[2]  D G Myszka,et al.  BIACORE J: a new platform for routine biomolecular interaction analysis † , 2001, Journal of molecular recognition : JMR.

[3]  Reinhard Renneberg,et al.  Nanocrystal biolabels with releasable fluorophores for immunoassays. , 2004, Analytical chemistry.

[4]  W. März,et al.  Immuno-PCR with a commercially available avidin system. , 1993, Science.

[5]  C R Cantor,et al.  Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates. , 1992, Science.

[6]  R. Fisher,et al.  Universal immuno-PCR for ultra-sensitive target protein detection. , 1993, Nucleic acids research.

[7]  M. Natan,et al.  Colloidal Au-enhanced surface plasmon resonance immunosensing. , 1998, Analytical chemistry.

[8]  Lin He,et al.  Colloidal Au-Enhanced Surface Plasmon Resonance for Ultrasensitive Detection of DNA Hybridization , 2000 .

[9]  Matthias Seydack,et al.  Nanoparticle labels in immunosensing using optical detection methods. , 2005, Biosensors & bioelectronics.

[10]  R. Joerger,et al.  High sensitivity multianalyte immunoassay using covalent DNA-labeled antibodies and polymerase chain reaction. , 1995, Nucleic acids research.

[11]  Yi-You Huang,et al.  RCA combined nanoparticle-based optical detection technique for protein microarray: a novel approach. , 2004, Biosensors & bioelectronics.

[12]  A. Fire,et al.  Rolling replication of short DNA circles. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[13]  Michael J. Natan,et al.  SURFACE PLASMON RESONANCE OF AU COLLOID-MODIFIED AU FILMS : PARTICLE SIZE DEPENDENCE , 1999 .

[14]  P. Lizardi,et al.  Mutation detection and single-molecule counting using isothermal rolling-circle amplification , 1998, Nature Genetics.

[15]  Z. Kam,et al.  Absorption and Scattering of Light by Small Particles , 1998 .