Fast Cyclic Square-Wave Voltammetry To Enhance Neurotransmitter Selectivity and Sensitivity.

Although fast-scan cyclic voltammetry (FSCV) has been widely used for in vivo neurochemical detection, the sensitivity and selectivity of the technique can be further improved. In this study, we develop fast cyclic square-wave voltammetry (FCSWV) as a novel voltammetric technique that combines large-amplitude cyclic square-wave voltammetry (CSWV) with background subtraction. A large-amplitude, square-shaped potential was applied to induce cycling through multiple redox reactions within a square pulse to increase sensitivity and selectivity when combined with a two-dimensional voltammogram. As a result, FCSWV was significantly more sensitive than FSCV ( n = 5 electrodes, two-way ANOVA, p = 0.0002). In addition, FCSWV could differentiate dopamine from other catecholamines (e.g., epinephrine and norepinephrine) and serotonin better than conventional FSCV. With the confirmation that FCSWV did not influence local neuronal activity, despite the large amplitude of the square waveform, it could monitor electrically induced phasic changes in dopamine release in rat striatum before and after injecting nomifensine, a dopamine reuptake inhibitor.

[1]  L. Sombers,et al.  Electrochemical Selectivity Achieved Using a Double Voltammetric Waveform and Partial Least Squares Regression: Differentiating Endogenous Hydrogen Peroxide Fluctuations from Shifts in pH. , 2018, Analytical chemistry.

[2]  B. J. Venton,et al.  Sawhorse Waveform Voltammetry for Selective Detection of Adenosine, ATP, and Hydrogen Peroxide , 2014, Analytical chemistry.

[3]  T. Glass,et al.  Selective amine recognition: development of a chemosensor for dopamine and norepinephrine. , 2004, Organic letters.

[4]  R. Wightman,et al.  Characterization of local pH changes in brain using fast-scan cyclic voltammetry with carbon microelectrodes. , 2010, Analytical chemistry.

[5]  Parastoo Hashemi,et al.  Ultrafast detection and quantification of brain signaling molecules with carbon fiber microelectrodes. , 2012, Analytical chemistry.

[6]  P. Garris,et al.  Real‐Time Measurement of Electrically Evoked Extracellular Dopamine in the Striatum of Freely Moving Rats , 1997, Journal of neurochemistry.

[7]  B. Jill Venton,et al.  Electrochemical Properties of Different Carbon‐Fiber Microelectrodes Using Fast‐Scan Cyclic Voltammetry , 2008 .

[8]  B. Herman,et al.  Measurement of intracellular calcium. , 1999, Physiological reviews.

[9]  R. Wightman,et al.  Fast-scan cyclic voltammetry of 5-hydroxytryptamine. , 1995, Analytical chemistry.

[10]  C. Blaha,et al.  Tracking tonic dopamine levels in vivo using multiple cyclic square wave voltammetry. , 2018, Biosensors & bioelectronics.

[11]  R. Wightman,et al.  Real-time measurement of dopamine fluctuations after cocaine in the brain of behaving rats. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[12]  C. Blaha,et al.  Monitoring In Vivo Changes in Tonic Extracellular Dopamine Level by Charge-Balancing Multiple Waveform Fast-Scan Cyclic Voltammetry. , 2016, Analytical chemistry.

[13]  Dong Pyo Jang,et al.  Paired pulse voltammetry for differentiating complex analytes. , 2012, The Analyst.

[14]  R. Wightman,et al.  Fast-scan voltammetry of biogenic amines. , 1988, Analytical chemistry.

[15]  J. Helfrick,et al.  Cyclic square wave voltammetry of single and consecutive reversible electron transfer reactions. , 2009, Analytical chemistry.

[16]  R. Wightman,et al.  Monitoring rapid chemical communication in the brain. , 2008, Chemical reviews.

[17]  R. Wightman,et al.  Resolving neurotransmitters detected by fast-scan cyclic voltammetry. , 2004, Analytical chemistry.

[18]  Michael L Heien,et al.  Fast-scan controlled-adsorption voltammetry for the quantification of absolute concentrations and adsorption dynamics. , 2013, Langmuir : the ACS journal of surfaces and colloids.

[19]  P. Garris Advancing neurochemical monitoring , 2010, Nature Methods.

[20]  K. Yoshimi,et al.  Dual use of rectangular and triangular waveforms in voltammetry using a carbon fiber microelectrode to differentiate norepinephrine from dopamine , 2017 .

[21]  Garret D Stuber,et al.  Overoxidation of carbon-fiber microelectrodes enhances dopamine adsorption and increases sensitivity. , 2003, The Analyst.

[22]  R. Wightman,et al.  Multivariate concentration determination using principal component regression with residual analysis. , 2009, Trends in analytical chemistry : TRAC.

[23]  R. M. Wightman,et al.  Rapid and Selective Cyclic Voltammetric Measurements of Epinephrine and Norepinephrine as a Method To Measure Secretion from Single Bovine Adrenal Medullary Cells , 1994 .

[24]  R. McCreery,et al.  Control of Electron Transfer Kinetics at Glassy Carbon Electrodes by Specific Surface Modification , 1996 .

[25]  R. Wightman,et al.  Subsecond adsorption and desorption of dopamine at carbon-fiber microelectrodes. , 2000, Analytical chemistry.

[26]  Christopher J. Kimble,et al.  Wireless fast-scan cyclic voltammetry to monitor adenosine in patients with essential tremor during deep brain stimulation. , 2012, Mayo Clinic proceedings.

[27]  Adam K Dengler,et al.  Microfabricated Microelectrode Sensor for Measuring Background and Slowly Changing Dopamine Concentrations. , 2013, Journal of electroanalytical chemistry.