Anticancer activity of lectins from Bauhinia purpurea and Wisteria floribunda on Breast cancer MCF-7 cell lines.

BACKGROUND Individual and collaborative efforts are being made worldwide in search of effective chemical or natural drugs with less severe side-effects for treatment of cancer. Due to the specificity and selectivity properties of lectins for saccharides, several plant lectins are known to induce cytotoxicity into tumor cells. OBJECTIVE To study the antiproliferative activity of two N-acetyl galactosamine specific plant lectins from seeds of Bauhinia purpurea and Wisteria floribunda against MCF-7 Breast cancer cell lines. METHODS MTT, lactate dehydrogenase (LDH) leakage, reactive oxygen species (ROS), and caspase-3 assays and flow cytometry for cell cycle analysis were performed. RESULTS The agglutinins BPL and WFL; 446 μgml-1 (2.2 μM) and 329 μgml-1 (2.8 μM), respectively caused remarkable concentration-dependent antiproliferative effect on MCF-7. The effect was seen to be a consequence of binding of the lectin to the cell surface and triggering S and G2 phase arrest. Apoptosis induced was found to be associated with LDH leakage, cell cycle arrest and ROS generation. The apoptotic signal was observed to be amplified by activation of caspase-3 resulting in cell death. CONCLUSION The study provides a base for detailed investigation and further use of lectins in cancer studies.

[1]  M. E. Okur,et al.  Investigation of Antioxidant and Cytotoxic Activities of Opuntia ficus-indica (L.) Mill. Fruit extract , 2019, Istanbul Journal of Pharmacy.

[2]  S. Gaikwad,et al.  Investigation of structural and saccharide binding transitions of Bauhinia purpurea and Wisteria floribunda lectins. , 2019, Archives of biochemistry and biophysics.

[3]  P. Bisen,et al.  Chickpea Lectin Inhibits Human Breast Cancer Cell Proliferation and Induces Apoptosis Through Cell Cycle Arrest. , 2018, Protein Peptide Letters.

[4]  Seema Kumari,et al.  Reactive Oxygen Species: A Key Constituent in Cancer Survival , 2018, Biomarker insights.

[5]  D. Pinto,et al.  Plant Secondary Metabolites as Anticancer Agents: Successes in Clinical Trials and Therapeutic Application , 2018, International journal of molecular sciences.

[6]  T. García-Gasca,et al.  Phaseolus acutifolius Lectin Fractions Exhibit Apoptotic Effects on Colon Cancer: Preclinical Studies Using Dimethilhydrazine or Azoxi-Methane as Cancer Induction Agents , 2017, Molecules.

[7]  T. García-Gasca,et al.  Plant Lectins as Medical Tools against Digestive System Cancers , 2017, International journal of molecular sciences.

[8]  M. Puri,et al.  Balsamin induces apoptosis in breast cancer cells via DNA fragmentation and cell cycle arrest , 2017, Molecular and Cellular Biochemistry.

[9]  Yan-Hong Wang,et al.  The efficacy of WGA modified daunorubicin anti-resistant liposomes in treatment of drug-resistant MCF-7 breast cancer , 2017, Journal of drug targeting.

[10]  B. Youan,et al.  Current status of lectin-based cancer diagnosis and therapy , 2017 .

[11]  J. Burke,et al.  Molecular Basis for Recognition of the Cancer Glycobiomarker, LacdiNAc (GalNAc[β1→4]GlcNAc), by Wisteria floribunda Agglutinin* , 2016, The Journal of Biological Chemistry.

[12]  C. G. Suresh,et al.  Two Chitotriose-Specific Lectins Show Anti-Angiogenesis, Induces Caspase-9-Mediated Apoptosis and Early Arrest of Pancreatic Tumor Cell Cycle , 2016, PloS one.

[13]  L. Pilcher,et al.  Anticancer Agents from Diverse Natural Sources , 2014, Natural product communications.

[14]  C. K. Das,et al.  In vitro and in vivo antitumor effects of Peanut agglutinin through induction of apoptotic and autophagic cell death. , 2014, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[15]  P. Satheeshkumar,et al.  Purified mulberry leaf lectin (MLL) induces apoptosis and cell cycle arrest in human breast cancer and colon cancer cells. , 2012, Chemico-biological interactions.

[16]  Q. Yan,et al.  Astragalus membranaceus lectin (AML) induces caspase‐dependent apoptosis in human leukemia cells , 2012, Cell proliferation.

[17]  A. Leathem,et al.  Lectin microarray profiling of metastatic breast cancers. , 2011, Glycobiology.

[18]  Masakazu Yamamoto,et al.  Wisteria floribunda agglutinin‐positive mucin 1 is a sensitive biliary marker for human cholangiocarcinoma , 2010, Hepatology.

[19]  John A Timbrell,et al.  In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. , 2006, Toxicology letters.

[20]  E. D. de Mejia,et al.  Lectins as Bioactive Plant Proteins: A Potential in Cancer Treatment , 2005, Critical reviews in food science and nutrition.

[21]  N. Sharon,et al.  History of lectins: from hemagglutinins to biological recognition molecules. , 2004, Glycobiology.

[22]  S. Choi,et al.  Korean mistletoe lectin-induced apoptosis in hepatocarcinoma cells is associated with inhibition of telomerasevia mitochondrial controlled pathway independent of p53 , 2002, Archives of pharmacal research.

[23]  G. Salvesen,et al.  Caspase activation: the induced-proximity model. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[24]  T. Decker,et al.  A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. , 1988, Journal of immunological methods.

[25]  J. Bao,et al.  Plant lectins: potential antineoplastic drugs from bench to clinic. , 2010, Cancer letters.

[26]  R. Kiss,et al.  In vitro influence of lectins and neoglycoconjugates on the growth of three human sarcoma cell lines , 1999, Journal of Cancer Research and Clinical Oncology.