Viability and Virulence of Experimentally Stressed Nonculturable Salmonella typhimurium

ABSTRACT Maintenance of pathogenicity of viable but nonculturableSalmonella typhimurium cells experimentally stressed with UV-C and seawater, was investigated relative to the viability level of the cellular population. Pathogenicity, tested in a mouse model, was lost concomitantly with culturability, whereas cell viability remained undamaged, as determined by respiratory activity and cytoplasmic membrane and genomic integrities.

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