New methods for field collection of human skin volatiles and perspectives for their application in the chemical ecology of human–pathogen–vector interactions

SUMMARY Odours emitted by human skin are of great interest to biologists in many fields, with practical applications in forensics, health diagnostic tools and the ecology of blood-sucking insect vectors of human disease. Convenient methods are required for sampling human skin volatiles under field conditions. We experimentally compared four modern methods for sampling skin odours: solvent extraction, headspace solid-phase micro-extraction (SPME), and two new techniques not previously used for the study of mammal volatiles, contact SPME and dynamic headspace with a chromatoprobe design. These methods were tested and compared both on European subjects under laboratory conditions and on young African subjects under field conditions. All four methods permitted effective trapping of skin odours, including the major known human skin volatile compounds. In both laboratory and field experiments, contact SPME, in which the time of collection was restricted to 3 min, provided results very similar to those obtained with classical headspace SPME, a method that requires 45 min of collection. Chromatoprobe sampling also proved to be very sensitive, rapid and convenient for the collection of human-produced volatiles in natural settings. Both contact SPME and chromatoprobe design may considerably facilitate the study of human skin volatiles under field conditions, opening new possibilities for examining the olfactory cues mediating the host-seeking behaviour of mosquito vectors implicated in the transmission of major diseases.

[1]  A. Cork,et al.  Identification of electrophysiologically‐active compounds for the malaria mosquito, Anopheles gambiae, in human sweat extracts , 1996, Medical and veterinary entomology.

[2]  Allison M. Curran,et al.  Evaluation of selected sorbent materials for the collection of volatile organic compounds related to human scent using non-contact sampling mode. , 2011, Forensic science international.

[3]  Zainulabeuddin Syed,et al.  Acute olfactory response of Culex mosquitoes to a human- and bird-derived attractant , 2009, Proceedings of the National Academy of Sciences.

[4]  M. Charpentier,et al.  Critical thinking in the chemical ecology of mammalian communication: roadmap for future studies , 2012 .

[5]  C. Wysocki,et al.  Analyses of volatile organic compounds from human skin , 2008, The British journal of dermatology.

[6]  J. V. van Loon,et al.  Sensitivities of antennal olfactory neurons of the malaria mosquito, Anopheles gambiae, to carboxylic acids. , 1999, Journal of insect physiology.

[7]  J. Schmid,et al.  A Broad Diversity of Volatile Carboxylic Acids, Released by a Bacterial Aminoacylase from Axilla Secretions, as Candidate Molecules for the Determination of Human‐Body Odor Type , 2006, Chemistry & biodiversity.

[8]  U. Bernier,et al.  Analysis of human skin emanations by gas chromatography/mass spectrometry. 1. Thermal desorption of attractants for the yellow fever mosquito (Aedes aegypti) from handled glass beads. , 1999, Analytical chemistry.

[9]  Lauryn E. DeGreeff,et al.  Collection and identification of human remains volatiles by non-contact, dynamic airflow sampling and SPME-GC/MS using various sorbent materials , 2011, Analytical and bioanalytical chemistry.

[10]  Jörg-Peter Schnitzler,et al.  Practical approaches to plant volatile analysis. , 2006, The Plant journal : for cell and molecular biology.

[11]  Laurent Dormont,et al.  Human Skin Volatiles: A Review , 2013, Journal of Chemical Ecology.

[12]  V. Camel,et al.  Analysis of human male armpit sweat after fenugreek ingestion: Characterisation of odour active compounds by gas chromatography coupled to mass spectrometry and olfactometry. , 2011, Food chemistry.

[13]  Allison M. Curran,et al.  Canine human scent identifications with post-blast debris collected from improvised explosive devices. , 2010, Forensic science international.

[14]  J. Pawliszyn,et al.  In vivo sampling with solid phase microextraction. , 2007, Journal of biochemical and biophysical methods.

[15]  Allison M. Curran,et al.  Comparison of the Volatile Organic Compounds Present in Human Odor Using Spme-GC/MS , 2005, Journal of Chemical Ecology.

[16]  K. Furton,et al.  Comparison of the Volatile Organic Compounds from Different Biological Specimens for Profiling Potential * , 2013, Journal of forensic sciences.

[17]  C. Costantini,et al.  Electroantennogram and behavioural responses of the malaria vector Anopheles gambiae to human‐specific sweat components , 2001, Medical and veterinary entomology.

[18]  S. Powers,et al.  Identification of Human-Derived Volatile Chemicals that Interfere with Attraction of Aedes aegypti Mosquitoes , 2008, Journal of Chemical Ecology.

[19]  B. Knols,et al.  Limburger cheese as an attractant for the malaria mosquito Anopheles gambiae s.s. , 1996, Parasitology today.

[20]  T. V. van Beek,et al.  Identification of Olfactory Stimulants for Anopheles gambiae from Human Sweat Samples , 2000, Journal of Chemical Ecology.

[21]  F. Tomita,et al.  Foot odor due to microbial metabolism and its control. , 2006, Canadian journal of microbiology.

[22]  R T Cardé,et al.  Activation, orientation and landing of female Culex quinquefasciatus in response to carbon dioxide and odour from human feet: 3‐D flight analysis in a wind tunnel , 2011, Medical and veterinary entomology.

[23]  J. Pickett,et al.  Headspace Analysis in Chemical Ecology: Effects of Different Sampling Methods on Ratios of Volatile Compounds Present in Headspace Samples , 1998, Journal of Chemical Ecology.

[24]  Allison M. Curran,et al.  The Evaluation of Human Hand Odor Volatiles on Various Textiles: A Comparison Between Contact and Noncontact Sampling Methods *,† , 2011, Journal of forensic sciences.

[25]  S Haze,et al.  2-Nonenal newly found in human body odor tends to increase with aging. , 2001, The Journal of investigative dermatology.

[26]  R. Adams,et al.  Identification of Essential Oil Components By Gas Chromatography/Mass Spectrometry , 2007 .

[27]  K. Furton,et al.  Informe de Caso HUman sCenT deTeCTIon : a reVIeW of ITs deVeLoPmenTs and forensIC aPPLICaTIons , 2008 .

[28]  W. Takken,et al.  Selection of biting sites on a human host by Anopheles gambiae s.s., An. arabiensis and An. quadriannulatus , 1998 .

[29]  Robert P. Adams,et al.  Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy , 2001 .

[30]  W. Takken,et al.  Behavioural and electrophysiological responses of the malaria mosquito Anopheles gambiae Giles sensu stricto (Diptera: Culicidae) to human skin emanations , 2004, Medical and veterinary entomology.

[31]  S. Dötterl,et al.  Qualitative and quantitative analyses of flower scent in Silene latifolia. , 2005, Phytochemistry.

[32]  T. Lefèvre,et al.  Infection and body odours: evolutionary and medical perspectives. , 2009, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[33]  M. Kusuhara,et al.  Odor Associated with Aging , 2010 .

[34]  M. Fukuda,et al.  Elucidation of chemical compounds responsible for foot malodour , 1990, The British journal of dermatology.

[35]  Elena Lucchi,et al.  HS‐SPME‐GC‐MS analysis of body odor to test the efficacy of foot deodorant formulations , 2009, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[36]  Andrew I. Spielman,et al.  Analysis of characteristic human female axillary odors: Qualitative comparison to males , 1996, Journal of Chemical Ecology.

[37]  B. Knols,et al.  Selection of biting sites on man by two malaria mosquito species , 1995, Experientia.

[38]  Gui-hua Ruan,et al.  The study of fingerprint characteristics of the emanations from human arm skin using the original sampling system by SPME-GC/MS. , 2005, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[39]  R. Ignell,et al.  Smelling your way to food: can bed bugs use our odour? , 2012, Journal of Experimental Biology.

[40]  G. Gries,et al.  Identification of the Airborne Aggregation Pheromone of the Common Bed Bug, Cimex lectularius , 2008, Journal of Chemical Ecology.

[41]  D. Barnard,et al.  Analysis of human skin emanations by gas chromatography/mass spectrometry. 2. Identification of volatile compounds that are candidate attractants for the yellow fever mosquito (Aedes aegypti). , 2000, Analytical chemistry.